Abstract: The present disclosure relates to engineered acetate kinase enzymes and compositions thereof, recombinant polynucleotides encoding the engineered acetate kinase enzymes, and method of using the engineered acetate kinase enzymes.

Abstract: The present disclosure provides engineered adenylate kinases, and recombinant polynucleotides encoding the engineered adenylate kinases. The present disclosure further provides uses of the engineered adenylate kinases for converting NMP to NDP.

Abstract: The present disclosure provides engineered adenosine kinase polypeptides, and recombinant polynucleotides encoding the engineered adenosine kinases. The present disclosure further provides method of using the engineered adenosine kinases.

Abstract: Write apparatus for a disk drive includes a write head, write current circuitry connected to the write head for generating a steady-state write current, overshoot current circuitry connected to the write head for generating write current overshoot pulses, and a T-coil termination network between (a) the write current circuitry and the overshoot current circuitry, and (b) a first node connected to a first input of a transmission line together with the write head. The T-coil termination network may include a first inductor connected to the first node, a second inductor coupled with the first inductor at a second node, and a first termination resistor between the first inductor and a common voltage. An output of the overshoot current circuitry may be connected to the first node, and an output of the write current circuitry may be connected to the second node.

Abstract: The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity on a 1-tert-Butoxycarbonylaminocyclopentanoic acid substrate.

Abstract: The present disclosure provides enzymatic methods for the production of 3?-phosphate-nucleoside-5?-triphosphate (NQP). The present disclosure further provides enzymatic methods for the production of nucleotide-5?-triphosphates.

Abstract: The present application provides a method of quantifying an amount of a derivatized peptide displayed on a phage by phage display, the method comprising: providing a phage containing a target peptide thereon; reacting the phage containing the target peptide with a first reagent to derivatize the target peptide to form a derivatized peptide, reacting the derivatized peptide with a capture agent comprising a detection marker, thereby incorporating the detection marker within the derivatized peptide; and determining an amount of the detection marker, thereby quantifying the amount of the derivatized peptide dis-played on the phage. A kit comprising a capture agent compris-ing a detection marker for quantifying the phage displayed derivatized peptides is also provided.

Abstract: A peptide that binds to elongation initiation factor 4E (eIF4E) comprising the amino acid sequence CEX1GX2X3X4X5C (SEQ ID NO: 1), where X1 is an amino acid selected from the group consisting of threonine (T), methionine (M) or leucine (L), X2 and X3 is an amino acid selected from the group consisting of phenylalanine (F), modified phenylalanine and tyrosine (Y), X4 and X5 is any amino acid, wherein the two cysteine residues are joined by a disulphide bond. Further, pharmaceutical compositions and uses of the peptide, and pharmaceutical compositions are provided.

Abstract: The present invention provides a binding assay that employs an activatable dormant bacteriophage carrying a reporter gene to qualitatively or quantitatively detect the presence of a substance of interest in a sample. Also provided is a simple culture device that is designed for manufacture and use in areas of limited resources. The device is useful for cell culture in such environments with limited resources because cells grow in paper just as they do in a culture dish.

Abstract: The present invention provides methods for assessing the likelihood of effectiveness of chemotherapy such as oxaliplatin treatment in colorectal cancer patients as well as their survival prospect by determining the level of miR-133a in the cancer tissue. A kit useful for such methods are also provided.

Abstract: The present invention provides methods for assessing the likelihood of effectiveness of chemotherapy such as oxaliplatin treatment in colorectal cancer patients as well as their survival prospect by determining the level of miR-133a in the cancer tissue. A kit useful for such methods are also provided.

Abstract: The present application provides a method of synthesizing a genetically-encoded chemical modification of a peptide library. A vector in a substrate, such as a phage, is modified to include a peptide linker and a modification to form a genetic “barcode”. The barcode is screened against potential targets which may be used in drug discovery.

