Abstract: The invention is generally related to MCs containing sorghum centromere sequences. In addition, the invention provides for methods of generating plants transformed with these MCs. MCs with novel compositions and structures are used to transform plants cells which are in turn used to generate the plant. Methods for generating the plant include methods for delivering the MC into plant cell to transform the cell, methods for selecting the transformed cell, and methods for isolating plants transformed with the MC.

Abstract: Systems, methods, and apparatus, including computer programs encoded on computer storage media, for facilitating secure communication. A system for facilitating secure communication includes an enterprise network, one or more operational technology networks, and a management server. Each of the operational technology networks can include one or more controller devices operable to control one or more operational devices, and can include a respective site security server and a respective security relay server. The security relay server can be operable to facilitate secure communication between controller devices of the operational technology network and its corresponding site security server. The management server can be a node on the enterprise network and can be operable to communicate with each site security server.

Abstract: Systems, methods, and apparatus, including computer programs encoded on computer storage media, for facilitating communication in an industrial control network. A system includes an industrial control network, one or more controller devices, one or more emulators, and an encryption relay processor. Each controller device can be operable to control one or more operational devices connected to the industrial control network. Each emulator can be configured to communicate with a respective controller device, and each emulator can be configured to reference a respective profile that includes information about security capabilities of the respective controller device. The encryption relay processor can be operable to facilitate communication to and from each emulator over the industrial control network.

Abstract: The invention is generally related to Sugarcane mini-chromosomes and recombinant chromosomes containing Sugarcane centromere sequences. In addition, the invention provides for methods of generating Sugarcane plants transformed with these Sugarcane mini-chromosomes. Sugarcane mini-chromosomes with novel compositions and structures are used to transform Sugarcane cells which are in turn used to generate Sugarcane plants. Methods for generating Sugarcane plants include methods for delivering the Sugarcane mini-chromosomes into Sugarcane cell to transform the cell, methods for selecting the transformed cell, and methods for isolating Sugarcane plants transformed with the Sugarcane mini-chromosome or recombinant chromosome.

Abstract: The present invention relates to methods allowing the targeted insertion of transgenes into a plant genome at desired loci by using homologous recombination combined with rare-cutting endonucleases without the need of inserting an exogenous selectable marker.

Abstract: The invention is generally related to MCs containing sorghum centromere sequences. In addition, the invention provides for methods of generating plants transformed with these MCs. MCs with novel compositions and structures are used to transform plants cells which are in turn used to generate the plant. Methods for generating the plant include methods for delivering the MC into plant cell to transform the cell, methods for selecting the transformed cell, and methods for isolating plants transformed with the MC.

Abstract: A transformer skeleton and a winding method using the same are provided. The transformer skeleton includes at least one limiting hook. The limiting hook and a pin of the transformer skeleton are located on a same side of the transformer skeleton. A wire, which is led out from a winding groove of the transformer skeleton, electrically contacts the pin and passes through the limiting hook. The limiting hook is used to prevent the wire from moving upward. The advantage of the transformer skeleton is that the transformer skeleton disposes at least one limiting hook which is used to prevent the wire from moving upward and only a single layer of the wire contacts the pin in order to reduce the height of a tin cone. The transformer skeleton can decrease the height of the tin cone by 70%˜80%, thereby efficiently decreasing the height and width of the transformer.

Abstract: The invention is generally related to Sugarcane mini-chromosomes and recombinant chromosomes containing Sugarcane centromere sequences. In addition, the invention provides for methods of generating Sugarcane plants transformed with these Sugarcane mini-chromosomes. Sugarcane mini-chromosomes with novel compositions and structures are used to transform Sugarcane cells which are in turn used to generate Sugarcane plants. Methods for generating Sugarcane plants include methods for delivering the Sugarcane mini-chromosomes into Sugarcane cell to transform the cell, methods for selecting the transformed cell, and methods for isolating Sugarcane plants transformed with the Sugarcane mini-chromosome or recombinant chromosome.

