Patents by Inventor Snezana Drmanac

Snezana Drmanac has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210254150
    Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.
    Type: Application
    Filed: October 29, 2020
    Publication date: August 19, 2021
    Inventors: Radoje Drmanac, Snezana Drmanac, Handong Li, Xun Xu, Matthew J. Callow, Leon Eckhardt, Naibo Yang
  • Publication number: 20210206794
    Abstract: Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.
    Type: Application
    Filed: March 22, 2021
    Publication date: July 8, 2021
    Inventors: Handong Li, Snezana Drmanac, Radoje Drmanac, Xun Xu, Lingling Peng, Scott Gablenz
  • Patent number: 10988501
    Abstract: Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.
    Type: Grant
    Filed: April 21, 2017
    Date of Patent: April 27, 2021
    Inventors: Handong Li, Snezana Drmanac, Radoje Drmanac, Xun Xu, Lingling Peng, Scott Gablenz
  • Patent number: 10961519
    Abstract: Provided are a phi29 DNA polymerase mutant with increased thermo stability, a method for preparing the mutant, the use of the mutant, and a method for increasing the stability of the phi29 DNA polymerase.
    Type: Grant
    Filed: October 18, 2019
    Date of Patent: March 30, 2021
    Assignee: MGI TECH CO., LTD.
    Inventors: Huanhuan Liu, Yue Zheng, Yujun Zhou, Xi Zhang, Zhougang Zhang, Yuliang Dong, Wenwei Zhang, Chongjun Xu, Snezana Drmanac
  • Patent number: 10883092
    Abstract: Provided are a phi29 DNA polymerase and an encoding gene and an application thereof. The phi29 DNA polymerase is C1) or C2): C1) is a protein with DNA polymerase activity obtained by substituting at least one of the 58th, 61st, 94th, 96th, 119th, and 155th amino acid residues in the amino acid sequence of a wild type phi29 DNA polymerase as shown in SEQ ID NO: 2 in the sequence listing; and C2) is a fusion protein obtained by linking a label to the N-terminus and/or C-terminus of the protein represented by C1). A 3?-5?exonuclease of the phi29 DNA polymerase has activity lower than that of the wild type phi29 DNA polymerase, and can efficiently and continuously synthesize DNA during amplification and sequencing.
    Type: Grant
    Filed: April 18, 2017
    Date of Patent: January 5, 2021
    Assignee: MGI TECH CO., LTD.
    Inventors: Yue Zheng, Zhougang Zhang, Yuliang Dong, Wenwei Zhang, Chongjun Xu, Snezana Drmanac
  • Patent number: 10883091
    Abstract: Disclosed in the present disclosure is a recombinant DNA polymerase. The recombinant DNA polymerase is any one selected from: A) a protein, having amino acid modifications at positions 408, 409 and 485, and at least one of amino acid modification(s) at positions 53, 59, 199, 243, 526, 558, 613, 641, 671, 673, 674, 692 and 709 compared to the amino acid sequence of a wild-type KOD DNA polymerase; B) a protein derived from the protein in A), formed by deleting amino acids 1 to 29 from a C-terminus of the protein in A) and keeping the remaining amino acids unchanged; and C) a protein derived from the protein in A) or B), formed by connecting a tag to the N-terminus or C-terminus of the amino acid sequence of the protein in A) or B), wherein the protein in A), B) and C) has DNA polymerase activity.
    Type: Grant
    Filed: July 8, 2019
    Date of Patent: January 5, 2021
    Assignee: MGI TECH CO., LTD.
    Inventors: Lin Wang, Fen Liu, Yuliang Dong, Wenwei Zhang, Chongjun Xu, Snezana Drmanac
  • Publication number: 20200392574
    Abstract: Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer.
    Type: Application
    Filed: August 14, 2020
    Publication date: December 17, 2020
    Inventors: Radoje Drmanac, Matthew J. Callow, Snezana Drmanac, Brian K. Hauser, George Yeung
  • Patent number: 10851410
    Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.
    Type: Grant
    Filed: January 4, 2018
    Date of Patent: December 1, 2020
    Assignees: MGI Tech Co., Ltd., BGI Shenzhen
    Inventors: Radoje Drmanac, Snezana Drmanac, Handong Li, Xun Xu, Matthew J. Callow, Leon Eckhardt, Naibo Yang
  • Publication number: 20200115686
    Abstract: Provided are a phi29 DNA polymerase mutant with increased thermo stability, a method for preparing the mutant, the use of the mutant, and a method for increasing the stability of the phi29 DNA polymerase.
    Type: Application
    Filed: October 18, 2019
    Publication date: April 16, 2020
    Inventors: Huanhuan Liu, Yue Zheng, Yujun Zhou, Xi Zhang, Zhougang Zhang, Yuliang Dong, Wenwei Zhang, Chongjun Xu, Snezana Drmanac
  • Publication number: 20200115748
    Abstract: Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer.
    Type: Application
    Filed: May 29, 2019
    Publication date: April 16, 2020
    Inventors: Radoje Drmanac, Matthew J. Callow, Snezana Drmanac, Brian K. Hauser, George Yeung
  • Publication number: 20200048620
    Abstract: Provided are a phi29 DNA polymerase and an encoding gene and an application thereof. The phi29 DNA polymerase is C1) or C2): C1) is a protein with DNA polymerase activity obtained by substituting at least one of the 58th, 61st, 94th, 96th, 119th, and 155th amino acid residues in the amino acid sequence of a wild type phi29 DNA polymerase as shown in SEQ ID NO: 2 in the sequence listing; and C2) is a fusion protein obtained by linking a label to the N-terminus and/or C-terminus of the protein represented by C1). A 3?-5?exonuclease of the phi29 DNA polymerase has activity lower than that of the wild type phi29 DNA polymerase, and can efficiently and continuously synthesize DNA during amplification and sequencing.
