Abstract: The present disclosure relates generally to a tubing apparatus for separating and concentrating stem and stromal cells, also known as regenerative cells, from adipose tissue, more specifically to a defined process of extracting, separating and concentrating clinically useful regenerative cells from adipose tissue using a combination of mechanical disruption and filtration-centrifugation to obtain a highly enriched heterogeneous population of stem stromal cells. The centrifuge tube comprising a threaded top cap with male luer access port to be adapted to female luer of a syringe, a tapered main tubular barrel, a thin disk filter, and a bottom conical cap with luer access port for withdrawal of stem stromal cells via a syringe.

Abstract: The present disclosure relates generally to processes, devices and systems for separating and concentrating stem and stromal cells from adipose tissue using a combination of mechanical disruption and filtration-centrifugation to obtain a highly enriched population of stem cells.

Abstract: Disclosed is a method of regulating the expression in a human or animal subject of a nucleic acid sequence encoding a polypeptide which is immunogenic in the subject; the method comprising introducing into a mammal a cell comprising the nucleic acid sequence encoding the immunogenic polypeptide, said sequence being operably linked to a drug-regulatable promoter; and altering the concentration of regulatory drug to which the cell is exposed.

Abstract: Disclosed is a method of regulating the expression in a human or animal subject of a nucleic acid sequence encoding a polypeptide which is immunogenic in the subject; the method comprising introducing into a mammal a cell comprising the nucleic acid sequence encoding the immunogenic polypeptide, said sequence being operably linked to a drug-regulatable promoter; and altering the concentration of regulatory drug to which the cell is exposed.