Patents by Inventor Silke Lutz
Silke Lutz has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9737484Abstract: A serum-stable mixture of lipids capable of encapsulating an active agent to form a liposome, said mixture comprising phosphatidylcholine and phosphatidylethanolamine in a ratio in the range of about 0.5 to about 8. The mixture may also include pH sensitive anionic and cationic amphiphiles, such that the mixture is amphoteric, being negatively charged or neutral at pH 7.4 and positively charged at pH 4. Amphoteric liposomes comprising such a mixture may be used for encapsulating nucleic acid therapeutics, such as oligonucleotides and DNA plasmids. The drug/lipid ratio may be adjusted to target the liposomes to particular organs or other sites in the body.Type: GrantFiled: November 12, 2014Date of Patent: August 22, 2017Assignee: Marina Biotech, Inc.Inventors: Steffen Panzner, Yvonne Kerwitz, Una Rauchhaus, Silke Lutz, Gerold Endert
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Publication number: 20160151282Abstract: An amphoteric liposome composed of a mixture of lipids, said mixture comprising a cationic amphiphile, an anionic amphiphile and optionally one or more neutral amphiphiles, at least one of said cationic and anionic amphiphiles being chargeable and the respective amounts of said cationic and anionic amphiphiles being selected such there is a stoichiometric excess of positively charged cationic amphiphile at a first lower pH, a stoichiometric excess of negatively charged anionic amphiphile at a second higher pH and said mixture has an isoelectric point intermediate said first and second pHs; characterised in that said positively charged cationic and negatively charged anionic amphiphiles are adapted to form a lipid salt with one another at said isoelectric point. Also disclosed are methods of predicting the fusogenicity of an amphoteric liposome at a given pH, formulating an amphoteric liposome and loading an amphoteric liposome with a cargo moiety.Type: ApplicationFiled: January 31, 2016Publication date: June 2, 2016Inventors: Steffen Panzner, Silke Lutz, Evgenios Siepi, Claudia Muller
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Patent number: 9283186Abstract: An amphoteric liposome composed of a mixture of lipids, said mixture comprising a cationic amphiphile, an anionic amphiphile and optionally one or more neutral amphiphiles, at least one of said cationic and anionic amphiphiles being chargeable and the respective amounts of said cationic and anionic amphiphiles being selected such there is a stoichiometric excess of positively charged cationic amphiphile at a first lower pH, a stoichiometric excess of negatively charged anionic amphiphile at a second higher pH and said mixture has an isoelectric point intermediate said first and second pHs; characterised in that said positively charged cationic and negatively charged anionic amphiphiles are adapted to form a lipid salt with one another at said isoelectric point. Also disclosed are methods of predicting the fusogenicity of an amphoteric liposome at a given pH, formulating an amphoteric liposome and loading an amphoteric liposome with a cargo moiety.Type: GrantFiled: January 13, 2012Date of Patent: March 15, 2016Assignee: Marina Biotech, Inc.Inventors: Steffen Panzner, Silke Lutz, Evgenios Siepi, Claudia Müller
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Publication number: 20150231073Abstract: A serum-stable mixture of lipids capable of encapsulating an active agent to form a liposome, said mixture comprising phosphatidylcholine and phosphatidylethanolamine in a ratio in the range of about 0.5 to about 8. The mixture may also include pH sensitive anionic and cationic amphiphiles, such that the mixture is amphoteric, being negatively charged or neutral at pH 7.4 and positively charged at pH 4. Amphoteric liposomes comprising such a mixture may be used for encapsulating nucleic acid therapeutics, such as oligonucleotides and DNA plasmids. The drug/lipid ratio may be adjusted to target the liposomes to particular organs or other sites in the body.Type: ApplicationFiled: November 12, 2014Publication date: August 20, 2015Inventors: Steffen Panzner, Yvonne Kerwitz, Una Rauchhaus, Silke Lutz, Gerold Endert
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Patent number: 9066867Abstract: A serum-stable mixture of lipids capable of encapsulating an active agent to form a liposome, said mixture comprising phosphatidylcholine and phosphatidylethanolamine in a ratio in the range of about 0.5 to about 8. The mixture may also include pH sensitive anionic and cationic amphiphiles, such that the mixture is amphoteric, being negatively charged or neutral at pH 7.4 and positively charged at pH 4. Amphoteric liposomes comprising such a mixture may be used for encapsulating nucleic acid therapeutics, such as oligonucleotides and DNA plasmids. The drug/lipid ratio may be adjusted to target the liposomes to particular organs or other sites in the body.Type: GrantFiled: September 9, 2010Date of Patent: June 30, 2015Assignee: Marina Biotech, Inc.Inventors: Steffen Panzner, Yvonne Kerwitz, Una Rauchhaus, Silke Lutz, Gerold Endert
