Patents by Inventor Shun-ichi Sekine

Shun-ichi Sekine has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Modified tRNA containing unnatural base and use thereof
    Patent number: 8715984
    Abstract: In the method for introducing a noncanonical amino acid residue into a desired position in a protein, the structure of tRNA is so modified as to have improved affinity for aminoacyl-tRNA synthetase or improved specificity to aminoacyl-tRNA synthetase. An unnatural base is contained at any position in tRNA, whereby the efficiency of aminoacylation of the tRNA with a noncanonical amino acid can be improved.
    Type: Grant
    Filed: February 3, 2009
    Date of Patent: May 6, 2014
    Assignee: Riken
    Inventors: Shun-ichi Sekine, Ryuya Fukunaga, Shigeyuki Yokoyama, Ichiro Hirao, Yoko Harada
  • Mutant SepRS, and method for site-specific introduction of phosphoserine into protein using the same
    Patent number: 8178301
    Abstract: A mutant SepRS which is suitable for a site-specific introduction of phosphoserine into a protein is prepared by analyzing the structure and functions of a phosphoseryl-tRNA synthetase (SepRS) derived from an archaebacterium. A mutant SepRS composed of an amino acid sequence depicted in SEQ ID NO:2, in which any one or more of glutamic acids at position-418 and position-420 and threonine at position-423 are substituted with other amino acid, and having enhanced binding affinity with a suppressor tRNA as compared with a wild type phosphoseryl-tRNA synthetase (SepRS) composed of an amino acid sequence depicted in SEQ ID NO:2 is provided.
    Type: Grant
    Filed: December 24, 2008
    Date of Patent: May 15, 2012
    Assignee: Riken
    Inventors: Shigeyuki Yokoyama, Ryuya Fukunaga, Shun-ichi Sekine
  • MODIFIED tRNA CONTAINING UNNATURAL BASE AND USE THEREOF
    Publication number: 20100323364
    Abstract: In the method for introducing a noncanonical amino acid residue into a desired position in a protein, the structure of tRNA is so modified as to have improved affinity for aminoacyl-tRNA synthetase or improved specificity to aminoacyl-tRNA synthetase. An unnatural base is contained at any position in tRNA, whereby the efficiency of aminoacylation of the tRNA with a noncanonical amino acid can be improved.
    Type: Application
    Filed: February 3, 2009
    Publication date: December 23, 2010
    Applicants: THE UNIVERSITY OF TOKYO, RIKEN
    Inventors: Shun-ichi Sekine, Ryuya Fukunaga, Shigeyuki Tokoyama, Ichiro Hirao, Yoko Harada
  • Mutant SepRS, and method for site-specific introduction of phosphoserine into protein using the same
    Publication number: 20090233290
    Abstract: A mutant SepRS which is suitable for a site-specific introduction of phosphoserine into a protein is prepared by analyzing the structure and functions of a phosphoseryl-tRNA synthetase (SepRS) derived from an archaebacterium. A mutant SepRS composed of an amino acid sequence depicted in SEQ ID NO:2, in which any one or more of glutamic acids at position-418 and position-420 and threonine at position-423 are substituted with other amino acid, and having enhanced binding affinity with a suppressor tRNA as compared with a wild type phosphoseryl-tRNA synthetase (SepRS) composed of an amino acid sequence depicted in SEQ ID NO:2 is provided.
    Type: Application
    Filed: December 24, 2008
    Publication date: September 17, 2009
    Applicants: RIKEN, THE UNIVERSITY OF TOKYO
    Inventors: Shigeyuki Yokoyama, Ryuya Fukunaga, Shun-ichi Sekine