Abstract: Provided herein compositions and methods including bioprinted tissue fibers comprising precisely patterned cancerous and non-cancerous cells that are capable of recapitulating a desired tumor microenvironment in vitro. The subject tissue models enable the automated high throughput screening of anti-cancer therapies in a biologically-relevant human system, and can be further personalized with patient-specific cells.

Abstract: Aspects of the disclosure include a pump assembly comprising a plurality of pumps (e.g. syringe pumps) positioned in a radial array on a mounting bracket. In embodiments, each syringe pump of the plurality of syringe pumps comprises a housing comprising a syringe retainer and a plunger driver. The pump assembly serves to minimize internal volume when used as part of a multi-channel microfluidic bioprinting system.

Abstract: A print head for a three-dimensional printer, which in one embodiment includes a multi-channel enclosure comprising a core channel outlet, a first shell channel outlet, and a first fluidic focusing chamber converging toward a dispensing channel, with the core channel outlet in a central region of the enclosure, and the core and shell channels extending a respective depth into the enclosure. In another embodiment a plurality of shell channels includes an inner shell channel extending a greater length into the focusing chamber than an outer shell channel, and a core channel extends a greater length into the focusing chamber than any shell channel. In another embodiment, each of the core and first shell channels includes at least two inlet sub-channels having distinct fluid reservoirs, input orifices and control valves, which converge to form a single outlet in communication with a respective focusing chamber. A sheath flow channel may be provided.

Abstract: Aspects of the disclosure include a fabrication platform for supporting a bioprinted fiber structures during printing, patterning, and/or processing, comprising a frame with a plurality of posts for securing a cross-linkable fiber during printing thereof, and where a continuous length of the cross-linkable fiber is printed around a plurality of posts during the 3D bioprinting process. The fabrication platform enables the cross-linkable fiber to be suspended during one or more of printing, patterning, and/or processing. In this way, the bioprinted fiber structure comprises a uniform outer surface, and can be easily modified and/or further processed after printing and patterning are completed.

Abstract: Provided herein are synthetic living tissue structures comprising multiple layers of fibers deposited in solidified form from a 3D bioprinter, together with kits and methods of use related thereto. The fibers comprise a plurality of mammalian cells dispensed within a solidified biocompatible matrix. The structural integrity of the fiber is maintained upon and after deposition without any additional crosslinking. The fiber is continuously bioprinted through at least two layers of the structure. In one aspect, synthetic muscle tissue structures exhibit a readily assayable contractile functionality.

Abstract: Aspects of the disclosure include an implantable composition/medical device encapsulating insulin-producing cells for use in the treatment and management of diabetes, generally comprising a multilayer lattice structure fabricated from a continuously bioprinted cell-laden core/shell fiber, the lattice structure having a defined infill density and at least coating applied to the lattice post-printing, and preferably at least one conformal coating. In this way, multilayer lattice structures are provided with improved stability, retrievability, and functionality.

Abstract: A print head, system and method for producing hollow fiber structures, for example three-dimensional biological structures comprising living cells, includes a dispensing channel, a core channel converging with the proximal end of the dispensing channel, a first shell channel converging with the core channel and the dispensing channel at a focusing intersection or chamber, and a sheath flow channel converging with the dispensing channel at a sheath flow intersection or chamber located between the focusing intersection or chamber and the distal end of the dispensing channel. The diameter of the dispensing channel increases from a first diameter to a second diameter at the sheath flow intersection or chamber, and the core channel has a third diameter less than the first and second diameters. The sheath flow channel includes sheath flow sub-channels and the focusing chamber has a conical frustum shape.

Abstract: Aspects of the invention include systems and methods for producing selectively permeable cell laden solid core fiber structures, and for producing three-dimensional (3D) biological structures for digital files. In embodiments, the printed fibers comprise living cells embedded in at least one annulus layer or the printed fibers comprise a solid core of at least one biological material. The tissue fiber can be used in tissue engineering as synthetic tissue structures.

Abstract: Microfluidic-based fiber formation methods and systems employ a computer vision and deep learning system and method to enable contactless sensing, analysis, and monitoring of key operational parameters within microfluidic crosslinking printheads on 3D bioprinters. Embodiments may employ object detection and/or semantic segmentation to facilitate the sensing, analysis, and monitoring. Deep learning can employ convolutional neural networks to localize and analyze the flow of different biological materials within the microchannels as well as to identify the operation of various microfluidic printhead on-chip components that can impact final quality of printed tissues. Printed tissues can include single-material fibers, including hollow filorezbers, as well as more complex coaxially-layered fibers.

Abstract: Provided herein are meniscus implant compositions, as well as methods for making and using the same. The subject meniscus implants find us in repairing and/or replacing damaged or diseased meniscal tissue in a mammalian subject.