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Publication number: 20140178462Abstract: An amphoteric liposome comprising neutral lipids wherein said neutral lipids are selected from the group comprising cholesterol or mixtures of cholesterol and at least one neutral or zwitterionic lipid and wherein K (neutral) of said mixtures is 0.3 or less. Said amphoteric liposome may encapsulate an active agent, such as nucleic acid therapeutics. Also disclosed are pharmaceutical compositions comprising said amphoteric liposomes as a carrier for the delivery or targeted delivery of active agents or ingredients.Type: ApplicationFiled: October 9, 2013Publication date: June 26, 2014Applicant: Marina Biotech, Inc.Inventors: Steffen Panzner, Silke Lutz, Evgenios Siepi, Claudia Muller, Ute Vinzens
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Patent number: 8193246Abstract: Lipid assemblies, such as liposomes, comprising transfection enhancer elements (TEE's), which are complexed with the lipid assemblies by means of ionic interactions, or lipids incorporating such TEE's are disclosed for enhancing the fusogenicity of the lipid assemblies. The TEE's have the formula: hydrophobic moiety-pH sensitive hydrophilic moiety??(II) The pH sensitive hydrophilic moiety of each TEE is a weak acid having a pka of between 2 and 6 or a zwitterionic structure comprising a combination of acidic groups with weak bases having a pKa of between 3 and 8. Lipids incorporating one or more such TEEs have the formula (I): Lipid moiety-[Hydrophobic moiety-pH sensitive hydrophilic moiety]??(I).Type: GrantFiled: December 19, 2007Date of Patent: June 5, 2012Assignee: Marina Biotech, Inc.Inventors: Steffen Panzner, Evgenios Siepi, Silke Lutz, Christian Reinsch, Claudia Müller
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Publication number: 20120135065Abstract: An amphoteric liposome composed of a mixture of lipids, said mixture comprising a cationic amphiphile, an anionic amphiphile and optionally one or more neutral amphiphiles, at least one of said cationic and anionic amphiphiles being chargeable and the respective amounts of said cationic and anionic amphiphiles being selected such there is a stoichiometric excess of positively charged cationic amphiphile at a first lower pH, a stoichiometric excess of negatively charged anionic amphiphile at a second higher pH and said mixture has an isoelectric point intermediate said first and second pHs; characterised in that said positively charged cationic and negatively charged anionic amphiphiles are adapted to form a lipid salt with one another at said isoelectric point. Also disclosed are methods of predicting the fusogenicity of an amphoteric liposome at a given pH, formulating an amphoteric liposome and loading an amphoteric liposome with a cargo moiety.Type: ApplicationFiled: January 13, 2012Publication date: May 31, 2012Inventors: Steffen Panzner, Silke Lutz, Evgenios Siepi, Claudia Müller
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Publication number: 20110076322Abstract: A serum-stable mixture of lipids capable of encapsulating an active agent to form a liposome, said mixture comprising phosphatidylcholine and phosphatidylethanolamine in a ratio in the range of about 0.5 to about 8. The mixture may also include pH sensitive anionic and cationic amphiphiles, such that the mixture is amphoteric, being negatively charged or neutral at pH 7.4 and positively charged at pH 4. Amphoteric liposomes comprising such a mixture may be used for encapsulating nucleic acid therapeutics, such as oligonucleotides and DNA plasmids. The drug/lipid ratio may be adjusted to target the liposomes to particular organs or other sites in the body.Type: ApplicationFiled: September 9, 2010Publication date: March 31, 2011Inventors: Steffen Panzner, Yvonne Kerwitz, Una Rauchhaus, Silke Lutz, Gerold Endert
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Publication number: 20100330154Abstract: An amphoteric liposome comprising neutral lipids wherein said neutral lipids are selected from the group comprising cholesterol or mixtures of cholesterol and at least one neutral or zwitterionic lipid and wherein K (neutral) of said mixtures is 0.3 or less. Said amphoteric liposome may encapsulate an active agent, such as nucleic acid therapeutics. Also disclosed are pharmaceutical compositions comprising said amphoteric liposomes as a carrier for the delivery or targeted delivery of active agents or ingredients.Type: ApplicationFiled: October 12, 2008Publication date: December 30, 2010Inventors: Steffen Panzner, Silke Lutz, Evgenios Siepi, Claudia Muller, Ute Vinzens
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Patent number: 7763275Abstract: A method for the production of nano- or microcapsules having a diameter of from 20 nm to 40 ?m is suggested, wherein template particles are supplied in an aqueous medium, electrically recharged with a polyelectrolyte, re-recharged without separation or washing steps using a second polyelectrolyte having a complementary charge with respect to the first polyelectrolyte, and said process is optionally continued with alternately charged polyelectrolytes.Type: GrantFiled: March 2, 2001Date of Patent: July 27, 2010Assignee: Novosom AGInventors: Steffen Panzner, Gerold Endert, Frank Essler, Anja Behrens, Silke Lutz, Cornelia Panzner
