Abstract: A tumor vaccine which comprises a microparticle or a lysate prepared from a solidified tumor material selected from the group consisting of a tumor tissue, a tumor cell, and a component thereof, and at least one cytokine and/or cytokine-inducing agent (e.g., a granulocyte-macrophage-colony stimulating factor and/or interleukin-2 and the like), and optionally an adjuvant. The vaccine can be easily prepared and widely applied for prevention of recurrence, inhibition of metastasis and therapeutic treatment regardless of a type of a tumor, and has excellent antitumor effect.

Abstract: A process for an induction culture of a cytotoxic T lymphocyte having cell killing activities against a tumor cell which comprises the step of coculturing a tumor tissue containing said tumor cell and a lymphocyte of autologous peripheral blood obtained from a subject from which the tumor tissue is derived. The process is convenient since no separation and pure culture of tumor cells is required. The resulting cytotoxic T lymphocyte cells are useful for clinical treatments of tumors, e.g. adoptive immunotherapies.