Patents by Inventor Simko Sama

Simko Sama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Uses of a saponin and method for its isolation
    Publication number: 20240285708
    Abstract: The present invention relates to the novel use of saponins comprising acetyl residues on one of their sugar residues. These saponins are able to enhance the transfection efficiency to a surprisingly much higher extent than already known saponins and even than Lipofectamin.
    Type: Application
    Filed: February 15, 2023
    Publication date: August 29, 2024
    Inventors: Alexander WENG, Matthias F. MELZIG, Simko SAMA
  • CYTOSOLIC DELIVERY OF GENOME EDITING TOOLS
    Publication number: 20240279684
    Abstract: A first aspect of the invention relates to the use of a saponin in the in vitro delivery of a nucleic acid into a cell. Typically, the nucleic acid is plasmid DNA, e.g. with a relatively large size of at least 5.5 kbp. In embodiments, the nucleic acid is transfected into cells in the presence of saponin GE1741 and/or saponin SO1861. A second aspect of the invention relates to a method for delivering a nucleic acid encoding for a CRISPR/Cas construct into a cell in vitro. Typically, the nucleic acid is plasmid DNA, e.g. with a relatively large size of at least 5.5 kbp. In embodiments, the nucleic acid is transfected into cells in the presence of saponin GE1741 and/or saponin SO1861. In embodiments, the nucleic acid is combined with poly-lysine, forming nanoplexes for transfection of the nucleic acid into a In cell. A third aspect of the invention relates to a kit of parts for delivering a nucleic acid encoding for a CRISPR/Cas construct into a cell in vitro.
    Type: Application
    Filed: May 19, 2022
    Publication date: August 22, 2024
    Inventors: Ruben POSTEL, Alexander WENG, Matthias Friedrich MELZIG, Simko SAMA
  • Uses of a saponin and method for its isolation
    Patent number: 11708388
    Abstract: The present invention relates to the novel use of saponins having acetyl residues on one of their sugar residues. These saponins are able to enhance the transfection efficiency to a surprisingly much higher extent than already known saponins and even than Lipofectamin.
    Type: Grant
    Filed: July 10, 2018
    Date of Patent: July 25, 2023
    Assignee: Freie Universität Berlin
    Inventors: Alexander Weng, Matthias F. Melzig, Simko Sama
  • EFFICIENT GENE DELIVERY TOOL WITH A WIDE THERAPEUTIC MARGIN
    Publication number: 20230039644
    Abstract: The disclosure relates to novel saponins comprising acetyl residues on two of their sugar residues. These saponins are able to enhance the transfection efficiency to a high extent and show much less cytotoxic side effects than already known saponins.
    Type: Application
    Filed: December 17, 2020
    Publication date: February 9, 2023
    Applicant: FREIE UNIVERSITÄT BERLIN
    Inventors: Simko SAMA, Alexander WENG, Hardy MITDANK
  • Novel uses of a saponin and method for its isolation
    Publication number: 20200131219
    Abstract: The present invention relates to the novel use of saponins having acetyl residues on one of their sugar residues. These saponins are able to enhance the transfection efficiency to a surprisingly much higher extent than already known saponins and even than Lipofectamin.
    Type: Application
    Filed: July 10, 2018
    Publication date: April 30, 2020
    Applicant: Freie Universität Berlin
    Inventors: Alexander Weng, Matthias F. Melzig, Simko Sama