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Publication number: 20080306153Abstract: Lipid assemblies, such as liposomes, comprising transfection enhancer elements (TEE's), which are complexed with the lipid assemblies by means of ionic interactions, or lipids incorporating such TEE's are disclosed for enhancing the fusogenicity of the lipid assemblies. The TEE's have the formula: hydrophobic moiety-pH sensitive hydrophilic moiety??(II) The pH sensitive hydrophilic moiety of each TEE is a weak acid having a pka of between 2 and 6 or a zwitterionic structure comprising a combination of acidic groups with weak bases having a pKa of between 3 and 8.Type: ApplicationFiled: December 19, 2007Publication date: December 11, 2008Inventors: Steffen Panzner, Evgenios Siepi, Silke Lutz, Christian Reinsch, Claudia Muller
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Publication number: 20080089932Abstract: An amphoteric liposome composed of a mixture of lipids, said mixture comprising a cationic amphiphile, an anionic amphiphile and optionally one or more neutral amphiphiles, at least one of said cationic and anionic amphiphiles being chargeable and the respective amounts of said cationic and anionic amphiphiles being selected such there is a stoichiometric excess of positively charged cationic amphiphile at a first lower pH, a stoichiometric excess of negatively charged anionic amphiphile at a second higher pH and said mixture has an isoelectric point intermediate said first and second pHs; characterised in that said positively charged cationic and negatively charged anionic amphiphiles are adapted to form a lipid salt with one another at said isoelectric point. Also disclosed are methods of predicting the fusogenicity of an amphoteric liposome at a given pH, formulating an amphoteric liposome and loading an amphoteric liposome with a cargo moiety.Type: ApplicationFiled: October 12, 2007Publication date: April 17, 2008Inventors: Steffen Panzner, Silke Lutz, Evgenios Siepi, Claudia Muller
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Publication number: 20070104775Abstract: A serum-stable mixture of lipids capable of encapsulating an active agent to form a liposome, said mixture comprising phosphatidylcholine and phosphatidylethanolamine in a ratio in the range of about 0.5 to about 8. The mixture may also include pH sensitive anionic and cationic amphiphiles, such that the mixture is amphoteric, being negatively charged or neutral at pH 7.4 and positively charged at pH 4. Amphoteric liposomes comprising such a mixture may be used for encapsulating nucleic acid therapeutics, such as oligonucleotides and DNA plasmids. The drug/lipid ratio may be adjusted to target the liposomes to particular organs or other sites in the body.Type: ApplicationFiled: September 15, 2006Publication date: May 10, 2007Inventors: Steffen Panzner, Yvonne Kerwitz, Una Rauchhaus, Silke Lutz, Gerold Endert
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Publication number: 20070053918Abstract: The invention relates to formulations of liposomes and polymers for the production of an injectable depot of active substances, which has sustained release and effect in a mammal organism.Type: ApplicationFiled: May 10, 2004Publication date: March 8, 2007Applicant: NOVOSOM AGInventors: Steffen Panzner, Silke Lutz
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Publication number: 20060286161Abstract: The invention relates to liposomal formulations for the production of an injectable depot of peptide, protein and oligonucleotide active substances for sustained release and effect in a mammal body.Type: ApplicationFiled: May 8, 2004Publication date: December 21, 2006Inventors: Steffen Panzner, Silke Lutz
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Publication number: 20060159737Abstract: A pharmaceutical composition for local application is disclosed, said composition comprising a nucleic acid as a therapeutic agent, an excipient and a pharmaceutically acceptable vehicle therefor, said excipient comprising a liposome. The excipient comprises an amphoteric liposome having an isoelectric point between 4 and 7.4 and said composition is formulated to have a pH in the range 3 to 5. The composition may administered in the form of a colloidal suspension and may be buffered to the lower pH at the time of use by the addition of a suitable acidifying means to a substantially neutral suspension of the nucleic acid and excipient that may be more suitable for long-term storage of the composition. Alternatively, the composition may be lyophilised at the lower pH for subsequent reconstitution just prior to use with a suitable aqueous medium, such for example as substantially unbuffered water or saline.Type: ApplicationFiled: November 4, 2005Publication date: July 20, 2006Inventors: Steffen Panzner, Cornelia Panzner, Silke Lutz, Gerold Endert
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Publication number: 20030157181Abstract: A method for the production of nano- or microcapsules having a diameter of from 20 nm to 40 &mgr;m is suggested, wherein template particles are supplied in an aqueous medium, electrically recharged with a polyelectrolyte, re-recharged without separation or washing steps using a second polyelectrolyte having a complementary charge with respect to the first polyelectrolyte, and said process is optionally continued with alternately charged polyelectrolytes.Type: ApplicationFiled: September 3, 2002Publication date: August 21, 2003Inventors: Steffen Panzner, Gerold Endert, Frank Essler, Anja Behrens, Silke Lutz, Cornelia Panzner