Abstract: The present invention provides a detecting apparatus, including a storage chamber containing a treating fluid; wherein, the detecting apparatus is internally provided with a sharp-pointed portion; the storage chamber may make a movement relative to the sharp-pointed portion; the storage chamber will be pierced by the sharp-pointed portion during the moving process, such that the treating fluid in the storage chamber is released. The detecting apparatus further includes a collecting chamber; the released treating fluid may flow into the collecting chamber; the collecting chamber is disposed inside a first shell and used to contain a sample; an opening is disposed at an upper position of the first shell; the collecting chamber is internally provided with a testing element for detecting an analyte; the testing element is disposed on a carrier, and the carrier has a specific matching form with the collecting chamber.