Patents by Inventor Shusei Obata

Shusei Obata has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10894967
    Abstract: The present disclosure provides a novel aldehyde synthase gene having activity of synthesizing a medium-chain aldehyde. Such gene encodes a protein comprising the amino acid sequence as shown in SEQ ID NO: 2.
    Type: Grant
    Filed: March 3, 2020
    Date of Patent: January 19, 2021
    Assignees: Toyota Jidosha Kabushiki Kaisha, Kyoto University
    Inventors: Masayoshi Muramatsu, Shusei Obata, Masakazu Ito, Jun Ogawa, Shigenobu Kishino
  • Patent number: 10894955
    Abstract: The present disclosure is intended to identify a substitution mutation that improves enzyme activity of a decarbonylase. An amino acid corresponding to an amino acid 90, 107, 163, or 171 in SEQ ID NO: 2 is to be substituted with an amino acid exhibiting a higher degree of hydrophobicity.
    Type: Grant
    Filed: January 30, 2020
    Date of Patent: January 19, 2021
    Assignee: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Masayoshi Muramatsu, Shusei Obata
  • Publication number: 20200283804
    Abstract: The present disclosure provides a novel aldehyde synthase gene having activity of synthesizing a medium-chain aldehyde. Such gene encodes a protein comprising the amino acid sequence as shown in SEQ ID NO: 2.
    Type: Application
    Filed: March 3, 2020
    Publication date: September 10, 2020
    Applicants: Toyota Jidosha Kabushiki Kaisha, Kyoto University
    Inventors: Masayoshi Muramatsu, Shusei Obata, Masakazu Ito, Jun Ogawa, Shigenobu Kishino
  • Publication number: 20200248164
    Abstract: The present disclosure is intended to identify a substitution mutation that improves enzyme activity of a decarbonylase. An amino acid corresponding to an amino acid 90, 107, 163, or 171 in SEQ ID NO: 2 is to be substituted with an amino acid exhibiting a higher degree of hydrophobicity.
    Type: Application
    Filed: January 30, 2020
    Publication date: August 6, 2020
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Masayoshi MURAMATSU, Shusei OBATA
  • Publication number: 20200165619
    Abstract: The present disclosure is intended to identify a substitution mutation that improves enzyme activity of a decarbonylase. Such substitution mutation is implemented at valine at position 29, glutamic acid at position 35, asparagine at position 39, threonine at position 42, histidine at position 51, leucine at position 54, methionine at position 60, serine at position 89, asparagine at position 94, leucine at position 169, asparagine at position 174, leucine at position 175, isoleucine at position 177, or aspartic acid at position 188 in the amino acid sequence as shown in SEQ ID NO: 2.
    Type: Application
    Filed: November 20, 2019
    Publication date: May 28, 2020
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Masayoshi Muramatsu, Shusei Obata
  • Publication number: 20180201910
    Abstract: The capacity for alkane synthesis is to be significantly improved with the use of an acyl-ACP reductase gene and a decarbonylase gene derived from blue-green algae. A recombinant microorganism comprising, as foreign genes, an acyl-ACP reductase gene and a decarbonylase gene derived from blue-green algae comprises 4 types of ferredoxin genes and 3 types of ferredoxin reductase genes derived from blue-green algae in predetermined combinations.
    Type: Application
    Filed: January 11, 2018
    Publication date: July 19, 2018
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Masayoshi MURAMATSU, Shusei OBATA
  • Patent number: 9005953
    Abstract: This invention relates to a recombinant microorganism having improved butanol production capacity and butanol production with the use of such recombinant microorganism with good efficiency. In this invention, the acetoacetyl-CoA synthase gene encoding an enzyme capable of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA and a group of genes involved in butanol biosynthesis that enables synthesis of butanol from acetoacetyl-CoA are introduced into a host microorganism.
