Abstract: The present invention relates to DNA constructs comprising a novel TIS sequence. The TIS sequence transcribes into an RNA motif that functions as the protein translation initiation site in an mRNA transcript. The DNA construct may further comprise a nucleic acid sequence encoding signal peptide. Additionally, the DNA constructs may also comprise a nucleic acid sequence encoding a recombinant protein or one or more polypeptide chains thereof. The invention further relates to an expression vector, expression cassette and host cell which comprise said DNA constructs. Furthermore, the present invention relates to a recombinant protein expressed by the host cell as well as a method for expressing the recombinant protein.

Abstract: A method for preparing a sterilised drug delivery device (1) comprising a fluid medication, the method comprising the steps of: providing a prefilled barrel (10) containing the fluid medication, the barrel being sealed with a stopper; placing the prefilled barrel, a plunger rod (20) and a finger grip (30) in a package such that the prefilled barrel, the plunger rod and the finger grip are separated from each other, wherein one side of the package is formed by a gas-permeable sheet (40); closing and sealing the package; and exposing the sealed package to a sterilant gas under conditions such that external surfaces of the prefilled barrel, the plunger rod and the finger grip are sterilised, wherein the package is a tray (50) comprising a cavity (51) formed therein wherein the cavity comprises at least three separate compartments (51a, 51b, 51c) adapted to the respective shapes of the components of the device for placement therein, and wherein the each of the prefilled barrel, the plunger rod and the finger grip

Abstract: The present invention relates to DNA construct suitable for expressing a recombinant protein in a bacterial host cell wherein said DNA construct comprises nucleotide sequences encoding: a rhaBAD promoter, a RhaR transcription activator, a RhaS transcription activator, an antibiotic resistance marker, a promoter operably linked to the nucleic acid encoding for the antibiotic resistance marker, an rmB T1 terminator, an rmB T2 terminator and a pMB1 origin of replication. The DNA construct may also comprise a nucleotide sequence encoding said recombinant protein operably linked to the rhaBAD promoter. The present invention further relates to a vector and bacterial host cell comprising said DNA construct as well as a method of producing said recombinant protein by exposing said bacterial host cell to rhamnose and thereby inducing expression of said recombinant protein.

Abstract: The present invention relates to DNA constructs comprising a novel TIS sequence. The TIS sequence transcribes into an RNA motif that functions as the protein translation initiation site in an mRNA transcript. The DNA construct may further comprise a nucleic acid sequence encoding signal peptide. Additionally, the DNA constructs may also comprise a nucleic acid sequence encoding a recombinant protein or one or more polypeptide chains thereof. The invention further relates to an expression vector, expression cassette and host cell which comprise said DNA constructs. Furthermore, the present invention relates to a recombinant protein expressed by the host cell as well as a method for expressing the recombinant protein.