Abstract: A microchannel electrophoresis-assisted micro-ultrasonic machining apparatus based on a 3D printing mold includes a working platform, a power supply, a 3D printing mold, a working solution tank and an ultrasonic vibration system. The working platform is configured to secure the apparatus. The working solution tank is provided with electrophoresis-assisted electrodes. The ultrasonic vibration system is secured to the lower end of the transfer module. The positive electrode of the electrophoretic DC power supply is electrically connected to the tool and the negative electrode is electrically connected to the electrophoresis-assisted electrodes. A workpiece to be processed is assembled with a 3D printing mold. After assembly, the workpiece and the 3D printing mold are placed in the middle of the electrophoresis-assisted electrodes inside the working solution tank and arranged to correspond to the tool.