Abstract: A method for visualizing a macromolecule fragment pattern resulting from gel electrophoresis has steps for preparing a polyacrylamide gel in an electrophoresis apparatus, treating the polyacrylamide gel with Meldrum's acid Activated Furan (MAF) dissolved in a suitable solvent, loading a protein sample to a prepared position in the gel, and resolving the protein sample by applying an electrical potential across the gel for a period of time, separating molecular fragments of the protein into the fragment pattern in the gel. The method is characterized in that the MAF stains the molecular fragments in a manner to be discernible by human vision and by conventional analysis techniques.

Abstract: The invention discloses a detergent-compatible protein assay method, composition and kit based on bio-conjugation reaction between protein and Meldrum's acid activated furfural. The method includes adding MAF in dimethyl sulfoxide (DMSO) to a protein sample solution. The amine functionalities present on the amino acid residues reacts with the MAF instantaneously at room temperature to yield deep purple colored solutions of the corresponding conjugated proteins. The reagent composition added to protein may be in the range of 90-450 mM. The intensities of purple colored solutions were proportional to the protein concentration captured by spectrophotometric measurements. The assay is sensitive in the range of 0.125-15 mg/mL, is compatible with commonly used detergents and reducing agents in protein solutions and may be employed for estimation of protein samples in the presence of detergents and reducing agents.

Abstract: The invention discloses a detergent-compatible protein assay method, composition and kit based on bio-conjugation reaction between protein and Meldrum's acid activated furfural. The method includes adding MAF in dimethyl sulfoxide (DMSO) to a protein sample solution. The amine functionalities present on the amino acid residues reacts with the MAF instantaneously at room temperature to yield deep purple colored solutions of the corresponding conjugated proteins. The reagent composition added to protein may be in the range of 90-450 mM. The intensities of purple colored solutions were proportional to the protein concentration captured by spectrophotometric measurements. The assay is sensitive in the range of 0.125-15 mg/mL, is compatible with commonly used detergents and reducing agents in protein solutions and may be employed for estimation of protein samples in the presence of detergents and reducing agents.