Abstract: A process for producing PHA comprises obtaining biomass produced in the course of biologically treating a first wastewater source containing RBCOD. The biomass is to be exploited with a second wastewater source having a different RBCOD content from the first wastewater source in order to accumulate and thereby produce PHA. Before subjecting the biomass to a PHA accumulation process, the biomass PHA accumulation potential is enhanced via an acclimation process with the second wastewater source. During acclimation, the biomass is subjected to repeated feast-famine periods. During each feast period, the biomass is exposed to a fraction of the second wastewater source. The RBCOD uptake and/or biomass respiration rate is directly or indirectly measured during each feast period. The famine period is maintained for a period of time that is at least two times greater than the length of time of the proceeding feast period.

Abstract: A method of biologically treating wastewater and, at the same time, incorporating processes that aim to increase the PHA accumulation potential of biomass used in the treatment of the wastewater. The method includes biologically treating the wastewater and enhancing PHA accumulation potential of the biomass by subjecting the biomass to a primary feast-famine process where the biomass is subjected to repeated cycles of feast and primary famine conditions. From time-to-time, the method entails deviating from the primary feast-famine process to a secondary famine process. The secondary famine process comprises subjecting the biomass to secondary famine conditions for a period of time that is substantially greater than the average time period of the primary famine conditions.

Abstract: A method of biologically treating wastewater and, at the same time, incorporating processes that aim to increase the PHA accumulation potential of biomass used in the treatment of the wastewater. The method includes biologically treating the wastewater and enhancing PHA accumulation potential of the biomass by subjecting the biomass to a primary feast-famine process where the biomass is subjected to repeated cycles of feast and primary famine conditions. From time-to-time, the method entails deviating from the primary feast-famine process to a secondary famine process. The secondary famine process comprises subjecting the biomass to secondary famine conditions for a period of time that is substantially greater than the average time period of the primary famine conditions.

Abstract: This disclosure relates to methods for producing a PHA-rich-biomass from open mixed cultures. Mixed liquor containing biomass is directed into a fed-batch reactor. The reactor includes at least one biomass stimulating zone and at least one biomass maintenance zone. A feed containing RBCOD, bioavailable N, and bioavailable P is directed into the fed-batch reactor. The respiration rate of at least a portion of the biomass is intermittently and repeatedly stimulated in the stimulating zone by directing the feed into the fed-batch reactor and exposing the biomass to a relatively high concentration of RBCOD. The biomass is then transferred to the maintenance zone, where the biomass is exposed to a relatively low concentration of RBCOD. Thereafter, the biomass is circulated back and forth between the stimulating zone and the maintenance zone.

Abstract: A method or process for producing polyhydroxyalkanoates (PHAs) in biomass. The process entails feeding an organic carbon containing substrate to biomass enriched in PHA accumulating bacteria. Particularly the process entails intermittently supplying the substrate to the biomass at least three separate times over a selected period. The object of the process is to produce PHA having a relatively high molecular weight, at least 400,000 g/mole. By controlling the frequency at which the substrate is supplied to the biomass and by feeding a sufficient amount of the substrate to the biomass, the method or process produces the PHA having the relatively high molecular weight.

Abstract: A method of biologically treating wastewater and removing contaminants from the wastewater is disclosed. In the course of treating the wastewater, biomass is produced. In addition to removing contaminants from the wastewater, the process or method of the present invention entails enhancing the PHA accumulation potential of the biomass. Disclosed are a number of processes that are employed in a biological wastewater treatment system for enhancing PHA accumulation potential. For example, enhanced PHA accumulation potential is realized by exposing the biomass to feast and famine conditions and, after exposing the biomass to famine conditions, stimulating the biomass into a period of feast by exposing the biomass to feast conditions for a selected period of time by applying an average peak stimulating RBCOD feeding rate of greater than 5 mg-COD\L\MIN in combination with an average peak specific RBCOD feeding rate greater than 0.5 mg-COD\g-VSS\MIN.

Abstract: A method or process for producing polyhydroxyalkanoates (PHAs) in biomass. The process entails feeding an organic carbon containing substrate to biomass enriched in PHA accumulating bacteria. Particularly the process entails intermittently supplying the substrate to the biomass at least three separate times over a selected period. The object of the process is to produce PHA having a relatively high molecular weight, at least 400,000 g/mole. By controlling the frequency at which the substrate is supplied to the biomass and by feeding a sufficient amount of the substrate to the biomass, the method or process produces the PHA having the relatively high molecular weight.

Abstract: A method or process is provided for treating wastewater and producing a polyhydroxyalkanote (PHA)-storing biomass. The method or process entails biologically treating wastewater and in one process a filamentous biomass is selected and caused to proliferate so as to dominate an activated sludge. The filamentous biomass is utilized to treat the wastewater and to remove contaminants therefrom. As a part of this process, there is provided an enhancement for PHA production potential in the said biomass. This entails enhancing the PHA production potential of the filamentous biomass by subjecting the biomass to alternating feast and famine conditions where under feast conditions more biodegradable organic substrate is available to the filamentous biomass than under famine conditions. In another process, wastewater is treated with an activated sludge.

Abstract: A process is provided for increasing the production of PHA in a mixed culture biomass. In a first stage of the process, organic material associated with a substrate is converted to volatile fatty acids. In the case of a wastewater treatment process, if the wastewater includes sufficient volatile fatty acids (VFAs) to support the process, then it is unnecessary to convert organic material to VFAs. In a second stage of the process, an anaerobic-aerobic selection process is utilized to select glycogen accumulating organisms that cause these organisms to proliferate and dominate the open mixed culture biomass. By providing relatively high organic loading in the form of VFAs in the anaerobic treatment phase of the selection process, glycogen accumulating organisms having a relatively high level of stored glycogen are produced. In a third stage, the PHA accumulation process is practiced where the glycogen rich organisms are fed VFAs under anaerobic or aerobic conditions or combinations thereof.

Abstract: A process is provided for increasing the production of PHA in a mixed culture biomass. In a first stage of the process, organic material associated with a substrate is converted to volatile fatty acids. In the case of a wastewater treatment process, if the wastewater includes sufficient volatile fatty acids (VFAs) to support the process, then it is unnecessary to convert organic material to VFAs. In a second stage of the process, an anaerobic-aerobic selection process is utilized to select glycogen accumulating organisms that cause these organisms to proliferate and dominate the open mixed culture biomass. By providing relatively high organic loading in the form of VFAs in the anaerobic treatment phase of the selection process, glycogen accumulating organisms having a relatively high level of stored glycogen are produced. In a third stage, the PHA accumulation process is practiced where the glycogen rich organisms are fed VFAs under anaerobic or aerobic conditions or combinations thereof.