Abstract: The invention is related to a mechanical x-y-axis adjustable stage, which is designed for microscopic electro-physiological studies. The stage adopted a three-layer flat-slab structure. The up-most layer was a bearing layer on which other equipment could be mounted. The middle layer and bottom layer were guide layers, and rails for x-axis or y-axis guiding were installed. In order to move the top layer smoothly along two-dimension relative to the bottom layer, the three layers were jointed using grooved rails. By moving the top layer accordingly along the x-axis and y-axis, biological sample (animal/cells) as well as other instruments such as electrophysiological probes and manipulators could be moved smoothly and flexibly, and specific visual fields could be defined without moving the complex microscope system as well as other optical components. Therefore, the present invention has broad application values, for example two-electrodes electrophysiological recordings.