Abstract: A high-strength hyaluronate fiber, suitable for use in a surgical suture, a lifting thread, a tissue-engineering scaffold, etc., and a manufacturing method thereof are proposed. The method includes (a) controlling a water content of a hyaluronate having a weight average molecular weight of 100-3,000 kDa to 50-95 wt % to prepare a hyaluronate paste, (b) melting the hyaluronate paste having a controlled water content at a temperature ranging from room temperature to 100° C., and then extruding the hyaluronate paste through a nozzle, and (c) drying the extruded spinning fluid to form a fiber. The hyaluronate fiber has the water content of 5-25 wt %, the tensile strength of 3 to 15 kg/mm2, and a smooth surface with small cracks, so that the hyaluronate fiber can be used for a surgical suture, a lifting thread, a filler for cosmetic surgery, a tissue-engineering scaffold, etc.