Abstract: Hematopoietic stem cells (HSC) are essential to developing a functional immune system, including monocyte-macrophage cells required for wound healing. Described herein are methods of using yellow bone marrow as a source of cells to generate hematopoietic stem cells. The methods include expansion of human hematopoietic stem cells by culture of human yellow bone marrow in 3-dimensional hydrogel and pro-angiogenic, stem cell-promoting medium.

Abstract: Provided herein are methods and compositions for disrupting expression of nuclear receptor interacting protein 1 (Nrip1) in adipose cells. and methods of use of such adipose cells for treating, or reducing risk of, a condition associated with an elevated body mass index (BMI).

Abstract: Provided herein are methods and compositions for disrupting expression of nuclear receptor interacting protein 1 (Nrip1) in adipose cells, and methods of use of such adipose cells for treating, or reducing risk of, a condition associated with an elevated body mass index (BMI).

Abstract: Isolated hydrogel compositions derived from adipose tissue and related methods of preparation and use are provided. The isolated hydrogel compositions can include one or more of collagen 1A1, collagen 1A2, collagen 3A1, collagen 4A2, collagen 5A2, and fibrillin-1.

Abstract: Provided herein are methods and compositions for disrupting expression of nuclear receptor interacting protein 1 (Nrip1) in adipose cells, and methods of use of such adipose cells for treating, or reducing risk of, a condition associated with an elevated body mass index (BMI).

Abstract: Methods for preparation of prospectively identified human adipose stem cells enriched populations thereof, e.g., for therapy.

Abstract: Methods for preparation of human adipose stem cells, enriched populations thereof, and white adipose cells derived therefrom, for treatment of lipodystrophy.

Abstract: Methods for preparation of prospectively identified human adipose stem cells enriched populations thereof, e.g., for therapy.

Abstract: Methods for preparation of prospectively identified human adipose stem cells enriched populations thereof, e.g., for therapy.

Abstract: Methods for preparation of prospectively identified human adipose stem cells enriched populations thereof, e.g., for therapy.

Abstract: Methods for preparation of prospectively identified human adipose stem cells enriched populations thereof, e.g., for therapy.

Abstract: The invention features methods of enhancing whole body insulin sensitivity by modulating the endoplasmic reticulum (ER) stress response pathway in adipose cells. In one aspect, the methods involve targeting chaperone proteins important in the ER stress response pathway, for example, BiP and ERO-1. Another aspect features targeting the upstream transcription factors (e.g., CHOP-1 and XBP-1) of the genes encoding the chaperone proteins (and/or other ER proteins). Screening assays for identification of modulators of these ER proteins and/or transcription factors are also featured.

Abstract: Methods of identifying AMPK or AK modulators are provided. In particular, methods that feature identifying modulators of AK and its associated substrates (including AMP, ATP or AMP and ATP) or AMPK or activities associated therewith, are provided. Therapeutic methods utilizing compounds identified according to the methods of the invention are also provided.

Abstract: The invention features methods of enhancing whole body insulin sensitivity by modulating the endoplasmic reticulum (ER) stress response pathway in adipose cells. In one aspect, the methods involve targeting chaperone proteins important in the ER stress response pathway, for example, BiP and ERO-1. Another aspect features targeting the upstream transcription factors (e.g., CHOP-1 and XBP-1) of the genes encoding the chaperone proteins (and/or other ER proteins). Screening assays for identification of modulators of these ER proteins and/or transcription factors are also featured.

Abstract: The present invention relates, at least in part, to methods of modulating apoptosis in cells, comprising contacting the cell with a compound that modulates the expression, post-translational modification, or activity of Ian4, leucine-rich protein of 130 kD, GRP78, GRP94, or hsp60. The subject methods can be used to treat a variety of different disorders, for example, autoimmune disease and neoplasia. The invention also pertains to methods for identifying compounds that modulates the activity of Ian-4, leucine-rich protein of 130 kD, GRP78, GRP94, or hsp60.

Abstract: The invention relates to new lipid binding molecules that include a lipid binding domain and a reporter group. The new molecules can be used, for example, to detect and visualize lipids, such as phosphatidylinositol 3-phosphate.

Abstract: The invention discloses a chimeric GLUT transporter including a GLUT receptor polypeptide fused to a domain of a detectable heterologous polypeptide and cells expressing such reporter constructs. Cells expressing a detectably-tagged chimeric GLUT transporter are used in methods of screening candidate compounds for their ability to agonize or antagonize an interaction between a ligand and a receptor, e.g., insulin. In addition, a 30 amino acid intracellular retention sequence is disclosed.