Patents by Inventor Slavko Kralj

Slavko Kralj has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Engineered glucosyltransferases
    Patent number: 11952598
    Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
    Type: Grant
    Filed: June 4, 2021
    Date of Patent: April 9, 2024
    Assignee: NUTRITION & BIOSCIENCES USA 4, INC.
    Inventors: Yougen Li, Mark S. Payne, Jared B. Parker, Slavko Kralj, Veli Alkan, Richard R. Bott, Robert Dicosimo, Qiong Cheng, Ellen D. Semke, Susan Marie Hennessey
  • MODIFICATION OF STEVIOL GLYCOSIDES BY GLUCOSYLTRANSFERASE ENZYMES
    Publication number: 20240108021
    Abstract: Disclosed herein are methods of glucosylating a steviol glycoside. These methods can comprise providing a composition that comprises at least (i) water, (ii) sucrose, (iii) stevioside or rebaudioside A, and (iv) a glucosyltransferase enzyme that synthesizes alpha-1,6-glucan or a glucosyltransferase enzyme that synthesizes alpha-1,3-glucan, wherein at least one glucosylated form of the stevioside or rebaudioside A is produced in the composition. Such a composition can be a food product/precursor, for instance.
    Type: Application
    Filed: September 26, 2023
    Publication date: April 4, 2024
    Inventors: Karina Hansen Kjaer, Slavko Kralj
  • Compositions and methods comprising the use of and ?-glucanotransferase enzymes
    Patent number: 11884942
    Abstract: An isolated and/or purified ?-glucanotransferase from Exiguobacterium acetylicum, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the ?-glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the ?-glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.
    Type: Grant
    Filed: July 22, 2021
    Date of Patent: January 30, 2024
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventor: Slavko Kralj
  • Compositions and methods comprising the use of a inulosucrase (INUO)
    Patent number: 11859216
    Abstract: Bacillus agaradhaerens strain WDG185 expresses an inulosucrase that efficiently synthesizes a broad range of IOS with a GF range of GF3-GF30. The isolated and/or purified inulosucrase, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the inulosucrase, its variants, or its active fragments, host cells comprising the synthetic nucleic acids, and compositions comprising the inulosucrase are provided. Methods of using the compositions include the manufacture of inulooligosaccharides.
    Type: Grant
    Filed: September 17, 2020
    Date of Patent: January 2, 2024
    Assignee: Danisco US Inc.
    Inventors: Slavko Kralj, Marc Kolkman, Chris Leeflang, Johannes G. De Nobel, Arjen Hoekstra, Veli Alkan
  • Alpha-glucose-1-phosphate synthesis from sucrose and glucan synthesis using glucan phosphorylases
    Patent number: 11802275
    Abstract: Reactions are disclosed herein comprising water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and an alpha-1,4-glucan phosphorylase. Novel alpha-1,4-glucan phosphorylase enzymes are also disclosed. Additional disclosures herein regard sucrose phosphorylase enzymes and methods of use thereof to produce alpha-G1P.
    Type: Grant
    Filed: July 18, 2019
    Date of Patent: October 31, 2023
    Assignee: Danisco US Inc.
    Inventors: Slavko Kralj, Zheyong Yu, Zhenghong Zhang
  • Synthesis of glucan comprising alpha-1,3 glycosidic linkages with phosphorylase enzymes
    Patent number: 11473116
    Abstract: Reaction compositions are disclosed herein comprising at least water, beta-glucose -1-phosphate (beta-G1P), an acceptor molecule, and an alpha-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with alpha-1,3 glycosidic linkages. Further disclosed are alpha-1,3-glucan phosphorylase enzymes and methods of use thereof.
    Type: Grant
    Filed: October 19, 2020
    Date of Patent: October 18, 2022
    Assignee: NUTRITION & BIOSCIENCES USA 4, INC.
