Abstract: The present invention relates to a signal peptide for reducing the end heterogeneity of a heterologous polypeptide. Specifically, the present invention relates to a signal peptide for protein expression. The present invention further relates to a recombinant polypeptide, a preparation method therefor, and a composition containing a recombinant polypeptide.

Abstract: Use of genomic NW_006882077.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 691045 in a CHO cell gene NW_006882077.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 690980-691090 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.

Abstract: Use of genomic NW_006880285.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 1235357 in a CHO cell gene NW_006880285.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 1235284-1235429 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.

Abstract: The invention provides use of NW_006883358.1 in a CHO cell genome for stable expression of a protein. A site for stable expression of a protein in the CHO cell genome is located at the 6874389th base of CHO cell gene NW_006883358.1; 5?NNNNNNNNNNNNNNNNNNNNNGG3? within a range of 6874325-6874443 near the site, which can be identified by CRISPR/Cas9 technology, is a target sequence. In the present application, different protein genes are introduced to a fixed position in the CHO cell genome for stable expression.

Abstract: Use of genomic NW_006882077.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 691045 in a CHO cell gene NW_006882077.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 690980-691090 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.

Abstract: Use of genomic NW_006880285.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 1235357 in a CHO cell gene NW_006880285.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 1235284-1235429 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.

Abstract: Dispensing packages are disclosed that are capable of dispensing soft-solid type antiperspirant compositions employing actives having a metal to chloride ratio of less than or equal to 1.3, and that are capable of exhibiting minimal weeping in between uses.

Abstract: A method for making an emulsified antiperspirant product is provided. The product has a continuous phase with a water-immiscible liquid and a disperse aqueous phase with an antiperspirant active. The method steps include providing a water-immiscible liquid; providing a volatile aqueous solution with an antiperspirant active; combining the water-immiscible liquid and the aqueous solution to form an emulsion with a continuous phase including the water-immiscible liquid and a disperse phase including the aqueous solution; providing a structurant separate from the emulsion and adding it to the emulsion to form an emulsified antiperspirant composition. The emulsified antiperspirant composition is heated to a temperature between 60° C. and 110° C. from 15 minutes to less than 2 hours. The emulsified antiperspirant composition is transferred into one or more dispensing containers. The batch weight of the emulsified antiperspirant composition may be greater than 20 kg.

Abstract: A personal care composition exhibiting a tan delta of about 0.30 or more at about 10° C. includes a primary surfactant, secondary surfactant, and a polyol. Methods are also provided to improve the stability of a personal care composition.

Abstract: Methods for making antiperspirant products that are in the form of emulsions comprising a continuous water-immiscible phase and a disperse aqueous phase.

Abstract: Methods for making antiperspirant products in the form of emulsions are provided, wherein the emulsion comprises a continuous water-immiscible phase and a disperse aqueous phase comprising an antiperspirant active. The method comprises the steps of providing a water-immiscible liquid, providing an aqueous solution comprising an antiperspirant active, preparing an emulsion comprising a continuous phase including the water-immiscible liquid and a disperse phase including the aqueous solution, heating the emulsion to a temperature between about 60° C. and about 110° C., providing a structurant that is at least partially dissolved, adding the heated emulsion to the at least partially dissolved structurant to form an emulsified antiperspirant composition, and transferring the emulsified antiperspirant into a dispensing container within about 2 hours of initiating the heating step.

Abstract: A personal care composition exhibiting a tan delta of about 0.30 or more at about 10° C. includes a primary surfactant, secondary surfactant, and a polyol. Methods are also provided to improve the stability of a personal care composition.

Abstract: Dispensing packages are disclosed that are capable of dispensing soft-solid type antiperspirant compositions employing actives having a metal to chloride ratio of less than or equal to 1.3, and that are capable of exhibiting minimal weeping in between uses.

Abstract: Methods for making antiperspirant products that are in the form of emulsions comprising a continuous water-immiscible phase and a disperse aqueous phase.

Abstract: Solid emulsion antiperspirant products are described. The antiperspirant products contain a continuous phase including a water-immiscible liquid and a structurant and a disperse phase including an antiperspirant active dissolved in a polar solvent. The disperse phase has a conductivity of less than or equal to about 75 mS/cm at 25° C. And the antiperspirant active has a combined peak 4 and peak 5 area percentage of at least about 25% relative to the area sum of peaks 1 to 5. Methods for making solid emulsion antiperspirant products are also described.

Abstract: Deodorant compositions comprising an emulsion including a continuous polar solvent phase and a discontinuous oil phase are provided. The oil phase comprises an oil ingredient and a fragrance material. Methods for making such deodorant compositions are also provided.