Abstract: A method of altering or transferring the cytotype of a plant line. In particular, transferring the cytotype of a transformation-recalcitrant plant line, e.g., a transformation-recalcitrant maize line, from a transformation-recalcitrant cytotype to a transformable cytotype so that the line becomes transformable while preserving its nuclear genome. The newly-transformable line may be produced using methods including backcrossing and/or haploid induction.

Abstract: The present invention is drawn to compositions and methods for improving transformation frequency. The compositions, synthetic selectable marker genes, are used in transformation methods and result in increased transformation frequency.

Abstract: This invention provides a method for generating transgenic plants with a low copy number. Plant cells are transformed with polynucleotides containing transcriptional cassettes designed to trigger silencing of a gene which is essential for the plant cell to survive the transformation and regeneration process. The present invention enables the recovery of an increased number of transgenic plants which have only one copy of each desired transcriptional cassette.

Abstract: This invention provides a method for generating transgenic plants with a low copy number. Plant cells are transformed with polynucleotides containing transcriptional cassettes designed to trigger silencing of a gene which is essential for the plant cell to survive the transformation and regeneration process. The present invention enables the recovery of an increased number of transgenic plants which have only one copy of each desired transcriptional cassette.

Abstract: The present invention is drawn to compositions and methods for improving transformation frequency. The compositions, synthetic selectable marker genes, are used in transformation methods and result in increased transformation frequency.

Abstract: This invention provides a method for generating transgenic plants with a low copy number. Plant cells are transformed with polynucleotides containing transcriptional cassettes designed to trigger silencing of a gene which is essential for the plant cell to survive the transformation and regeneration process. The present invention enables the recovery of an increased number of transgenic plants which have only one copy of each desired transcriptional cassette.

Abstract: This invention provides a method for generating transgenic plants with a low copy number. Plant cells are transformed with polynucleotides containing transcriptional cassettes designed to trigger silencing of a gene which is essential for the plant cell to survive the transformation and regeneration process. The present invention enables the recovery of an increased number of transgenic plants which have only one copy of each desired transcriptional cassette.

Abstract: The present invention provides novel expression control elements and methods for expressing a heterologous nucleotide sequence of interest in plants. Isolated nucleic acids, expression cassettes and vectors comprising the expression control elements are provided. Also provided are transformed plants, plant tissues, plant cells, plant seed, and the like, comprising the isolated nucleic acids, expression cassettes and vectors of the invention. Further provided are methods of expressing a heterologous nucleotide sequence of interest in a plant, plant tissue, plant cell, and the like.

Abstract: The present invention provides novel expression control elements and methods for expressing a heterologous nucleotide sequence of interest in plants. Isolated nucleic acids, expression cassettes and vectors comprising the expression control elements are provided. Also provided are transformed plants, plant tissues, plant cells, plant seed, and the like, comprising the isolated nucleic acids, expression cassettes and vectors of the invention. Further provided are methods of expressing a heterologous nucleotide sequence of interest in a plant, plant tissue, plant cell, and the like.