Abstract: This invention describes a design for a multiwell plate that contains integrated pumps that are used to stir each well of the plate. The device employs microfluidic logic technology to drive each peristaltic pump. This enables the plates to run autonomously, requiring only a static vacuum supply for power. The devices are entirely constructed out of low-cost polymers, with no electronics, and yet contains simple digital logic circuits to control the pumps. A stack of these plates may be run continuously in a standard cell culture incubator, allowing high-throughput culture of organoids.

Abstract: This invention describes a design for a multiwell plate that contains integrated pumps that are used to stir each well of the plate. The device employs microfluidic logic technology to drive each peristaltic pump. This enables the plates to run autonomously, requiring only a static vacuum supply for power. The devices are entirely constructed out of low-cost polymers, with no electronics, and yet contains simple digital logic circuits to control the pumps. A stack of these plates may be run continuously in a standard cell culture incubator, allowing high-throughput culture of organoids.

Abstract: Pneumatic devices for implementing finite state machines are provided. In some implementations, the pneumatic device comprises a state register component configured to hold one of a set of possible states. The pneumatic device also comprises a next-state logic block component configured to determine a next state for the state register component based at least in part on a current state of the state register component. A pneumatic programmable logic array (PLA) implementing a next state logic block of a finite state machine is also provided. The pneumatic PLA comprises an elastomeric membrane containing a pattern of holes and disposed between two channel layers of a pneumatic device. The PLA receives one or more input values representing a current state of a state register and one or more input values representing a user input and calculates one or more output values representing a next state for the state register.

Abstract: Pneumatic devices for implementing finite state machines are provided. In some implementations, the pneumatic device comprises a state register component configured to hold one of a set of possible states. The pneumatic device also comprises a next-state logic block component configured to determine a next state for the state register component based at least in part on a current state of the state register component. A pneumatic programmable logic array (PLA) implementing a next state logic block of a finite state machine is also provided. The pneumatic PLA comprises an elastomeric membrane containing a pattern of holes and disposed between two channel layers of a pneumatic device. The PLA receives one or more input values representing a current state of a state register and one or more input values representing a user input and calculates one or more output values representing a next state for the state register.