    Inventors: Zheyong Yu, Slavko Kralj, Zhenghong Zhang, Laurie A. Howe
  • SYNTHESIS OF GLUCAN COMPRISING BETA-1,3 GLYCOSIDIC LINKAGES WITH PHOSPHORYLASE ENZYMES
    Publication number: 20220195399
    Abstract: Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.
    Type: Application
    Filed: November 17, 2021
    Publication date: June 23, 2022
    Inventors: Zheyong Yu, Slavko Kralj, Natnael Behabtu, Zhenghong Zhang, Laurie A. Howe
  • COMPOSITIONS AND METHODS COMPRISING THE USE OF EXIGUOBACTERIUM ACETYLICUM AND BACILLUS COAGLUANS ALPHA-GLUCANOTRANSFERASE ENZYMES
    Publication number: 20220090030
    Abstract: An isolated and/or purified ?-glucanotransferase from Exiguobacterium acetylicum, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the ?-glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the ?-glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.
    Type: Application
    Filed: July 22, 2021
    Publication date: March 24, 2022
    Inventor: Slavko Kralj
  • COMPOSITIONS AND METHODS COMPRISING THE USE OF A BACILLUS AGARADHAERENS INULOSUCRASE (INUO)
    Publication number: 20220002685
    Abstract: Bacillus agaradhaerens strain WDG185 expresses an inulosucrase that efficiently synthesizes a broad range of IOS with a GF range of GF3-GF30. The isolated and/or purified inulosucrase, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the inulosucrase, its variants, or its active fragments, host cells comprising the synthetic nucleic acids, and compositions comprising the inulosucrase are provided. Methods of using the compositions include the manufacture of inulooligosaccharides.
    Type: Application
    Filed: September 17, 2020
    Publication date: January 6, 2022
    Applicant: DANISCO US INC.
    Inventors: Slavko KRALJ, Marc Kolkman, Chris Leeflang, Johannes G. De Nobel, Arjen Hoekstra, Veli Alkan
  • ENGINEERED GLUCOSYLTRANSFERASES
    Publication number: 20210395705
    Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
    Type: Application
    Filed: June 4, 2021
    Publication date: December 23, 2021
    Inventors: Yougen Li, MARK S. PAYNE, JARED B. PARKER, SLAVKO KRALJ, VELI ALKAN, RICHARD R. BOTT, ROBERT DICOSIMO, QIONG CHENG, ELLEN D. SEMKE, SUSAN MARIE HENNESSEY
  • Synthesis of glucan comprising beta-1,3 glycosidic linkages with beta-1,3-glucan phosphorylase enzymes
    Patent number: 11180740
    Abstract: Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.
    Type: Grant
    Filed: April 15, 2019
    Date of Patent: November 23, 2021
    Assignee: DANISCO US INC
    Inventors: Zheyong Yu, Slavko Kralj, Natnael Behabtu, Zhenghong Zhang, Laurie A Howe
  • Compositions and methods comprising the use of exiguobacterium acetylicum and bacillus coagluans alpha-glucanotransferase enzymes
    Patent number: 11072783
    Abstract: An isolated and/or purified ?-glucanotransferase from Exiguobacterium Acetylicum, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the ?-glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the ?-glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.
    Type: Grant
    Filed: October 6, 2016
    Date of Patent: July 27, 2021
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventor: Slavko Kralj
  • SYNTHESIS OF GLUCAN COMPRISING ALPHA-1,3 GLYCOSIDIC LINKAGES WITH PHOSPHORYLASE ENZYMES
    Publication number: 20210207184
    Abstract: Reaction compositions are disclosed herein comprising at least water, beta-glucose-1-phosphate (beta-G1P), an acceptor molecule, and an alpha-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with alpha-1,3 glycosidic linkages. Further disclosed are alpha-1,3-glucan phosphorylase enzymes and methods of use thereof.