Abstract: Aspects of the disclosure include a pump assembly comprising a plurality of pumps (e.g. syringe pumps) positioned in a radial array on a mounting bracket. In embodiments, each syringe pump of the plurality of syringe pumps comprises a housing comprising a syringe retainer and a plunger driver. The pump assembly serves to minimize internal volume when used as part of a multi-channel microfluidic bioprinting system.

Abstract: A print head, system and method for producing hollow fiber structures, for example three-dimensional biological structures comprising living cells, includes a dispensing channel, a core channel converging with the proximal end of the dispensing channel, a first shell channel converging with the core channel and the dispensing channel at a focusing intersection or chamber, and a sheath flow channel converging with the dispensing channel at a sheath flow intersection or chamber located between the focusing intersection or chamber and the distal end of the dispensing channel. The diameter of the dispensing channel increases from a first diameter to a second diameter at the sheath flow intersection or chamber, and the core channel has a third diameter less than the first and second diameters. The sheath flow channel includes sheath flow sub-channels and the focusing chamber has a conical frustum shape.

Abstract: Provided herein are meniscus implant compositions, as well as method for making and using the same. The subject meniscus implants find use in repairing and/or replacing damaged or diseased meniscal tissue in a mammalian subject.

Abstract: Provided herein are meniscus implant compositions, as well as methods for making and using the same. The subject meniscus implants find us in repairing and/or replacing damaged or diseased meniscal tissue in a mammalian subject.

Abstract: Provided herein are synthetic living tissue structures comprising multiple layers of fibers deposited in solidified form from a 3D bioprinter, together with kits and methods of use related thereto. The fibers comprise a plurality of mammalian cells dispensed within a solidified biocompatible matrix. The structural integrity of the fiber is maintained upon and after deposition without any additional crosslinking. The fiber is continuously bioprinted through at least two layers of the structure. In one aspect, synthetic muscle tissue structures exhibit a readily assayable contractile functionality.

Abstract: Provided herein are meniscus implant compositions, as well as method for making and using the same. The subject meniscus implants find use in repairing and/or replacing damaged or diseased meniscal tissue in a mammalian subject.

Abstract: Provided herein are synthetic living tissue structures comprising multiple layers of fibers deposited in solidified form from a 3D bioprinter, together with kits and methods of use related thereto. The fibers comprise a plurality of mammalian cells dispensed within a solidified biocompatible matrix. The structural integrity of the fiber is maintained upon and after deposition without any additional crosslinking. The fiber is continuously bioprinted through at least two layers of the structure. In one aspect, synthetic muscle tissue structures exhibit a readily assayable contractile functionality.

Abstract: Aspects of the invention include systems and methods for producing fiber structures, and for producing three-dimensional (3D) biological structures from digital files. In some embodiments, the printed fibers comprise living cells. Aspects of the invention further include a print head for producing the fiber structure, the print head comprising: a dispensing channel comprising a distal and proximal end; a dispensing orifice located at the distal end of the dispensing channel; one or more material channels converging with the dispensing channel at its proximal end; a buffer solution channel converging with the one or more material channels at the proximal end of the dispensing channel; and a sheath solution channel converging with the dispensing channel at a position located between the proximal and distal ends of the dispensing channel.

Abstract: A print head for a three-dimensional printer, which in one embodiment includes a multi-channel enclosure comprising a core channel outlet, a first shell channel outlet, and a first fluidic focusing chamber converging toward a dispensing channel, with the core channel outlet in a central region of the enclosure, and the core and shell channels extending a respective depth into the enclosure. In another embodiment a plurality of shell channels includes an inner shell channel extending a greater length into the focusing chamber than an outer shell channel, and a core channel extends a greater length into the focusing chamber than any shell channel. In another embodiment, each of the core and first shell channels includes at least two inlet sub-channels having distinct fluid reservoirs, input orifices and control valves, which converge to form a single outlet in communication with a respective focusing chamber. A sheath flow channel may be provided.

Abstract: A print head, system and method for producing hollow fiber structures, for example three-dimensional biological structures comprising living cells, includes a dispensing channel, a core channel converging with the proximal end of the dispensing channel, a first shell channel converging with the core channel and the dispensing channel at a focusing intersection or chamber, and a sheath flow channel converging with the dispensing channel at a sheath flow intersection or chamber located between the focusing intersection or chamber and the distal end of the dispensing channel. The diameter of the dispensing channel increases from a first diameter to a second diameter at the sheath flow intersection or chamber, and the core channel has a third diameter less than the first and second diameters. The sheath flow channel includes sheath flow sub-channels and the focusing chamber has a conical frustum shape.