Abstract: The present disclosure relates to a method for determining a target spot path, which is applied to a determining system including a shooting device and a locating device that are separate from each other, and a calibration device connected to the shooting device, the method comprising: S1. obtaining a three-dimensional partial image for an affected part and a virtual path located in the three-dimensional partial image; S2. matching a simulated two-dimensional image obtained based on projection of the three-dimensional partial image with a two-dimensional projection image obtained based on the affected part; S3. determining a surgical guide path on the two-dimensional projection image that corresponds to the virtual path according to position information of the virtual path on the simulated two-dimensional image, when the simulated two-dimensional image and the two-dimensional projection image are matched; and/or S4.