Abstract: A screening method of an autolysosome gene C10ORF10 for regulating fat function of obese patients is provided, including collecting subcutaneous and visceral adipose tissues from people with different metabolic conditions and different BMIs, followed by transcriptome sequencing analysis; dividing specimens from people with normal metabolism into two groups of BMI<25 kg/m2 and BMI>30 kg/m2, comparing the two groups in terms of expression differences of autolysosome-related genes in subcutaneous and visceral adipose tissues, screening out differential genes; analyzing and observing several autolysosome-related genes in terms of differential expressions existed in the subcutaneous and visceral adipose tissues of the two groups of BMI<25 kg/m2 and BMI>30 kg/m2, where a gene with a greatest expression difference is C10ORF10.