Abstract: A hydrogel particle, comprising a matrix comprising a polymerized monomer, said matrix comprising a plurality of micropores and a plurality of macropores, and one or more immunostimulatory biomolecules selected from the group consisting of an anti-CD3 antibody or antigen-binding fragment thereof, an anti-CD28 antibody or antigen-binding fragment thereof, and combinations thereof.

Abstract: A hydrogel particle, comprising a matrix comprising a polymerized monomer, said matrix comprising a plurality of micropores and a plurality of macropores, and one or more immunostimulatory biomolecules selected from the group consisting of an anti-CD3 antibody or antigen-binding fragment thereof, an anti-CD28 antibody or antigen-binding fragment thereof, and combinations thereof.

Abstract: A hydrogel particle, comprising a matrix comprising a polymerized monomer, said matrix comprising a plurality of micropores and a plurality of macropores, and one or more immunostimulatory biomolecules selected from the group consisting of an anti-CD3 antibody or antigen-binding fragment thereof, an anti-CD28 antibody or antigen-binding fragment thereof, and combinations thereof.

Abstract: The present disclosure relates to compositions and methods of generating circular-permuted nucleic acids for direct use in homology directed genome editing. The method also allows the joining of a large number of DNA fragments, in a deterministic fashion. It can be used to rapidly generate nucleic acid libraries that can be directly used in a variety of applications without further cloning steps that include, for example, genome editing and pathway assembly. Kits for performing the method are also disclosed.

Abstract: The invention provides methods of engineering latex-producing plants, e.g., guayule plants, to increase latex production. The invention additionally provides plants engineered in accordance with the invention and methods of using the plants to produce latex.

Abstract: The invention provides methods of engineering guayule plants to increase latex production. Specifically, the invention provides a method of engineering guayule plants with gene constructs that allow expression of master regulators of stress responses under non-stress conditions, the method comprising: introducing an expression cassette into the guayule plant, wherein the expression cassette comprises a polynucleotide encoding a dehydration response element binding protein/C-repeat binding factors (DREB/CBF) or DEAR [DREB and C-terminal EAR (ethylene response factor associated amphiphilic repression) motif] transcription factor operably linked to a promoter, such as a stem-specific promoter or an alcohol-inducible promoter. The invention additionally provides plants engineered in accordance with the invention and methods of using the plants to produce latex.