Abstract: A plant cell comprising: (a) a polynucleotide sequence encoding a nitrate reductase polypeptide comprising a contiguous polypeptide sequence of SEQ ID NO: 5 or SEQ ID NO: 7, wherein methionine is substituted for an amino acid that reduces nitrate reductase activity in the plant cell as compared to a control plant cell; (b) a polypeptide sequence encoded by the polynucleotide sequence set forth in (a); or (c) a construct, vector or expression vector comprising the polynucleotide sequence set forth in (b).

Abstract: An aerosol-generating substrate for a heated aerosol-generating article is provided, the aerosol-generating substrate including: homogenised tobacco material, the homogenised tobacco material including dried green tobacco material, an aerosol former, and a binder, the homogenised tobacco material having an aerosol former content of between 5 percent and 55 percent by weight on a dry weight basis. A dried green tobacco material for forming an aerosol-generating substrate for an aerosol-generating article, and a method of producing dried green tobacco material for the aerosol-generating substrate, are also provided.

Abstract: There is described herein a mutant, non-naturally occurring or transgenic plant or part thereof having reduced expression of the gene encoding Terminal Flower 1 (TFL1) or reduced activity of the protein encoded by TFL1, said TFL1 comprising, consisting or consisting essentially of (i) a polynucleotide sequence comprising, consisting or consisting essentially of a sequence having at least 72% sequence identity to SEQ ID NO:7 or SEQ ID NO:8 or SEQ ID NO:10 or SEQ ID NO:11 or SEQ ID NO:19 or SEQ ID NO:20; or (ii) a polypeptide encoded by the polynucleotide set forth in (i); or (iii) a polypeptide having at least 72% sequence identity to SEQ ID NO:9 or SEQ ID NO:12 or SEQ ID NO:21; wherein the expression or activity of the polynucleotide or the polypeptide set forth in (i), (ii) or (iii) is reduced as compared to a control plant in which the expression or activity of the polynucleotide or the polypeptide set forth in (i), (ii) or (iii) has not been reduced.

Abstract: A plant cell comprising: (a) a polynucleotide sequence encoding a nitrate reductase polypeptide comprising a contiguous polypeptide sequence of SEQ ID NO: 5 or SEQ ID NO: 7, wherein methionine is substituted for an amino acid that reduces nitrate reductase activity in the plant cell as compared to a control plant cell; (b) a polypeptide sequence encoded by the polynucleotide sequence set forth in (a); or (c) a construct, vector or expression vector comprising the polynucleotide sequence set forth in (b).

Abstract: There is described herein a mutant, non-naturally occurring or transgenic plant or part thereof having reduced expression of the gene encoding Terminal Flower 1 (TFL1) or reduced activity of the protein encoded by TFL1, said TFL1 comprising, consisting or consisting essentially of (i) a polynucleotide sequence comprising, consisting or consisting essentially of a sequence having at least 72% sequence identity to SEQ ID NO:7 or SEQ ID NO:8 or SEQ ID NO:10 or SEQ ID NO:11 or SEQ ID NO:19 or SEQ ID NO:20; or (ii) a polypeptide encoded by the polynucleotide set forth in (i); or (iii) a polypeptide having at least 72% sequence identity to SEQ ID NO:9 or SEQ ID NO:12 or SEQ ID NO:21; wherein the expression or activity of the polynucleotide or the polypeptide set forth in (i), (ii) or (iii) is reduced as compared to a control plant in which the expression or activity of the polynucleotide or the polypeptide set forth in (i), (ii) or (iii) has not been reduced.

Abstract: The present invention relates to a mutant, non-naturally occurring or transgenic tobacco plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a functional nicotine N-demethylase and having at least 95% sequence identity to SEQ ID NO:2; (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide comprising, consisting or consisting essentially of a sequence encoding a nicotine N-demethylase and having at least 95% sequence identity to SEQ ID NO:3; or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i), and wherein the expression or activity of said nicotine demethylase is reduced as compared to a control tobacco plant cell in which the expression or activity of said nicotine demethylase has not been reduced.

Abstract: The present invention relates to a mutant, non-naturally occurring or transgenic plant cell comprising: (i) at least one polynucleotide comprising, consisting or consisting essentially of a sequence encoding an isopropylmalate synthase and having at least 60% sequence identity to SEQ ID NO:1 or SEQ ID NO:10 or SEQ ID NO: 12 or SEQ ID NO:14; or (ii) a polypeptide encoded by said polynucleotide(s); or (iii) a polypeptide having at least 60% sequence identity to SEQ ID NO:2 or SEQ ID NO:11 or SEQ ID NO:13 or SEQ ID NO:15; or (iv) a construct, vector or expression vector comprising said polynucleotide sequence(s), optionally wherein said construct, vector or expression vector additionally comprises a promoter comprising, consisting or consisting essentially of the sequence set forth in SEQ ID NO:8 or a variant thereof with at least about 60% identity thereto or a trichome promoter.

Abstract: The present invention relates to a mutant, non-naturally occurring or transgenic tobacco plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a functional nicotine N-demethylase and having at least 95% sequence identity to SEQ ID NO:2; (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide comprising, consisting or consisting essentially of a sequence encoding a nicotine N-demethylase and having at least 95% sequence identity to SEQ ID NO:3; or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i), and wherein the expression or activity of said nicotine demethylase is reduced as compared to a control tobacco plant cell in which the expression or activity of said nicotine demethylase has not been reduced.

Abstract: The present invention relates to a mutant, non-naturally occurring or transgenic plant cell comprising: (i) at least one polynucleotide comprising, consisting or consisting essentially of a sequence encoding an isopropylmalate synthase and having at least 60% sequence identity to SEQ ID NO:1 or SEQ ID NO:10 or SEQ ID NO: 12 or SEQ ID NO:14; or (ii) a polypeptide encoded by said polynucleotide(s); or (iii) a polypeptide having at least 60% sequence identity to SEQ ID NO:2 or SEQ ID NO:11 or SEQ ID NO:13 or SEQ ID NO:15; or (iv) a construct, vector or expression vector comprising said polynucleotide sequence(s), optionally wherein said construct, vector or expression vector additionally comprises a promoter comprising, consisting or consisting essentially of the sequence set forth in SEQ ID NO:8 or a variant thereof with at least about 60% identity thereto or a trichome promoter.