Abstract: A method for making a silk protein film includes providing an aqueous solution of a silk protein, and annealing a mixture including the aqueous solution of the silk protein and a water-soluble polyhydroxy compound that is present in an amount ranging from 20 wt % to 60 wt % based on a total amount of the silk protein and the water-soluble polyhydroxy compound at an annealing temperature that is higher than 50° C. and lower than 180° C. and under a relative humidity of not higher than 30%, so as to form the silk protein film.

Abstract: A method for making a silk protein film includes providing an aqueous solution of a silk protein, and annealing a mixture including the aqueous solution of the silk protein and a water-soluble polyhydroxy compound that is present in an amount ranging from 20 wt % to 60 wt % based on a total amount of the silk protein and the water-soluble polyhydroxy compound at an annealing temperature that is higher than 50° C. and lower than 180° C. and under a relative humidity of not higher than 30%, so as to form the silk protein film.

Abstract: The present invention relates to Maruca vitrata cell lines established from pupal tissues of M. vitrata, including NTU-MV, two subclones of NTU-MV: NTU-MV-1 and NTU-MV-2. The NTU-MV cell line was confirmed to be derived from M. vitrata, and NTU-MV-1, and NTU-MV-2 were confirmed to be derived from NTU-MV by karyotype analysis, Internal transcribed spacer (ITS) analysis and isozyme analysis. The genotypes and characteristics of the abovementioned 3 cell lines are totally different from other insect cell lines so that they are newly established cell lines. In addition, these three MV cell lines are susceptible to Maruca vitrata multiple nucleopolyhedrovirus (MaviMNPV). Therefore the 3 cell lines according to invention can be applied in multiplication of MaviMNPV in vitro to produce biopesticides in pest control. In addition, the cell lines can also be used as hosts for the expression vectors of baculovirus to produce recombinant proteins.

Abstract: The present invention relates to Maruca vitrata cell lines established from pupal tissues of M. vitrata, including NTU-MV, two subclones of NTU-MV: NTU-MV-1 and NTU-MV-2. The NTU-MV cell line was confirmed to be derived from M. vitrata, and NTU-MV-1, and NTU-MV-2 were confirmed to be derived from NTU-MV by karyotype analysis, Internal transcribed spacer (ITS) analysis and isozyme analysis. The genotypes and characteristics of the abovementioned 3 cell lines are totally different from other insect cell lines so that they are newly established cell lines. In addition, these three MV cell lines are susceptible to Maruca vitrata multiple nucleopolyhedrovirus (MaviMNPV). Therefore the 3 cell lines according to invention can be applied in multiplication of MaviMNPV in vitro to produce biopesticides in pest control. In addition, the cell lines can also be used as hosts for the expression vectors of said baculovirus to produce recombinant proteins.