Abstract: A method of breeding a hypoxia-tolerant Megalobrama amblycephala strain, including: (1) screening genes associated with hypoxia tolerance in Megalobrama amblycephala based on transcriptome data; (2) screening SNP sites from the genes associated with hypoxia tolerance; and (3) applying a haplotype of the SNP sites in the molecular marker-assisted breeding of Megalobrama amblycephala to obtain the hypoxia-tolerant Megalobrama amblycephala strain. The genes associated with hypoxia tolerance include Epo, Hif1?, Hif2?, VhI, Hif1an, Vegfaa Egln1a, Egln1b, Egln2 and Egln3, and the haplotype of the SNP sites is T397T715 of gene Egln2.

Abstract: An expression method of active goldfish gfTP1 transposase protein comprises: constructing an expression vector comprising a gfTP1 transposase reading frame of a goldfish Tgf2 transposon, after transferred into escherichia coli Rosetta 1 (DE3), culturing an expression strain until absorbance of a bacteria solution under OD600 reaches 0.3-0.4, adding IPTG, culturing under 21-22° C. in shaking of 150-200 rpm, inducing to express soluble recombinant protein, and purifying to obtain a functionally active transposase. Also provided are an expression plasmid and the expression strain of the goldfish gfTP1 transposase protein, and a use of the strain in transgenosis.

Abstract: An expression method of active goldfish gfTP1 transposase protein comprises: constructing an expression vector comprising a gfTP1 transposase reading frame of a goldfish Tgf2 transposon, after transferred into escherichia coli Rosetta 1(DE3), culturing an expression strain until absorbance of a bacteria solution under OD600 reaches 0.3-0.4, adding IPTG, culturing under 21-22° C. in shaking of 150-200 rpm, inducing to express soluble recombinant protein, and purifying to obtain a functionally active transposase. Also provided are an expression plasmid and the expression strain of the goldfish gfTP1 transposase protein, and a use of the strain in transgenosis.