Abstract: Provided herein are meniscus implant compositions, as well as methods for making and using the same. The subject meniscus implants find us in repairing and/or replacing damaged or diseased meniscal tissue in a mammalian subject.

Abstract: Provided herein are synthetic living tissue structures comprising multiple layers of fibers deposited in solidified form from a 3D bioprinter, together with kits and methods of use related thereto. The fibers comprise a plurality of mammalian cells dispensed within a solidified biocompatible matrix. The structural integrity of the fiber is maintained upon and after deposition without any additional crosslinking. The fiber is continuously bioprinted through at least two layers of the structure. In one aspect, synthetic muscle tissue structures exhibit a readily assayable contractile functionality.

Abstract: Provided herein are meniscus implant compositions, as well as method for making and using the same. The subject meniscus implants find use in repairing and/or replacing damaged or diseased meniscal tissue in a mammalian subject.

Abstract: Provided herein are synthetic living tissue structures comprising multiple layers of fibers deposited in solidified form from a 3D bioprinter, together with kits and methods of use related thereto. The fibers comprise a plurality of mammalian cells dispensed within a solidified biocompatible matrix. The structural integrity of the fiber is maintained upon and after deposition without any additional crosslinking. The fiber is continuously bioprinted through at least two layers of the structure. In one aspect, synthetic muscle tissue structures exhibit a readily assayable contractile functionality.

Abstract: Aspects of the invention include systems and methods for producing fiber structures, and for producing three-dimensional (3D) biological structures from digital files. In some embodiments, the printed fibers comprise living cells. Aspects of the invention further include a print head for producing the fiber structure, the print head comprising: a dispensing channel comprising a distal and proximal end; a dispensing orifice located at the distal end of the dispensing channel; one or more material channels converging with the dispensing channel at its proximal end; a buffer solution channel converging with the one or more material channels at the proximal end of the dispensing channel; and a sheath solution channel converging with the dispensing channel at a position located between the proximal and distal ends of the dispensing channel.

Abstract: A print head for a three-dimensional printer, which in one embodiment includes a multi-channel enclosure comprising a core channel outlet, a first shell channel outlet, and a first fluidic focusing chamber converging toward a dispensing channel, with the core channel outlet in a central region of the enclosure, and the core and shell channels extending a respective depth into the enclosure. In another embodiment a plurality of shell channels includes an inner shell channel extending a greater length into the focusing chamber than an outer shell channel, and a core channel extends a greater length into the focusing chamber than any shell channel. In another embodiment, each of the core and first shell channels includes at least two inlet sub-channels having distinct fluid reservoirs, input orifices and control valves, which converge to form a single outlet in communication with a respective focusing chamber. A sheath flow channel may be provided.

Abstract: A print head, system and method for producing hollow fiber structures, for example three-dimensional biological structures comprising living cells, includes a dispensing channel, a core channel converging with the proximal end of the dispensing channel, a first shell channel converging with the core channel and the dispensing channel at a focusing intersection or chamber, and a sheath flow channel converging with the dispensing channel at a sheath flow intersection or chamber located between the focusing intersection or chamber and the distal end of the dispensing channel. The diameter of the dispensing channel increases from a first diameter to a second diameter at the sheath flow intersection or chamber, and the core channel has a third diameter less than the first and second diameters. The sheath flow channel includes sheath flow sub-channels and the focusing chamber has a conical frustum shape.

Abstract: Provided herein are meniscus implant compositions, as well as method for making and using the same. The subject meniscus implants find use in repairing and/or replacing damaged or diseased meniscal tissue in a mammalian subject.

Abstract: Aspects of the invention include systems and methods for producing fiber structures, and for producing three-dimensional (3D) biological structures from digital files. In some embodiments, the printed fibers comprise living cells. Aspects of the invention further include a print head for producing the fiber structure, the print head comprising: a dispensing channel comprising a distal and proximal end; a dispensing orifice located at the distal end of the dispensing channel; one or more material channels converging with the dispensing channel at its proximal end; a buffer solution channel converging with the one or more material channels at the proximal end of the dispensing channel; and a sheath solution channel converging with the dispensing channel at a position located between the proximal and distal ends of the dispensing channel.

Abstract: Provided herein are meniscus implant compositions, as well as method for making and using the same. The subject meniscus implants find use in repairing and/or replacing damaged or diseased meniscal tissue in a mammalian subject.

Abstract: Provided herein are synthetic living tissue structures comprising multiple layers of fibers deposited in solidified form from a 3D bioprinter, together with kits and methods of use related thereto. The fibers comprise a plurality of mammalian cells dispensed within a solidified biocompatible matrix. The structural integrity of the fiber is maintained upon and after deposition without any additional crosslinking. The fiber is continuously bioprinted through at least two layers of the structure. In one aspect, synthetic muscle tissue structures exhibit a readily assayable contractile functionality.