Patents by Inventor Simon Lawrence John Stubbs

Simon Lawrence John Stubbs has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Fluorescent proteins and methods of using same
    Patent number: 7300762
    Abstract: The present invention provides novel engineered derivatives of green fluorescent protein (GFP) which have an amino acid sequence which is modified by amino acid substitution compared with the amino acid sequence of wild type Green Fluorescent Protein (SEQ ID NO: 2). The modified GFPs exhibit enhanced fluorescence relative to wtGFP when expressed in non-homologous cells at temperatures above 30° C., and when excited at about 490 nm compared to the parent proteins, i.e. wtGFP. An example of a preferred protein is F64L-S175G-E222G-GFP. The modified GFPs provide a means for detecting GFP reporters in mammalian cells at lower levels of expression and/or increased sensitivity relative to wtGFP. This greatly improves the usefulness of fluorescent proteins in studying cellular functions in living cells.
    Type: Grant
    Filed: October 14, 2005
    Date of Patent: November 27, 2007
    Assignee: GE Healthcare UK Limited
    Inventors: Simon Lawrence John Stubbs, Anne Elizabeth Jones, Nigel Paul Michael, Nicholas Thomas
  • Fluorescent proteins
    Patent number: 7091317
    Abstract: The present invention provides novel engineered derivatives of green fluorescent protein (GFP) which have an amino acid sequence which is modified by amino acid substitution compared with the amino acid sequence of wild type Green Fluorescent Protein. The modified GFPs exhibit enhanced fluorescence relative to wtGFP when expressed in non-homologous cells at temperatures above 30° C., and when excited at about 490 nm compared to the parent proteins, i.e. wtGFP. An example of a preferred protein is F64L-S175G-E222G-GFP. The modified GFPs provide a means for detecting GFP reporters in mammalian cells at lower levels of expression and/or increased sensitivity relative to wtGFP. This greatly improves the usefulness of fluorescent proteins in studying cellular functions in living cells.
    Type: Grant
    Filed: January 14, 2004
    Date of Patent: August 15, 2006
    Assignee: GE Healthcare Limited
    Inventors: Simon Lawrence John Stubbs, Anne Elizabeth Jones, Nigel Paul Michael, Nicholas Thomas
  • Fluorescent proteins
    Patent number: 6919186
    Abstract: The present invention provides novel engineered derivatives of green fluorescent protein (GFP) which have an amino acid sequence which is modified by amino acid substitution compared with the amino acid sequence of wild type Green Fluorescent Protein. The modified GFPs exhibit enhanced fluorescence relative to wtGFP when expressed in non-homologous cells at temperatures above 30° C., and when excited at about 490 nm compared to the parent proteins, i.e. wtGFP. An example of a preferred protein is F64L-S175G-E222G-GFP. The modified GFPs provide a means for detecting GFP reporters in mammalian cells at lower levels of expression and/or increased sensitivity relative to wtGFP. This greatly improves the usefulness of fluorescent proteins in studying cellular functions in living cells.
    Type: Grant
    Filed: September 28, 2001
    Date of Patent: July 19, 2005
    Assignee: Amersham plc
    Inventors: Simon Lawrence John Stubbs, Anne Elizabeth Jones, Nigel Paul Michael, Nicholas Thomas
  • Fluorescent proteins
    Publication number: 20040138420
    Abstract: The present invention provides novel engineered derivatives of green fluorescent protein (GFP) which have an amino acid sequence which is modified by amino acid substitution compared with the amino acid sequence of wild type Green Fluorescent Protein. The modified GFPs exhibit enhanced fluorescence relative to wtGFP when expressed in non-homologous cells at temperatures above 30° C., and when excited at about 490 nm compared to the parent proteins, i.e. wtGFP. An example of a preferred protein is F64L-S 175G-E222G-GFP. The modified GFPs provide a means for detecting GFP reporters in mammalian cells at lower levels of expression and/or increased sensitivity relative to wtGFP. This greatly improves the usefulness of fluorescent proteins in studying cellular functions in living cells.
    Type: Application
    Filed: January 14, 2004
    Publication date: July 15, 2004
    Inventors: Simon Lawrence John Stubbs, Anne Elizabeth Jones, Nigel Paul Michael, Nicholas Thomas
  • Fluorescent proteins
    Publication number: 20030175859
    Abstract: The present invention provides novel engineered derivatives of green fluorescent protein (GFP) which have an amino acid sequence which is modified by amino acid substitution compared with the amino acid sequence of wild type Green Fluorescent Protein. The modified GFPs exhibit enhanced fluorescence relative to wtGFP when expressed in non-homologous cells at temperatures above 30° C., and when excited at about 490 nm compared to the parent proteins, i.e. wtGFP. An example of a preferred protein is F64L-S175G-E222G-GFP. The modified GFPs provide a means for detecting GFP reporters in mammalian cells at lower levels of expression and/or increased sensitivity relative to wtGFP. This greatly improves the usefulness of fluorescent proteins in studying cellular functions in living cells.
    Type: Application
    Filed: September 28, 2001
    Publication date: September 18, 2003
    Inventors: Simon Lawrence John Stubbs, Anne Elizabeth Jones, Nigel Paul Michael, Nicholas Thomas