Abstract: A nonenzymatic biosensor based on a metal-modified porous boron-doped diamond electrode, and a method for preparing the same and use thereof are provided. A working electrode of the nonenzymatic biosensor is a metal-modified porous boron-doped diamond electrode including a silicon wafer substrate and an electrode working layer arranged on a surface thereof, the electrode working layer is a porous boron-doped diamond layer modified with metal nanoparticles, and a pore surface of the porous boron-doped diamond layer contains an sp2 phase. In the present invention, by combining chemical vapor deposition and magnetron sputtering and by means of a tubular atmosphere annealing furnace and an electrochemical workstation, the preparation of a multi-metal-modified porous boron-doped diamond composite electrode is realized.