    Type: Grant
    Filed: May 6, 2010
    Date of Patent: April 14, 2015
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Masayoshi Muramatsu, Shusei Obata, Satoshi Yoneda, Masahiro Sugimura, Tomohisa Kuzuyama
  • Patent number: 8759046
    Abstract: A method of producing a prenyl alcohol(s) by culturing a mutant cell into which a fusion gene of farnesyl diphosphate synthase gene and geranylgeranyl diphosphate synthase gene has been introduced and recovering the prenyl alcohol(s) from the resultant culture.
    Type: Grant
    Filed: April 1, 2008
    Date of Patent: June 24, 2014
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Chikara Ohto, Shusei Obata
  • Patent number: 8748137
    Abstract: It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material.
    Type: Grant
    Filed: October 27, 2009
    Date of Patent: June 10, 2014
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Shusei Obata, Hiromi Kambe, Masakazu Ito, Takashi Shimamura, Katsunori Kohda, Seiichi Taguchi
  • Patent number: 8563281
    Abstract: The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate via microbial fermentation with the use of a sugar as a starting material. The method may comprise culturing a recombinant microorganism expressing: a protein capable of catalyzing the transfer of CoA to propionic acid and/or lactate; a protein capable of catalyzing the formation of acetoacetyl-CoA from two acetyl-CoA molecules; a protein capable of catalyzing acetoacetyl-CoA reduction; and a protein capable of catalyzing polyhydroxyalkanoate synthesis. According to the production method, a polyester copolymer consisting of 3-hydroxybutyrate and lactate can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.
    Type: Grant
    Filed: April 23, 2009
    Date of Patent: October 22, 2013
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Seiichi Taguchi, Kenji Tajima, Yasuharu Satoh, Ken'ichiro Matsumoto, Miwa Yamada, Shusei Obata, Hiromi Kambe, Katsunori Kohda, Katsuhiro Ohno, Takashi Shimamura
  • Patent number: 8541213
    Abstract: This invention provides a recombinant yeast that can produce branched alcohol appropriate for automotive fuel and the like and a branched alcohol production method whereby branched alcohol can be produced at low cost with the use of the recombinant yeast. A recombinant yeast in which a hydroxymethyl glutaryl-CoA reductase gene has been expressed to a high degree and the ADP-ribose pyrophosphatase gene and/or the yhfR gene are introduced so as to be expressed therein is provided.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: September 24, 2013
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Satoshi Yoneda, Masayoshi Muramatsu, Shusei Obata, Ai Inoue
  • Publication number: 20130011892
    Abstract: This invention provides a recombinant yeast that can produce branched alcohol appropriate for automotive fuel and the like and a branched alcohol production method whereby branched alcohol can be produced at low cost with the use of the recombinant yeast. A recombinant yeast in which a hydroxymethyl glutaryl-CoA reductase gene has been expressed to a high degree and the ADP-ribose pyrophosphatase gene and/or the yhfR gene are introduced so as to be expressed therein is provided.
    Type: Application
    Filed: September 14, 2012
    Publication date: January 10, 2013
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Satoshi YONEDA, Masayoshi MURAMATSU, Shusei OBATA, Ai INOUE
  • Publication number: 20120122165
    Abstract: This invention provides a recombinant microorganism exhibiting excellent aliphatic polyester productivity and a method for producing an aliphatic polyester with the use of the recombinant microorganism. In this invention, the Megasphaera elsdenii-derived pct gene (and/or the Staphylococcus aureus-derived pct gene) and the Pseudomonas sp. 61-3 strain-derived PHA synthase gene (phaC2 gene) (and/or the Alcanivorax borkumensis SK2 strain-derived PHA synthase gene) are introduced into a host microorganism.
    Type: Application
    Filed: July 27, 2010
    Publication date: May 17, 2012
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Shusei Obata, Masayoshi Muramatsu, Hiromi Kambe, Masakazu Ito, Takashi Shimamura, Katsunori Kohda
  • Publication number: 20120100062
    Abstract: A combined plant is provided. The combined plant of continuously supplying hydrogen and nitrogen to an ammonia synthesis facility that continuously synthesizes ammonia from hydrogen and nitrogen, the combined plant including: a hydrogen production facility for acquiring solar energy and producing hydrogen by utilizing a part of the acquired solar energy; a nitrogen production facility for producing nitrogen from air and supplying the nitrogen to the ammonia synthesis facility; and a hydrogen storage facility for storing the hydrogen produced by the hydrogen production facility and supplying the produced hydrogen to the ammonia synthesis facility.
