Abstract: Microfluidic device (1) comprising at least one fluid channel (2) comprising at least one inlet (3), wherein said at least one fluid channel (2) is fluidly connected to a first sensor (4) downstream of said at least one fluid inlet (3), wherein the first sensor (4) comprises at least one sensor cathode (5) and at least one sensor anode (6) formed on an essentially electrically isolating substrate (7), wherein the sensor cathode (5) and the sensor anode (6) are spaced apart by a gap (8) formed on the electrically isolating substrate (7), and wherein at least one analyte capturing molecule (9) is immobilized in the gap (8) on the substrate (7), wherein the at least one capturing molecule (9) is adapted to capture at least one analyte (10) of a fluid sample introduced into the inlet (3) and transported to the first sensor (4).

Abstract: The present invention relates to a microfluidic device (1), preferably for producing a three-dimensional cell culture, having at least one chamber (2), and a fluid channel (3) which flows through at least part of the chamber (2) in order to provide a fluid stream which flows through the chamber (2) preferably continuously, wherein the chamber (2) is connected to a loading opening (4) and via the loading opening (4) can be loaded with hydrogel up to a desired fill level, characterized in that the chamber (2) comprises a main chamber (5) and a secondary chamber (6) connected to the main chamber (5), wherein, when the chamber (2) is being loaded with hydrogel up to the desired fill level, the secondary chamber is at least partially filled with hydrogel backed up from the main chamber (5).