Abstract: An electrically-driven pumping system and a driving method thereof. The electrically-driven pumping system includes: a power generation device, at least one energy storage system, a main motor, a pumping device, an auxiliary device, and a first transformer. The first transformer includes an input end, a first output end, and a second output end, the power generation device is connected to the energy storage unit, the input end of the first transformer is connected to the energy storage unit, the first output end of the first transformer is electrically connected to the main motor, and the second output end of the first transformer is electrically connected to the auxiliary device. As such, the electrically-driven pumping system may avoid problems, such as the shutdown of the main motor caused by power failure of the power generation device, and ensure a continuous operation.

Abstract: The present disclosure provides a fracturing device driven by a variable-frequency adjustable-speed integrated machine (VFASIM), including the VFASIM and a plunger pump. The VFASIM includes a driving device for providing a driving force and an inverting device integrally installed on the driving device. The inverting device supplies power to the driving device. The plunger pump is integrally installed together with the VFASIM, the plunger pump is mechanically connected to the driving device of the VFASIM and driven by the driving device. According to the present disclosure, it is possible to achieve an overall layout with a high degree of integration. The present disclosure also provides a well site layout including a plurality of fracturing devices described above.

Abstract: A method for arterial endothelial-enhanced functional T cell generation is provided. In the method, arterial endothelial cells enhance functional T cell generation by promoting the generation of hematopoietic progenitor cells with T-lineage bias. The first stage of T cell differentiation from human pluripotent stem cells (hPSCs) is optimized, and it is found that hPSC-derived autologous arterial endothelial cells increase the T cell potential of hematopoietic progenitor cells. Moreover, the T cells generated by arterial endothelial cell priming share similar function to that of human peripheral blood T cells. hPSC-derived CD19-CAR-T cells have been verified to have tumor-killing effects both in vivo and in vitro. The established hPSC-T differentiation system would provide a valuable resource for chimeric antigen receptor T cell (CAR-T) therapy.