Abstract: Various embodiments may increase scalability of image representations stored in a database for use in image matching and retrieval. For example, a system providing image matching can obtain images of a number of inventory items, extract features from each image using a feature extraction algorithm, and transform the same into their feature descriptor representations. These feature descriptor representations can be subsequently stored and used to compare against query images submitted by users. Though the size of each feature descriptor representation isn't particularly large, the total number of these descriptors requires a substantial amount of storage space. Accordingly, feature descriptor representations are compressed to minimize storage and, in one example, machine learning can be used to compensate for information lost as a result of the compression.

Abstract: System, including methods, apparatus, and compositions, for performing a multiplexed digital assay on a greater number of targets through combinatorial use of signals.

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

Abstract: Various embodiments may increase scalability of image representations stored in a database for use in image matching and retrieval. For example, a system providing image matching can obtain images of a number of inventory items, extract features from each image using a feature extraction algorithm, and transform the same into their feature descriptor representations. These feature descriptor representations can be subsequently stored and used to compare against query images submitted by users. Though the size of each feature descriptor representation isn't particularly large, the total number of these descriptors requires a substantial amount of storage space. Accordingly, feature descriptor representations are compressed to minimize storage and, in one example, machine learning can be used to compensate for information lost as a result of the compression.

Abstract: System, including methods, apparatus, and compositions, for performing a multiplexed digital assay on a greater number of targets through combinatorial use of signals.

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g., whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

Abstract: System, including methods and apparatus, for performing a digital assay with multiplexed detection of two or more distinct targets in the same optical channel.

Abstract: The present invention provides for determining relative copy number difference for one or more target nucleic acid sequences between a test sample and a reference sample or reference value derived therefrom. The methods facilitate the detection of copy number differences less than 1.5-fold.

Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

Abstract: System, including methods and apparatus, for performing a multiplexed digital assay.

Abstract: Various embodiments of the disclosed subject matter include computer-implemented methods, computer-readable media, and computer systems configured to respond to a search query from a computer user. In response to receiving a search query from the computer user, search results are obtained. A plurality of user intents are identified according to the obtained search results. The obtained search results are then grouped according to the subject matter of the search results, where each group corresponds to one of the plurality of user intents. A first user intent (with the corresponding group of search results) is selected. A search results page is generated from the search results of the group the search results corresponding to the selected first user intent and the generated search results page is returned to the computer user in response to the search query.

Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

Abstract: The present invention provides for determining relative copy number difference for one or more target nucleic acid sequences between a test sample and a reference sample or reference value derived therefrom. The methods facilitate the detection of copy number differences less than 1.5-fold.

Abstract: System, including methods and apparatus, for performing a digital assay with multiplexed detection of two or more distinct targets in the same optical channel.

Abstract: A system, including methods and apparatus, for performing a digital assay on a number of sample-containing replicates, each containing a plurality of sample-containing droplets, and measuring the concentration of target in the sample. Statistical meta-analysis techniques may be applied to reduce the effective variance of the measured target concentration.

Abstract: System, including methods, apparatus, and compositions, for performing a multiplexed digital assay on a greater number of targets through combinatorial use of signals.

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g., whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.