Abstract: Provided is a transgenic plant production method for producing a genetically modified plant. The method is capable of efficient short term gene transfer, of introducing a target gene while retaining the useful traits of a plant undergoing gene transfer, and of efficient redifferentiation. The transgenic plant production method for producing a genetically modified plant includes a culture step of culturing a tissue fragment derived from a target plant to obtain a cultured tissue fragment, a transformation step of transforming, with a gene construct, the cultured tissue fragment obtained in the culture step, and a regeneration step of regenerating a plant from the tissue fragment transformed in the transformation step.

Abstract: The invention provides: a callus induction method that efficiently induces callus from a tissue fragment from an isoprenoid-producing plant; a callus culture method that efficiently grows callus; a somatic embryo induction method that efficiently forms a somatic embryo; a plant regeneration method that can stably regenerate callus into plants; a plant propagation method that can stably propagate a plant without being affected by e.g. weather and seasons; and a method of inducing rooting in a mature embryo. The present invention relates, inter alia, to a method of plant regeneration that can stably regenerate callus into plants and a method of plant propagation that can stably propagate a plant.

Abstract: Provided are a method of regenerating a rubber tree that can stably regenerate the rubber tree from a tissue including a node, axillary bud or apical bud derived from a mature rubber tree, a young rubber tree, or a plantlet of a rubber tree; and a method of propagating a rubber tree that can stably acquire large numbers of clone seedlings of the rubber tree. Included is a method of regenerating a rubber tree including an induction step of culturing a tissue including a node, axillary bud or apical bud of a rubber tree in an induction medium containing a plant growth hormone and a carbon source to form a shoot.