Abstract: The present invention relates to a method for constructing a chromatography test strip for triazophos based on molecular imprinting and electrospinning. The present invention combines electrospinning, molecular imprinting and the immunochromatography test strip technology. Molecularly imprinted T-line (detection limit) is prepared on an NC membrane by electrospinning, and goat anti-mouse IgG is used as C-line (quality control line). With fluorescence changes occurring when triazophos hapten-murine IgG/fluorescein isothiocyanate conjugate (THBu-IgG-FITC) fluorescent probe directly competes with the target triazophos to bind to the molecularly imprinted binding site, a chromatography-fluorescence detection method for triazophos based on molecular imprinting and electrospinning is established. The functional material adsorbing triazophos provided by the present invention adopts a virtual template to avoid template leakage, and can be used in immunochromatography to replace a biological antibody.