Abstract: The invention is generally related to sugar cane mini-chromosomes containing sugar cane centromere sequences. In addition, the invention provides for methods of generating sugar cane plants transformed with these sugar cane mini-chromosomes. Sugar cane mini-chromosomes with novel compositions and structures are used to transform sugar cane cells which are in turn used to generate sugar cane plants. Methods for generating sugar cane plants include methods for delivering the sugar cane mini-chromosomes into sugar cane cell to transform the cell, methods for selecting the transformed cell, and methods for isolating sugar cane plants transformed with the sugarcane mini-chromosome.

Abstract: The invention provides a film deposition apparatus, which includes a first cover and a second cover, wherein the first cover and the second cover are disposed opposite to each other, and the first cover has at least two holes, and a spacer disposed between the first cover and the second cover, wherein the first cover, the spacer and the second cover form a film deposition space.

Abstract: The invention is generally related to compositions and methods related to novel centromere sequences identified in cotton, and resulting recombinant DNA constructs, such as minichromosomes, made using such sequences. Minichromosomes with novel compositions and structures can be used, for example, to transform plant cells that are in turn used to generate minichromosome-harboring plants. The invention is directed to products of such plants, including oil and textiles. The invention is also directed to novel methods for identifying centromere sequences.

Abstract: The invention is generally related to MCs containing sorghum centromere sequences. In addition, the invention provides for methods of generating plants transformed with these MCs. MCs with novel compositions and structures are used to transform plants cells which are in turn used to generate the plant. Methods for generating the plant include methods for delivering the MC into plant cell to transform the cell, methods for selecting the transformed cell, and methods for isolating plants transformed with the MC.

Abstract: The invention provides a film deposition apparatus, which includes a first cover and a second cover, wherein the first cover and the second cover are disposed opposite to each other, and the first cover has at least two holes, and a spacer disposed between the first cover and the second cover, wherein the first cover, the spacer and the second cover form a film deposition space.

Abstract: The invention is generally related to Sugarcane mini-chromosomes and recombinant chromosomes containing Sugarcane centromere sequences. In addition, the invention provides for methods of generating Sugarcane plants transformed with these Sugarcane mini-chromosomes. Sugarcane mini-chromosomes with novel compositions and structures are used to transform Sugarcane cells which are in turn used to generate Sugarcane plants. Methods for generating Sugarcane plants include methods for delivering the Sugarcane mini-chromosomes into Sugarcane cell to transform the cell, methods for selecting the transformed cell, and methods for isolating Sugarcane plants transformed with the Sugarcane mini-chromosome or recombinant chromosome.

Abstract: The invention provides efficient methods for the isolation of centromeres from potentially any organism. Using the methods of the invention, methylated centromere DNA may be isolated from potentially any centromere in an organism. The technique is amenable to mass screenings employing use of arrays comprising libraries of DNA from a target species.

Abstract: The invention provides efficient methods for the isolation of centromeres from potentially any organism. The methods represents an advance over the prior art in that costly and labor intensive mapping programs are not required. Using the technique, methylated centromere DNA may be isolated from potentially any centromere in an organism. The technique is amenable to mass screenings employing use of arrays comprising libraries of DNA from a target species.

Abstract: The invention provides efficient methods for the isolation of centromeres from potentially any organism. The methods may comprise the steps of: a) preparing a first sample of genomic DNA from a selected species; b) obtaining a plurality of methylated nucleic acid segments from the genomic DNA; and c) screening the methylated nucleic acid segments to identify a centromere nucleic acid sequence.

Abstract: The invention provides efficient methods for the isolation of centromeres from potentially any organism. The methods represents an advance over the prior art in that costly and labor intensive mapping programs are not required. Using the technique, methylated centromere DNA may be isolated from potentially any centromere in an organism. The technique is amenable to mass screenings employing use of arrays comprising libraries of DNA from a target species.