Abstract: The present invention provides a seminal plasma complex quality control serum, which is freeze-dried powders prepared by mixing a quality control serum preservation solution and a seminal plasma stock solution; wherein the quality control serum preservation solution is an aqueous solution containing 0.47% to 0.67% saccharides, 0.07% to 0.17% salts and 0.63% to 0.83% enzyme stabilizers; the seminal plasma stock solution is the seminal plasma obtained after the direct centrifugation of completely liquefied fresh human semen; and a mixing ratio by volume of the quality control serum preservation solution to the seminal plasma stock solution is 1:100 to 100:1. According to the present invention, a specific quality control serum preservation solution is used, then mixed with the seminal plasma at a suitable ratio by volume and finally subjected to freeze-drying treatment.