Abstract: Iron nanoparticles that are useful for constructing electrodes for lithium ion batteries and a method of making said particles is disclosed herein. The nanoparticles may include magnetite. The electrode may be constructed by centrifuging the nanoparticles to a current collector, such as a disc of copper, without the use of an extrinsic binder. The solvothermal method of making nanoparticles decreases the time of the procedure from about 24 hours to about 75 minutes. The method of making electrode decreases the complexity and number of steps compared to the conventional procedure to prepare an electrode, and eliminates the use of additives (binder and current enhancer) and toxic NMP solvents in the electrode preparation process.

Abstract: The present application provides a method of quantifying an amount of a derivatized peptide displayed on a phage by phage display, the method comprising: providing a phage containing a target peptide thereon; reacting the phage containing the target peptide with a first reagent to derivatize the target peptide to form a derivatized peptide, reacting the derivatized peptide with a capture agent comprising a detection marker, thereby incorporating the detection marker within the derivatized peptide; and determining an amount of the detection marker, thereby quantifying the amount of the derivatized peptide dis-played on the phage. A kit comprising a capture agent compris-ing a detection marker for quantifying the phage displayed derivatized peptides is also provided.

Abstract: This disclosure describes a new method and device that lead to improved performance (in the areas of energy density, power density, and cycle life) of any sulfur or selenide based including their respective polysulfide/polyselenides battery configurations both for stationary and portable applications. A device capable of converting lower polysulfides/polyselenides to higher polysulfides/polyselenides (and vice versa) and trapping said compounds in a highly porous matrix is described. The device may be rechargeable. Catalytically-active materials such as bulk metal structures, thin films of metals, microporous/nanoporous structures of materials, and carbon composites of metals and their alloys can be used as current collectors, electrodes, or both.

Abstract: The present application provides a method of quantifying an amount of a derivatized peptide displayed on a phage by phage display, the method comprising: providing a phage containing the target peptide thereon; reacting the phage containing the target peptide with a first reagent to derivatize the target peptide to form a derivatized peptide, reacting the derivatized peptide with a capture agent comprising a detection marker, thereby incorporating the detection marker within the derivatized peptide; and determining an amount of the detection marker, thereby quantifying the amount of the derivatized peptide displayed on the phage. A kit comprising a capture agent comprising a detection marker for quantifying the phage displayed derivatized peptides is also provided.

Abstract: A composite anode for a lithium-ion battery is manufactured from silicon nanoparticles having diameters mostly under 10 nm; providing an oxide layer on the silicon nanoparticles; dispersing the silicon nanoparticles in a polar liquid; providing a graphene oxide suspension; mixing the polar liquid containing the dispersed silicone nanoparticles with the graphene oxide suspension to obtain a composite mixture; probe-sonicating the mixture for a predetermined time; filtering the composite mixture to obtain a solid composite; drying the composite; and reducing the composite to obtain graphene and silicon.

Abstract: The present application provides a method of synthesizing a genetically-encoded chemical modification of a peptide library. A vector in a substrate, such as a phage, is modified to include a peptide linker and a modification to form a genetic “barcode”. The barcode is screened against potential targets which may be used in drug discovery.

Abstract: Materials and methods for converting brown grease to useful diesel fuel are described. One material is a palladium catalyst on a silicon/carbon support. A method comprises flowing fresh hydrogen over a reaction of diluted brown grease on a palladium/carbon catalyst.

Abstract: A catalyst comprises a carbide or nitride of a metal and a promoter element. The metal is selected from the group consisting of Mo, W, Co, Fe, Rh or Mn, and the promoter element is selected from the group consisting of Ni, Co, Al, Si, S or P, provided that the metal and the promoter element are different. The catalyst also comprises a mesoporous support having a surface area of at least about 170 m2 g?1, wherein the carbide or nitride of the metal and the promoter element is supported by the mesoporous support, and is in a non-sulfided form and in an amorphous form.