Abstract: Provided herein are methods of detecting pathogen antibodies, such as a severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), in samples. Related devices, kits, reaction mixtures, and systems are also provided.

Abstract: A method is provided to transform progenitor cells, fetal cells, stem cells or tumor cells, e.g., in a microfluidic device, with nucleic acid or protein.

Abstract: A vortex how based method for electroporating molecules, e.g., sequentially, into cells is provided.

Abstract: A device includes a microfluidic trap disposed along a microfluidic channel, the trap and channel having dimensions to create a fluid vortex within the trap to trap a particle of interest and an electrode having interdigitated electrically isolated fingers positioned in the trap to create an electric field across the trap such that the electric field causes electroporation of a molecule into the particle of interest. A further device includes an array of channels, traps and interdigitated electrically isolated fingers.

Abstract: A method is provided to transform progenitor cells, fetal cells, stem cells or tumor cells, e.g., in a micro-fluidic device, with nucleic acid or protein.

Abstract: A device includes a microfluidic trap disposed along a microfluidic channel, the trap and channel having dimensions to create a fluid vortex within the trap to trap a particle of interest and an electrode having interdigitated electrically isolated fingers positioned in the trap to create an electric field across the trap such that the electric field causes electroporation of a molecule into the particle of interest. A further device includes an array of channels, traps and interdigitated electrically isolated fingers.

Abstract: A vortex flow based method for electroporating molecules, e g., sequentially, into cells is provided. The invention provides a method to determine the effect of two or more molecules of interest on cells. The method comprises maintaining a vortex flow in multiple traps having cells of interest; providing a first molecule of interest to the traps and an electric field across the traps for a defined first period of time; providing a second molecule of interest to the traps and an electric field across the traps for a defined second period of time; and determining the combined effect of the first and second molecules on the cells, e.g., whether the combined effect of the first and second molecules on the cells is synergistic, neutral or antagonistic.

Abstract: A system and method include delivering cells of interest to multiple traps via a channel connecting the traps, maintaining a vortex flow in the traps to trap the cells of interest in the traps, providing first molecules of interest to the traps, and providing an electric field across the traps to perform electroporation of the first molecules of interest into the cells of interest in the traps.

Abstract: A system and method include delivering cells of interest to multiple traps via a channel connecting the traps, maintaining a vortex flow in the traps to trap the cells of interest in the traps, providing first molecules of interest to the traps, and providing an electric field across the traps to perform electroporation of the first molecules of interest into the cells of interest in the traps.

Abstract: A system and method include delivering cells of interest to multiple traps via a channel connecting the traps, maintaining a vortex flow in the traps to trap the cells of interest in the traps, providing first molecules of interest to the traps, and providing an electric field across the traps to perform electroporation of the first molecules of interest into the cells of interest in the traps.