Patents by Inventor Shusaku Yanagidani
Shusaku Yanagidani has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 9994827Abstract: Object An object of the present invention is to provide a highly stable FVHO preparation and a low-hygroscopicity dried FVHO preparation. Means for achieving the object A method for producing a FVHO preparation comprising a step of allowing at least one member selected from phosphoric acid, casein peptone, D-glucosamine hydrochloride, melibiose, sorbose, lactose, fructose, melezitose, glucono-1,5-lactone, and ribitol; and a method for producing a dried FVHO preparation, comprising a step of allowing Bicine to coexist.Type: GrantFiled: January 10, 2013Date of Patent: June 12, 2018Assignee: TOYOBO CO., LTD.Inventors: Rie Hirao, Takahide Kishimoto, Shusaku Yanagidani
-
Patent number: 9796963Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity and that is suitable for use in SMBG. The present invention provides a flavin-bound glucose dehydrogenase having the following characteristics (1) to (5): (1) Temperature stability: stable at a temperature of 45° C. or lower; (2) stable at a pH range of 4.5 to 7.5; (3) substrate specificity: the reactivity to D-xylose, maltose, or D-galactose is 2% or less, based on the reactivity to D-glucose taken as 100%; (4) optimal activity temperature: 34 to 47° C.; and (5) optimal activity pH: 6.3 to 6.7.Type: GrantFiled: September 6, 2013Date of Patent: October 24, 2017Assignee: Toyobo Co., Ltd.Inventors: Yasuhiro Yamazaki, Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
-
Patent number: 9506043Abstract: A novel diaphorase; a method for producing the diaphorase; and use of the diaphorase are provided. The diaphorase comprises any one of the following polypeptides (a) to (c): (a) a polypeptide having the amino acid sequence of SEQ ID NO: 1, (b) a polypeptide having the amino acid sequence of SEQ ID NO: 1 in which one or several amino acid residues are substituted, deleted, inserted, added, and/or inverted, and having diaphorase activity, and (c) a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having diaphorase activity.Type: GrantFiled: December 19, 2012Date of Patent: November 29, 2016Assignee: TOYOBO CO., LTD.Inventors: Sachio Iwai, Takahide Kishimoto, Seiji Takeshima, Shusaku Yanagidani
-
Patent number: 9506042Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity, specific activity, thermal stability, and the like, and that is suitable for use in SMBG sensors. The present invention provides a purified polypeptide comprising an amino acid sequence having at least 80% identity to the sequence of SEQ ID NO: 1 and having glucose dehydrogenase activity.Type: GrantFiled: December 29, 2014Date of Patent: November 29, 2016Assignee: Toyobo Co., Ltd.Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroaki Kitazawa, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
-
Patent number: 9487758Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: GrantFiled: February 10, 2014Date of Patent: November 8, 2016Assignee: Toyobo Co., Ltd.Inventors: Yosuke Sumida, Hiroshi Aiba, Hiroshi Kawaminami, Rie Hirao, Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani
-
Patent number: 9404144Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55° C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.Type: GrantFiled: February 7, 2013Date of Patent: August 2, 2016Assignee: TOYOBO CO., LTD.Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Kawaminami, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
-
Patent number: 9260699Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: GrantFiled: April 30, 2014Date of Patent: February 16, 2016Assignee: Toyobo Co., Ltd.Inventors: Yosuke Sumida, Hiroshi Aiba, Hiroshi Kawaminami, Rie Hirao, Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani
-
