Abstract: Aspects of the disclosure relate to multi-factor authentication based on biological signals. A computing platform may detect an indication to authenticate a user of the user device. Subsequently, the computing platform may trigger, based on the detecting, the at least one physical sensor to capture one or more biological signals emitted by the user. Then, the computing platform may transform each of the one or more captured biological signals to binary valued data. Then, the computing platform may generate, based on the binary valued data, a security credential associated with the user. Subsequently, the computing platform may retrieve, from a database, a stored security credential. Then, the computing platform may compare the generated security credential with the stored security credential. Subsequently, the computing platform may, based on a determination that the generated security credential is within a threshold of the stored security credential, authenticate the user of the user device.