Abstract: The present invention relates to a DNA polymerase variant that belongs to family A, and to a use thereof. The present invention relates to: a DNA polymerase variant which easily undergoes polymerization when the base at the 3?-end of a primer is complementary to a template, and yet has inhibited polymerization when the base at the 3?-end of the primer is non-complementary to the template, and thus facilitates discrimination between the two cases; a PCR process using the variant; and a PCR kit comprising the variant. The present invention is useful for single nucleotide polymorphism analysis (SNP genotyping), somatic mutation detection, and the like.

Abstract: The present invention relates to a method and a kit for detecting alleles of which the specificity and sensitivity in a DNA polymerase chain reaction (PCR), which is widely used for the detection of minor alleles such as single nucleotide polymorphisms or somatic mutations, are increased. More specifically, the present invention relates to a PCR-based method and kit for single nucleotide polymorphism (SNP) genotyping and somatic mutation detection, the method and kit adding a partially or fully double-stranded oligonucleotide for increasing discrimination to a PCR solution for selective amplification of alleles, so that PCR amplification is not affected when a primer 3? terminal base is complementary (3?-matched) to a template but PCR amplification is strongly inhibited when a 3? terminal base is not complementary (3?-mismatched).

Abstract: Disclosed is a method for detecting single nucleotide polymorphism (SNP) using a feature that the 5?-flap endonuclease (FEN) activity of DNA polymerase is inhibited when a probe complementarily binds to the end of a polymerase chain reaction (PCR) product. More specifically, the present invention relates to a novel method wherein it was verified that, when a probe used for a real-time PCR complementarily binds to the end site of a PCR product, the 5?-FEN activity of thermostable DNA polymerase to the probe is inhibited, and thus when such a feature is used to make a design such that an SNP site to be detected is located at the 5?-end site of the probe, the 5?-flap formation is induced according to the allele, thereby allowing effective SNP detection.

Abstract: The present invention relates to a method for detecting single nucleotide polymorphism (SNP) using a feature that the 5?-flap endonuclease (FEN) activity of DNA polymerase is inhibited when a probe complementarily binds to the end of a polymerase chain reaction (PCR) product. More specifically, the present invention relates to a novel method wherein it was verified that, when a probe used for a real-time PCR complementarily binds to the end site of a PCR product, the 5?-FEN activity of thermostable DNA polymerase to the probe is inhibited, and thus when such a feature is used to make a design such that an SNP site to be detected is located at the 5?-end site of the probe, the 5?-flap formation is induced according to the allele, thereby allowing effective SNP detection.

Abstract: The present invention relates to novel tacrolimus analogs, a composition for the prevention or treatment of neurological diseases or immune hypersensitivity disorders comprising the same, a method for preventing or treating neurological diseases or immune hypersensitivity disorders comprising administering the analogs to a subject, a method for preparing the analogs using an isolated modified Streptomyces sp. strain wherein the activity of one or more enzymes selected from the group consisting of TcsA, TcsB, TcsC and TcsD is reduced; and the isolated modified Streptomyces sp. strain for prepare the analogs.

Abstract: The present invention relates to a method for detecting single nucleotide polymorphism (SNP) using a feature that the 5?-flap endonuclease (FEN) activity of DNA polymerase is inhibited when a probe complementarily binds to the end of a polymerase chain reaction (PCR) product. More specifically, the present invention relates to a novel method wherein it was verified that, when a probe used for a real-time PCR complementarily binds to the end site of a PCR product, the 5?-FEN activity of thermostable DNA polymerase to the probe is inhibited, and thus when such a feature is used to make a design such that an SNP site to be detected is located at the 5?-end site of the probe, the 5?-flap formation is induced according to the allele, thereby allowing effective SNP detection.

Abstract: The present invention relates to novel tacrolimus analogues, a composition for the prevention or treatment of neurological diseases or immune hypersensitivity disorders comprising the same, a method for preventing or treating neurological diseases or immune hypersensitivity disorders comprising administering the analogues to a subject, a method for preparing the analogues using an isolated modified Streptomyces sp. strain wherein the activity of one or more enzymes selected from the group consisting of TcsA, TcsB, TcsC and TcsD is reduced; and the isolated modified Streptomyces sp. strain for prepare the analogues.

Abstract: The present invention relates to a method for separating and purifying a lactone compound with an unsaturated alkyl group from a lactone compound with a saturated alkyl group which is an analog thereof. More specifically, the present invention relates to a method for effectively separating a lactone compound with an unsaturated alkyl group such as FK506 at high purity by extraction with a silver ion (Ag+) solution without use of column chromatography.

Abstract: The present invention relates to a method of fermenting and purifying tricyclo compounds, specifically FK506 and/or FK520, and more particularly, to a method of purifying tricyclo compounds by adding a hydrophobic absorbent resin as a carrier in culturing FK506 and/or FK520 producing bacteria.

Abstract: The present invention relates to a method for separating and purifying a lactone compound with an unsaturated alkyl group from a lactone compound with a saturated alkyl group which is an analog thereof. More specifically, the present invention relates to a method for effectively separating a lactone compound with an unsaturated alkyl group such as FK506 at high purity by extraction with a silver ion (Ag+) solution without use of column chromatography.