Abstract: Techniques are described for a computing system to maintain security by (a) detecting updates from a plurality of interrelated systems; (b) with reference to the detected updates and a set of mappings, determining an at-risk account having an improper access level; and (c) in response to determining the at-risk account, providing an alert of the at-risk account to an entity authorized to alter or remove the at-risk account. A system, apparatus, and computer program product for performing this method and similar methods are also described.

Abstract: Provided is a method for identifying balanced translocation breakpoints and a carrying state for balanced translocations in embryos, comprising the following steps: amplifying and sequencing a sample; comparing the sequence which is obtained by means of sequencing with a reference genome and analyzing copy numbers; accurately determining the position of a translocation breakpoint; detecting single nucleotide polymorphisms (SNPs) around the breakpoint and genotyping the SNPs; analyzing an embryonic haplotype, and comprehensively determining a normal chromosome and a translocation chromosome haplotype; determining the embryonic carrying state and, according to the haplotype, selecting an embryo which does not carry a balanced translocation.

Abstract: The present invention provides a method for determining endometrial receptivity and an application thereof. Specifically, the present invention provides a method for determining endometrial receptivity and markers for determining an endometrial receptivity status. The markers of the present invention could greatly reduce the error rate during the determination of the endometrial receptivity.

Abstract: The invention provides an analysis method and a device for determining a haplotype of a descendant object. Particularly, the invention provides a data analysis method for determining a haplotype genetic flow, comprising the following steps: (a) providing data sets for the analysis, the data sets being data sets related to genome information; (b) performing molecular marker genotyping in the upstream and downstream regions of Y1 target sites in each of the data sets, thereby obtaining molecular marker genotyping data, wherein Y1 is a positive integer greater than or equal to 1; (c) constructing a binary genetic vector of (0, 1) for each molecular marker site upstream and downstream of each target site in each of the data sets; (d) determining a maximum likelihood estimation value L using a Hidden Markov model for each target site; (e) determining a haplotype genetic flow direction of the descendant object and the family members through a Viterbi dynamic programming algorithm.

Abstract: The invention provides a method and a product for detecting the health status of an embryo by using a blastocyst culture medium. Specifically, the present invention provides an in vitro method for detecting the health status of embryos using blastocyst culture medium, characterized in that it comprises the steps of: (a) providing a first blastocyst culture system containing a blastocyst of in vitro culture on Day 5 (D5) to Day 6 (D6); (b) transferring the blastocysts into a second blastocyst culture system containing a fresh blastocyst culture medium, and culturing in the exchanged medium for a time of T1, thereby obtaining a spent blastocyst culture medium; (c) collecting the spent blastocyst culture medium, thereby obtaining a cell-free spent blastocyst culture medium, i.e. a test sample; and (d) performing a genetic assay to identify the health status of the blastocyst.

Abstract: The present application relates to a method of amplifying genomic DNA of a cell, comprising: (a) providing a reaction mixture, wherein the reaction mixture comprises the genomic DNA, a first type of primer, a second type of primer, a mixture of nucleotide monomers, and a nucleic acid polymerase, wherein the first type of primer comprises, in a 5? to 3? orientation, a common sequence and a variable sequence, wherein the common sequence consists of three or two types of bases selected from the group consisting of four types of bases: G, A, C and T, providing that the common sequence does not comprise G and C at the same time, and wherein the second type of primer comprises the common sequence but not the variable sequence; (b) placing the reaction mixture in a first thermal cycle program such that the variable sequence of the first type of primer can pair with the genomic DNA and amplify the genomic DNA to obtain a genomic amplification product, wherein the genomic amplification product comprises the common seq

Abstract: Provided is a method for identifying balanced translocation breakpoints and a carrying state for balanced translocations in embryos, comprising the following steps: amplifying and sequencing a sample; comparing the sequence which is obtained by means of sequencing with a reference genome and analyzing copy numbers; accurately determining the position of a translocation breakpoint; detecting single nucleotide polymorphisms (SNPs) around the breakpoint and genotyping the SNPs; analyzing an embryonic haplotype, and comprehensively determining a normal chromosome and a translocation chromosome haplotype; determining the embryonic carrying state and, according to the haplotype, selecting an embryo which does not carry a balanced translocation.

Abstract: The present application relates to a method of amplifying genomic DNA, comprising: (a) providing a first reaction mixture, wherein said first reaction mixture comprises a sample containing genomic DNA, a first primer, a mixture of nucleotide monomers, and a nucleic acid polymerase, wherein the first primer comprises, in a 5? to 3? orientation, a common sequence and a first variable sequence comprising a first random sequence; (b) placing the first reaction mixture in a first thermal cycle program to obtain a pre-amplification product; (c) providing a second reaction mixture, wherein said second reaction mixture comprises a pre-amplification product, a second primer, a mixture of nucleotide monomers, and a nucleic acid polymerase, wherein the second primer comprises or consists of, in a 5? to 3? orientation, a specific sequence and the common sequence; (d) placing the second reaction mixture in a second thermal cycle program for amplification, to obtain an amplification product.

Abstract: Provided is a method of detecting a chromosome abnormality in an embryo by using blastocyst culture. The method comprises: detecting embryonic circulating cell-free DNA in early embryonic in-vitro culture, i.e., blastocyst culture, performing uniform whole genome amplification on trace DNA, and then using a method, such as next generation sequencing, to perform analysis on the amplified DNA product, so as to determine a chromosome condition of an embryo, namely, whether aneuploidy or partial aneuploidy of chormosomes occurs.

Abstract: The present application relates to a method of amplifying genomic DNA of a cell, comprising: (a) providing a reaction mixture, wherein the reaction mixture comprises the genomic DNA, a first type of primer, a second type of primer, a mixture of nucleotide monomers, and a nucleic acid polymerase, wherein the first type of primer comprises, in a 5? to 3? orientation, a common sequence and a variable sequence, wherein the common sequence consists of three or two types of bases selected from the group consisting of four types of bases: G, A, C and T, providing that the common sequence does not comprise G and C at the same time, and wherein the second type of primer comprises the common sequence but not the variable sequence; (b) placing the reaction mixture in a first thermal cycle program such that the variable sequence of the first type of primer can pair with the genomic DNA and amplify the genomic DNA to obtain a genomic amplification product, wherein the genomic amplification product comprises the common seq

Abstract: Methods and compositions for amplifying the whole genome of a single cell are provided.

Abstract: Methods and compositions for amplifying the whole genome of a single cell are provided.

Abstract: A microscopy imaging system is disclosed that includes a light source system, focusing optics, an optical detector and a processor. The light source system is for providing an excitation beam at a center optical frequency ?e and for providing a stimulation beam at a center optical frequency ?s. The focusing optics is for directing and focusing the excitation beam toward a common focal volume such that a sample may be excited to an electronic excited state, and for directing and focusing the stimulation beam toward the common focal volume such that stimulated emission induced from the electronic excited state results in an increase in intensity of the stimulated beam. The optical detector is for detecting an increase in a radiation field at the center optical frequency ?s from stimulated emission from the common focal volume and for providing a detector signal. The processor is for receiving the detector signal and for providing a pixel of an image for the microscopy imaging system.