Abstract: A method and apparatus for entering the structures of assumed sugar chains, assumed ionic numbers, m/z of a common product ion, etc. for calculating, by a precursor m/z calculator the m/z of precursor ions originating from each sugar chain. A method file creator prepares a method file including MRM transitions. A multivalent ion information file creator creates a file which associates a unique ID of each sugar-chain structure with m/z of precursor ions and m/z of a product ion. Then, a chromatogram creator creates a mass chromatogram for each MRM transition. A peak area totalizer adds up peak areas on mass chromatograms obtained for a plurality of MRM transitions corresponding to the same sugar chain. Based on the calculated total values, a quantitative value calculator calculates an abundance ratio of each sugar chain as a quantitative value. A quantitative output information creator displays the quantitative values on a display unit.

Abstract: A method and apparatus for entering the structures of assumed sugar chains, assumed ionic numbers, m/z of a common product ion, etc. for calculating, by a precursor m/z calculator the m/z of precursor ions originating from each sugar chain. A method file creator prepares a method file including MRM transitions. A multivalent ion information file creator creates a file which associates a unique ID of each sugar-chain structure with m/z of precursor ions and m/z of a product ion. Then, a chromatogram creator creates a mass chromatogram for each MRM transition. A peak area totalizer adds up peak areas on mass chromatograms obtained for a plurality of MRM transitions corresponding to the same sugar chain. Based on the calculated total values, a quantitative value calculator calculates an abundance ratio of each sugar chain as a quantitative value. A quantitative output information creator displays the quantitative values on a display unit.

Abstract: An object of the present invention is to provide a method for conveniently analyzing sugar chain (isomer) structure using a sample of approximately 1 picomole, which is generally subjected to analysis in proteomics without using any sugar chain preparation. The present invention relates to a method for analyzing sugar chain structure, comprising a step of obtaining the fragmentation pattern of a test sugar chain through fragmentation of the test sugar chain and a step of predicting the structure of the test sugar chain through comparison of the sugar chain predicted fragmentation pattern data generated based on fragmentation pattern templates with the fragmentation pattern of the test sugar chain.

Abstract: An object of the present invention is to provide a system for analyzing a sugar chain structure which can determine a complete primary structure of a sugar chain simply and rapidly with a small amount of sample.