Patents by Inventor Sierd Bron
Sierd Bron has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7897742Abstract: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.Type: GrantFiled: June 1, 2007Date of Patent: March 1, 2011Assignee: Danisco US Inc.Inventors: Sierd Bron, Jan D. H. Jongbloed, Joerg Muller, Jan M. Van Dijl
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Patent number: 7884192Abstract: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.Type: GrantFiled: June 1, 2007Date of Patent: February 8, 2011Assignee: Danisco US Inc.Inventors: Sierd Bron, Jan D. H. Jongbloed, Joerg Muller, Jan M. Van Dijl
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Patent number: 7884191Abstract: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.Type: GrantFiled: June 1, 2007Date of Patent: February 8, 2011Assignee: Danisco US Inc.Inventors: Sierd Bron, Jan D. H. Jongbloed, Joerg Muller, Jan M. Van Dijl
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Patent number: 7553637Abstract: The present invention provides methods and compositions for the efficient and enhanced secretion of a protein of interest from a host cell. In specific, proteins are secreted through the Sec-dependent pathway, involving the spoIIIJ and/or yqjG gene product(s). In some embodiments, expression of the spoIIIJ and/or yqjG gene product(s) is modulated by a promoter operably linked to the gene.Type: GrantFiled: December 12, 2002Date of Patent: June 30, 2009Assignee: Danisco US Inc., Genencor DivisionInventors: Sierd Bron, Harold Tjalsma, Jan M. Van Dijl
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Publication number: 20090093025Abstract: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.Type: ApplicationFiled: June 1, 2007Publication date: April 9, 2009Inventors: Sierd Bron, Jan D.H. Jongbloed, Joerg Muller, Jan M. Van Dijl
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Publication number: 20080248525Abstract: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coil or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.Type: ApplicationFiled: June 1, 2007Publication date: October 9, 2008Inventors: Sierd Bron, Jan D.H. Jongbloed, Joerg Muller, Jan M. Van Dijl
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Publication number: 20080166757Abstract: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.Type: ApplicationFiled: June 1, 2007Publication date: July 10, 2008Inventors: Sierd Bron, Jan D.H. Jongbloed, Joerg Muller, Jan M. Van Dijl
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Patent number: 7316924Abstract: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.Type: GrantFiled: September 17, 2001Date of Patent: January 8, 2008Assignee: Genencor International, Inc.Inventors: Sierd Bron, Jan D. H. Jongbloed, Joerg Muller, Jan M. Van Dijl
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Publication number: 20050170511Abstract: The present invention provides methods and compositions for the efficient and enhanced secretion of a protein of interest from a host cell. In specific, proteins are secreted through the Sec-dependent pathway, involving the spoIIIJ and/or yqjG gene product(s). In some embodiments, expression of the spoIIIJ and/or yqjG gene product(s) is modulated by a promoter operably linked to the gene.Type: ApplicationFiled: December 12, 2002Publication date: August 4, 2005Inventors: Sierd Bron, Harold Tjalsma, Jan Van Dijl
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Publication number: 20020110860Abstract: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.Type: ApplicationFiled: September 17, 2001Publication date: August 15, 2002Inventors: Sierd Bron, Jan D.H. Jongbloed, Joerg Muller, Jan M. Van Dijl
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Patent number: 5246838Abstract: The subject invention describes the cloning and overexpression of leader peptidase genes. A method for isolating a leader peptidase gene is disclosed. Overexpression of the signal peptidase in a suitable host species leads to an enhanced rate of protein processing.Type: GrantFiled: February 28, 1991Date of Patent: September 21, 1993Assignee: Gist-brocades, N.V.Inventors: Jan M. Van Dijl, Hilde E. Smith, Sierd Bron, Wilhelmus J. Quax, Gerard Venema
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Patent number: 5212070Abstract: Novel vectors are provided for identifying secretory signal sequences from DNA fragments of unicellular microorganisms. The plasmids comprise a multiple cloning site with restriction sites in reading frame with a structural gene which permits rapid screening of the secreted expression product. Optionally, the vectors may include a promoter region upstream from the multiple cloning site. The invention is exemplified with Bacillus. Specific secretory signal sequences have been isolated with those vectors, allowing for efficient secretion into the supernatant, and not just to the periplasmic space to provide proteins in economically high yields. Secretory sequences are provided superior to other previously known sequences.Type: GrantFiled: December 13, 1990Date of Patent: May 18, 1993Assignee: Gist-brocadesInventors: Hilde E. Smith, Jan H. Van Ee, Ben P. H. Peeters, Sierd Bron, Gerard Venema
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Patent number: 5037760Abstract: Novel vectors are provided for identifying secretory signal sequences from DNA fragments of unicellular microorganisms. The plasmids comprise a multiple cloning site with restriction sites in reading frame with a structural gene which permits rapid screening of the screted expression product. Optionally, the vectors may include a promoter region upstream from the multiple cloning site. The invention is exemplified with Bacillus. Specific secretory signal sequence have been isolated with those vectors, allowing for efficient secretion into the supernatant, and not just to the periplasmic space to provide proteins in economically high yields. Secretory sequences are provided superior to other previously known sequences.Type: GrantFiled: May 1, 1987Date of Patent: August 6, 1991Assignee: Gist-Brocades NVInventors: Hilde E. Smith, Jan H. Van Ee, Ben P. H. Peeters, Sierd Bron, Gerard Venema