    Type: Application
    Filed: April 18, 2017
    Publication date: February 13, 2020
    Inventors: Yue ZHENG, Zhougang ZHANG, Yuliang DONG, Wenwei ZHANG, Chongjun XU, Snezana DRMANAC
  • Publication number: 20190271037
    Abstract: This application discloses methods of producing a DNA strand for sequencing, as well as genetic constructs, libraries, and arrays using DNA strands produced according to these methods. The application also discloses methods of sequencing using the DNA strands, genetic constructs, libraries, and arrays produced. In certain aspects, DNA being sequenced includes a target sequence and at least one adaptor sequence.
    Type: Application
    Filed: March 8, 2019
    Publication date: September 5, 2019
    Inventors: Rongqin Ke, Snezana Drmanac, Radoje Drmanac, Guangyang Cai, Matthew Callow
  • Patent number: 10351909
    Abstract: The invention relates to an automated method for high-throughput DNA sequencing from high density DNA arrays by (a) initiating a first sequencing reaction on a first high density DNA array; and imaging said first high density DNA array using a detector, and (b) initiating a first sequencing reaction on a second high density DNA array; and imaging said second high density DNA array using the detector, wherein the first sequencing reaction in (a) is initiated before the first sequencing reaction in (b) is initiated such that the sequencing reactions in (a) and (b) are staggered. By using asynchronous sequencing reactions and imaging two separate arrays using one detector, imaging can be carried out on one array while sequencing reactions are carried out on one the other, substrate, the other substrate is imaged, reducing the idle time of the imaging system.
    Type: Grant
    Filed: February 25, 2017
    Date of Patent: July 16, 2019
    Assignee: Complete Genomics, Inc.
    Inventors: Radoje Drmanac, Matthew J. Callow, Snezana Drmanac, Brian K. Hauser, George Yeung
  • Publication number: 20190144482
    Abstract: Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.
    Type: Application
    Filed: April 21, 2017
    Publication date: May 16, 2019
    Inventors: Handong Li, Snezana Drmanac, Radoje Drmanac, Xun Xu, Lingling Peng, Scott Gablenz
  • Patent number: 10227647
    Abstract: This application discloses methods of producing a DNA strand for sequencing, as well as genetic constructs, libraries, and arrays using DNA strands produced according to these methods. The application also discloses methods of sequencing using the DNA strands, genetic constructs, libraries, and arrays produced. In certain aspects, DNA being sequenced includes a target sequence and at least one adaptor sequence.
    Type: Grant
    Filed: February 10, 2016
    Date of Patent: March 12, 2019
    Assignee: Complete Genomics, Inc.
    Inventors: Rongqin Ke, Snezana Drmanac, Radoje Drmanac, Guangyang Cai, Matthew Callow
  • Patent number: 10190162
    Abstract: Novel fluorescent nucleotide analogs are provided herein. Also provided herein are methods of using the nucleotide analogs in sequencing-by-synthesis and signal confinement methods.
    Type: Grant
    Filed: October 23, 2015
    Date of Patent: January 29, 2019
    Assignee: Complete Genomics, Inc.
    Inventors: Snezana Drmanac, Handong Li, Radoje Drmanac, Eric Harness, Chongjun Xu
  • Publication number: 20190010542
    Abstract: The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.
    Type: Application
    Filed: March 29, 2018
    Publication date: January 10, 2019
    Applicant: Complete Genomics Inc.
    Inventors: Radoje Drmanac, Matthew J. Callow, Snezana Drmanac
  • Publication number: 20180350259
    Abstract: Exemplary embodiments of the present invention beneficially provide systems, computer-implemented methods, and computer-readable program products to facilitate a plurality of user-driven virtual sponsoring sessions for a plurality of users through one or more user-designed virtual sponsors where the topic of the virtual sponsoring session, the type of virtual sponsoring session, and the form of each virtual sponsoring statement is controlled by each of the plurality of users. The virtual sponsoring sessions can be for any subject matter domain through user-designed virtual sponsors that have no domain-specific knowledge, such that users are provided with real-time, on-demand access to virtual sponsoring services via a user-designed virtual sponsor designed and constructed responsive to the personality, preferences, and specifications of each individual user.
    Type: Application
    Filed: March 7, 2018
    Publication date: December 6, 2018
    Inventors: Radoje T. Drmanac, Snezana Drmanac
  • Publication number: 20180223358
    Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.
    Type: Application
    Filed: January 4, 2018
    Publication date: August 9, 2018
    Inventors: Radoje Drmanac, Snezana Drmanac, Handong Li, Xun Xu, Matthew J. Callow, Leon Eckhardt, Naibo Yang, Quan Ding
  • Publication number: 20180180567
    Abstract: Provided is a microwell electrode, comprising one or more first electrodes (301); one or more second electrodes (303) each arranged opposite to one first electrode (301), wherein a channel (601) is provided between each first electrode and the second electrode opposite thereto, and the channel (601) has at least one end in communication with a chamber; and one or more guiding electrodes (501) located in the chamber (401). The microwell electrode electrode can sensitively detect a signal and improve the read length of a sequencer greatly. The invention further relates to a method for manufacturing the micro-porous electrode, a microwell electrode array, a sensor chip, a sequencing system, and a method for analysis of a chemical substance and a nucleic acid molecule based on the microwell electrode.
    Type: Application
    Filed: June 23, 2016
    Publication date: June 28, 2018
    Inventors: Handong LI, Jianxun LIN, Quanxin YUN, Shaohua XIANG, Radoje DRMANAC, Snezana DRMANAC, Yongwei ZHANG