Publication number: 20150344852Abstract: [Object] To provide: a novel diaphorase; a method for producing the diaphorase; and use of the diaphorase. [Solution] A diaphorase comprising any one of the following polypeptides (a) to (c): (a) a polypeptide having the amino acid sequence of SEQ ID NO: 1, (b) a polypeptide having the amino acid sequence of SEQ ID NO: 1 in which one or several amino acid residues are substituted, deleted, inserted, added, and/or inverted, and having diaphorase activity, and (c) a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having diaphorase activity.Type: ApplicationFiled: December 19, 2012Publication date: December 3, 2015Applicants: TOYOBO CO., LTD., TOYOBO CO., LTD.Inventors: Sachio IWAI, Takahide KISHIMOTO, Seiji TAKESHIMA, Shusaku YANAGIDANI
-
Publication number: 20150240216Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity and that is suitable for use in SMBG. The present invention provides a flavin-bound glucose dehydrogenase having the following characteristics (1) to (5): (1) Temperature stability: stable at a temperature of 45° C. or lower; (2) stable at a pH range of 4.5 to 7.5; (3) substrate specificity: the reactivity to D-xylose, maltose, or D-galactose is 2% or less, based on the reactivity to D-glucose taken as 100%; (4) optimal activity temperature: 34 to 47° C.; and (5) optimal activity pH: 6.3 to 6.7.Type: ApplicationFiled: September 6, 2013Publication date: August 27, 2015Inventors: Yasuhiro Yamazaki, Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
-
Patent number: 9115051Abstract: A method for producing a polyamine composition with high production efficiency that has a low salt concentration includes (1) a step of treating a plant and/or a processed plant product with ethanol; (2) a step of treating the plant and/or the processed plant product with water; (3) a step of treating the plant and/or the processed plant product under an acidic condition; and (4) a step of separating and collecting a liquid fraction.Type: GrantFiled: September 28, 2012Date of Patent: August 25, 2015Assignee: TOYOBO CO., LTD.Inventors: Chiaki Yamada, Hiroaki Kitazawa, Shusaku Yanagidani
-
Patent number: 9040257Abstract: The present invention provides a host for genetic recombination useful in the production of heterologous proteins in a large scale, by suppressing the production of extracellular polysaccharides in the basidiomycetous yeasts. Cryptococcus sp. S-2 D11 strain (FERM BP-11482) which is characterized in that the production of extracellular polysaccharides is suppressed as compared with the parent strain.Type: GrantFiled: April 23, 2013Date of Patent: May 26, 2015Assignees: TOYOBO CO., LTD., NATIONAL RESEARCH INSTITUTE OF BREWINGInventors: Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani, Kazuo Masaki
-
Publication number: 20150111280Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity, specific activity, thermal stability, and the like, and that is suitable for use in SMBG sensors. The present invention provides a purified polypeptide comprising an amino acid sequence having at least 80% identity to the sequence of SEQ ID NO: 1 and having glucose dehydrogenase activity.Type: ApplicationFiled: December 29, 2014Publication date: April 23, 2015Inventors: Yosuke SUMIDA, Rie HIRAO, Yuu UTASHIMA, Hiroaki KITAZAWA, Hiroshi AIBA, Takahide KISHIMOTO, Shusaku YANAGIDANI
-
Publication number: 20150072379Abstract: The present invention provides a host for genetic recombination useful in the production of heterologous proteins in a large scale, by suppressing the production of extracellular polysaccharides in the basidiomycetous yeasts. Cryptococcus sp. S-2 D11 strain (FERN BP-11482) which is characterized in that the production of extracellular polysaccharides is suppressed as compared with the parent strain.Type: ApplicationFiled: April 23, 2013Publication date: March 12, 2015Applicants: TOYOBO CO., LTD., NATIONAL RESEARCH INSTITUTE OF BREWINGInventors: Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani, Kazuo Masaki
-