    Type: Application
    Filed: April 28, 2010
    Publication date: April 26, 2012
    Inventors: Norihiko Nakamura, Shigeki Sugiura, Shusei Obata, Shinichi Takeshima, Haruyuki Nakanishi, Yosuke Iida, Akinori Sato
  • Publication number: 20110212497
    Abstract: It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material.
    Type: Application
    Filed: October 27, 2009
    Publication date: September 1, 2011
    Applicants: National University Corporation Hokkaido University, Toyota Jidosha Kabushiki Kaisha
    Inventors: Shusei Obata, Hiromi Kambe, Masakazu Ito, Takashi Shimamura, Katsunori Kohda, Seiichi Taguchi
  • Publication number: 20110104768
    Abstract: The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3HB and LA via microbial fermentation with the use of a sugar as a starting material.
    Type: Application
    Filed: April 23, 2009
    Publication date: May 5, 2011
    Applicants: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY, TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Seiichi Taguchi, Kenji Tajima, Yasuharu Satoh, Ken'ichiro Matsumoto, Miwa Yamada, Shusei Obata, Hiromi Kambe, Katsuhiro Kohda, Katsuhiro Ohno, Takashi Shimamura
  • Publication number: 20100324282
    Abstract: This invention provides a method for separating a heteropolyacid from a monosaccharide in the presence of water. The method comprises separating a heteropolyacid from a mixture containing a monosaccharide, the heteropolyacid and water using an organic solvent selected from the group consisting of linear C2-4 alkyl ethyl ether and linear or branched C6-12 alcohol.
    Type: Application
    Filed: June 16, 2010
    Publication date: December 23, 2010
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Masayoshi MURAMATSU, Satoshi Yoneda, Tsuruyo Shimazaki, Shusei Obata
  • Publication number: 20100311136
    Abstract: This invention provides a recombinant yeast that can produce branched alcohol appropriate for automotive fuel and the like and a branched alcohol production method whereby branched alcohol can be produced at low cost with the use of the recombinant yeast. A recombinant yeast in which a hydroxymethyl glutaryl-CoA reductase gene has been expressed to a high degree and the ADP-ribose pyrophosphatase gene and/or the yhfR gene are introduced so as to be expressed therein is provided.
    Type: Application
    Filed: June 4, 2010
    Publication date: December 9, 2010
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: SATOSHI YONEDA, MASAYOSHI MURAMATSU, SHUSEI OBATA, AI INOUE
  • Publication number: 20100285549
    Abstract: This invention relates to a recombinant microorganism having improved butanol production capacity and butanol production with the use of such recombinant microorganism with good efficiency. In this invention, the acetoacetyl-CoA synthase gene encoding an enzyme capable of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA and a group of genes involved in butanol biosynthesis that enables synthesis of butanol from acetoacetyl-CoA are introduced into a host microorganism.
    Type: Application
    Filed: May 6, 2010
    Publication date: November 11, 2010
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: MASAYOSHI MURAMATSU, SHUSEI OBATA, SATOSHI YONEDA, MASAHIRO SUGIMURA, TOMOHISA KUZUYAMA
  • Patent number: 7501268
    Abstract: A method of producing a prenyl alcohol, comprising creating a recombinant by transferring into a host a recombinant DNA for expression or a DNA for genomic integration each comprising a prenyl diphosphate synthase gene or a mutant thereof, culturing the resultant recombinant, and recovering the prenyl alcohol from the resultant culture.
    Type: Grant
    Filed: December 20, 2001
    Date of Patent: March 10, 2009
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Chikara Ohto, Shusei Obata, Masayoshi Muramatsu, Kiyohiko Nishi, Kazuhiko Totsuka