    Type: Application
    Filed: October 19, 2020
    Publication date: July 8, 2021
    Inventors: ZHEYONG YU, SLAVKO KRALJ, ZHENGHONG ZHANG, LAURIE A. HOWE
  • Engineered glucosyltransferases
    Patent number: 11028373
    Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
    Type: Grant
    Filed: April 11, 2019
    Date of Patent: June 8, 2021
    Assignee: NUTRITION & BIOSCIENCES USA 4, INC.
    Inventors: Yougen Li, Mark S. Payne, Jared B. Parker, Slavko Kralj, Veli Alkan, Richard R. Bott, Robert Dicosimo, Qiong Cheng, Ellen D. Semke, Susan Marie Hennessey
  • ALPHA-GLUCOSE-1-PHOSPHATE SYNTHESIS FROM SUCROSE AND GLUCAN SYNTHESIS USING GLUCAN PHOSPHORYLASES
    Publication number: 20210163902
    Abstract: Reactions are disclosed herein comprising water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and an alpha-1,4-glucan phosphorylase. Novel alpha-1,4-glucan phosphorylase enzymes are also disclosed. Additional disclosures herein regard sucrose phosphorylase enzymes and methods of use thereof to produce alpha-G1P.
    Type: Application
    Filed: July 18, 2019
    Publication date: June 3, 2021
    Inventors: Slavko KRALJ, Zheyong YU, Zhenghong ZHANG
  • Synthesis of glucan comprising alpha-1,3 glycosidic linkages with phosphorylase enzymes
    Patent number: 10808269
    Abstract: Reaction compositions are disclosed herein comprising at least water, beta-glucose-1-phosphate (beta-G1P), an acceptor molecule, and an alpha-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with alpha-1,3 glycosidic linkages. Further disclosed are alpha-1,3-glucan phosphorylase enzymes and methods of use thereof.
    Type: Grant
    Filed: February 21, 2019
    Date of Patent: October 20, 2020
    Assignee: DANISCO US INC
    Inventors: Zheyong Yu, Slavko Kralj, Zhenghong Zhang, Laurie A. Howe
  • Engineered glucosyltransferases
    Patent number: 10774315
    Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes synthesize alpha-glucan products having increased molecular weight. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: September 15, 2020
    Assignee: DUPONT INDUSTRIAL BIOSCIENCES USA, LLC
    Inventors: Yougen Li, Ellen D. Semke, Mark S. Payne, Jared B. Parker, Susan Marie Hennessey, Slavko Kralj, Veli Alkan, Richard Bott
  • Variant enzymes
    Patent number: 10479983
    Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.
    Type: Grant
    Filed: July 24, 2014
    Date of Patent: November 19, 2019
    Assignee: DANISCO US INC
    Inventors: Nicholai Douglas, Maria Foukaraki, Ronaldus Wilhelmus Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Suzanne E. Lantz, Jonathan K. Lassila, Igor Nikolaev, Daniel Esteban Torres-Pazmino, Wilhelmus Van Der Kley, Johannes Franciscus Thomas Van Lieshout, Sander Van Stigt Thans
  • SYNTHESIS OF GLUCAN COMPRISING BETA-1,3 GLYCOSIDIC LINKAGES WITH PHOSPHORYLASE ENZYMES
    Publication number: 20190322990
    Abstract: Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.
    Type: Application
    Filed: April 15, 2019
    Publication date: October 24, 2019
    Inventors: ZHEYONG YU, SLAVKO KRALJ, NATNAEL BEHABTU, ZHENGHONG ZHANG, LAURIE A HOWE
  • ENGINEERED GLUCOSYLTRANSFERASES
    Publication number: 20190292528
    Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
    Type: Application
    Filed: April 11, 2019
    Publication date: September 26, 2019
    Inventors: Yougen Li, Mark S. Payne, Jared B. Parker, Slavko Kralj, Veli Alkan, Richard R. Bott, Robert Dicosimo, Qiong Cheng, Ellen D. Semke, Susan Marie Hennessey