Publication number: 20150031059Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55° C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.Type: ApplicationFiled: February 7, 2013Publication date: January 29, 2015Applicant: TOYOBO CO., LTD.Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Kawaminami, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
-
Publication number: 20140378708Abstract: [Object] An object is to provide a method for producing a polyamine composition with high production efficiency that has a low salt concentration and is derived from plant material. [Solution] A method for producing a polyamine composition includes (1) a step of treating a plant and/or a processed plant product with ethanol; (2) a step of treating the plant and/or the processed plant product with water; (3) a step of treating the plant and/or the processed plant product under an acidic condition; and (4) a step of separating and collecting a liquid fraction.Type: ApplicationFiled: September 28, 2012Publication date: December 25, 2014Applicant: TOYOBO CO., LTD.Inventors: Chiaki Yamada, Hiroaki Kitazawa, Shusaku Yanagidani
-
Publication number: 20140349327Abstract: Object An object of the present invention is to provide a highly stable FVHO preparation and a low-hygroscopicity dried FVHO preparation. Means for achieving the object A method for producing a FVHO preparation comprising a step of allowing at least one member selected from phosphoric acid, casein peptone, D-glucosamine hydrochloride, melibiose, sorbose, lactose, fructose, melezitose, glucono-1,5-lactone, and ribitol; and a method for producing a dried FVHO preparation, comprising a step of allowing Bicine to coexist.Type: ApplicationFiled: January 10, 2013Publication date: November 27, 2014Applicant: TOYOBO CO., LTD.Inventors: Rie Hirao, Takahide Kishimoto, Shusaku Yanagidani
-
Publication number: 20140287478Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: ApplicationFiled: April 30, 2014Publication date: September 25, 2014Inventors: Yosuke SUMIDA, Hiroshi AIBA, Hiroshi KAWAMINAMI, Rie HIRAO, Yuu UTASHIMA, Takahide KISHIMOTO, Shusaku YANAGIDANI
-
Publication number: 20140154777Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: ApplicationFiled: February 10, 2014Publication date: June 5, 2014Applicant: TOYOBO CO., LTD.Inventors: Yosuke SUMIDA, Hiroshi AIBA, Hiroshi KAWAMINAMI, Rie HIRAO, Yuu UTASHIMA, Takahide KISHIMOTO, Shusaku YANAGIDANI
-
Patent number: 7754864Abstract: Disclosed is a tyrosinase activity controlling agent comprising, as an active ingredient, a compound that has tyrosinase inhibiting or promoting activity, an external preparation comprising the controlling agent, and a process for producing the compound.Type: GrantFiled: October 10, 2003Date of Patent: July 13, 2010Assignees: National Institute of Advanced Industrial Science and Technology, Toyo Boseki Kabushiki KaishaInventors: Yutaka Tokiwa, Masaru Kitagawa, Kenji Yoshino, Shusaku Yanagidani, Takao Raku, Tetsuzo Totani, Hiromi Shimakawa
-
Patent number: 7264955Abstract: An isolated, porcine ?1-6 fucosyltransferase having the following physico-chemical properties: (1) action: transferring fucose from guanosine diphosphate-fucose to a hydroxy group at 6-position of GluNAc closest to R of a receptor (GlcNAc?1-2Man?1-6)(GlcNAc?1-2Man?1-3)Man?1-4GlcNAc?1-4GlucNAc-R wherein R is an asparagine residue or a peptide chain carrying said residue, whereby to form (GlcNAc?1-2Man?1-6)-(GlcNAc?1-2Man?1-3)Man?1-4GlcNAc?1-4(Fuc?1-6)GlucNAc-R (2) optimum pH: about 7.0 (3) pH stability: retains activity after 5 hours of treatment at 4° C. at a pH range of 4.0-10.0 (4) optimum temperature: about 30-37° C. (5) inhibition or activation: no requirement for divalent metal for expression of activity; no inhibition of activity in the presence of 5 mM EDTA (6) molecular weight: about 60,000 by SDS-polyacrylamide gel electrophoresis is provided. An isolated polynucleotide encoding porcine ?1-6 fucosyltransferase is also provided.Type: GrantFiled: May 13, 2004Date of Patent: September 4, 2007Assignee: Toyo Boseki Kabushiki KaishaInventors: Naoyuki Taniguchi, Naofumi Uozumi, Tetsuo Shiba, Shusaku Yanagidani