Abstract: Disclosed are resins, resin-protein/peptide complexes and methods for purifying proteins and peptides using said resins. The resins described herein are useful for the binding of a selected protein or peptide, particularly from an aqueous medium such as a fermentation broth, by hydrophobic interactions between the resin and the selected protein or peptide. The resin is characterized by the fact that it contains ionizable ligands and/or functionalities which are uncharged at the pH of binding the target protein or peptide, thereby facilitating hydrophobic interactions, and charged at the pH of desorption, thereby disrupting the established hydrophobic interaction between the resin and the target protein or peptide. More particularly, the present invention is directed to the use of the described resins in the purification of recombinant enzyme products such as proteases, for example, chymosin or subtilisin.

Abstract: Disclosed are rationally designed mixed mode resins which are useful in recovering a target compound from an aqueous solution and methods for use of such resins. The resins described herein have a hydrophobic character at the pH of binding of the target compound and a hydrophilic and/or electrostatic character at the pH of desorption of the target compound from the resin and are specifically designed to bind the target compound from an aqueous solution at both a low and high ionic strength.

Abstract: Disclosed are methods for the preparation of resins comprising a support matrix having ligands capable of binding a target compound covalently attached thereto through a linking group comprising sulfide, sulfoxide, or sulfone functionality. Specifically, in the disclosed methods, a support matrix comprising ethylenically unsaturated functionality is converted to sulfide linkage by contact with a thiol group under free radical reaction conditions and subsequent optional oxidation of the sulfide to the sulfone or sulfoxide functionality.

Abstract: Disclosed are rationally designed mixed mode resins which are useful in recovering a target compound from an aqueous solution and methods for use of such resins. The resins described herein have a hydrophobic character at the pH of binding of the target compound and a hydrophilic and/or electrostatic character at the pH of desorption of the target compound from the resin and are specifically designed to bind the target compound from an aqueous solution at both a low and high ionic strength.