Abstract: Disclosed are an ampoule-fixing holder for an intraosseous anesthetic solution injection device and an anesthetic solution injection device including the same. The ampoule-fixing holder includes a cylindrical holder body including an open portion formed at the rear end thereof to allow an ampoule containing an anesthetic solution to be inserted thereinto or removed therefrom, a head configured to enable mounting of an injection needle to the front end of the holder body, a rotary body disposed between the head and the holder body to withstand rotational force and an axial load, and a fixing member configured to firmly hold the ampoule inserted into the holder body to effectively transmit rotational force to the ampoule.