Abstract: This application provides methods and compositions related to constructing nucleic acid hydrogels (e.g., RNA hydrogels) having repetitive monomer units, each monomer unit includes one or more G-quadruplex sequences. These G-quadruplex sequences cross-link the nucleic acid concatemer such that it self-assembles into a hydrogel under appropriate conditions. In some embodiments, each monomeric unit of the nucleic acid concatemer comprises a coding sequence for polypeptide of interest; and the nucleic acid hydrogel formed by the nucleic acid concatemer can be used for expressing the polypeptide in high quantities. In some embodiments, at least two RNA concatemers comprising G-quadruplex sequences are produced, one further comprising a spacer and the other further comprising a sequence encoding a polypeptide of interest. These two RNA concatemers are combined and self assembled to form a single, wideband RNA hydrogel.

Abstract: This application provides methods and compositions related to constructing nucleic acid hydrogels (e.g., RNA hydrogels) having repetitive monomer units, each monomer unit includes one or more G-quadruplex sequences. These G-quadruplex sequences cross-link the nucleic acid concatemer such that it self-assembles into a hydrogel under appropriate conditions. In some embodiments, each monomeric unit of the nucleic acid concatemer comprises a coding sequence for polypeptide of interest; and the nucleic acid hydrogel formed by the nucleic acid concatemer can be used for expressing the polypeptide in high quantities. In some embodiments, at least two RNA concatemers comprising G-quadruplex sequences are produced, one further comprising a spacer and the other further comprising a sequence encoding a polypeptide of interest. These two RNA concatemers are combined and self assembled to form a single, wideband RNA hydrogel.

Abstract: The present invention relates to a nucleic acid probe system for increasing the detection efficiency of nucleic acid biomarkers, and improves the detection efficiency and detection sensitivity of biomarkers through a method for integrating a single-stranded probe sequence into a nucleic acid nanostructure. A nucleic acid nanostructure of the present invention, developed for this, converts, to a fast reaction inside the structure, some collision/reaction steps between molecules according to simple diffusion through localized aggregation of probe sequences, activates a fast signal amplification mechanism in the structure through structural flexibility so as to improve detection ability, thereby having effects of improving detection ability and effectively lowering a detection limit.

Abstract: The present disclosure relates to a polymer-iron oxide composite nanoparticle, a polymer-iron oxide composite nanoparticle including a silica coating layer coated on the surface of the polymer-iron oxide composite nanoparticle, a DNA-containing polymer-iron oxide composite nanostructure including DNA attached on the silica coating layer, a method of preparing the same and a method of controlling expression of a gene.

Abstract: This application provides methods and compositions related to constructing nucleic acid hydrogels (e.g., RNA hydrogels) having repetitive monomer units, each monomer unit includes one or more G-quadruplex sequences. These G-quadruplex sequences cross-link the nucleic acid concatemer such that it self-assembles into a hydrogel under appropriate conditions. In some embodiments, each monomeric unit of the nucleic acid concatemer comprises a coding sequence for polypeptide of interest; and the nucleic acid hydrogel formed by the nucleic acid concatemer can be used for expressing the polypeptide in high quantities. In some embodiments, at least two RNA concatemers comprising G-quadruplex sequences are produced, one further comprising a spacer and the other further comprising a sequence encoding a polypeptide of interest. These two RNA concatemers are combined and self assembled to form a single, wideband RNA hydrogel.

Abstract: The present invention relates to a multiple-nucleic-acid probe comprising a single nucleotide sequence that binds to biomarkers for detection of multiple nucleic acids. An artificial nucleotide sequence designed by the method for designing an artificial nucleotide sequence according to the present invention exhibits better hybridization reactivity with all analogs, and a multiple-nucleic-acid probe comprising the artificial nucleotide sequence is designed such that a single diagnostic probe is capable of simultaneously binding to multiple types of nucleic acids having significance. Thus, use of the single diagnostic probe may achieve ultra-multiplex diagnosis of nucleic acid biomarkers by diagnosing the overall expression pattern in a sample, whereby diagnostic ability may be improved and the cost of examination may be drastically reduced.

Abstract: The present invention relates to a graphene oxide complex having attached thereto a structure composed of single-stranded probes, each comprising a nucleic acid complementary to a target nucleic acid and a fluorescent substance. The graphene oxide complex can detect and quantify multiple biomarkers (target nucleic acids) in real time with high sensitivity at low cost without PCR, and thus can easily diagnose most diseases in which multiple biomarkers are involved, and evaluate the effect of drug use.

Abstract: A method of preparing a base sequence for synthesis of a nucleic acid nanostructure, a method of synthesizing a nucleic acid nanostructure, a candidate base sequence pair, and a nucleic acid nanostructure are provided. A method of preparing a base sequence includes selecting candidate groups of base sequences of 5?-cohesive end having at least 50% GC content, calculating Gibb's free energy values of the selected candidate groups to re-select the candidate groups, and selecting the candidate groups, that have a sequence of 3 different successive bases among the base sequences from the re-selected candidate groups.

Abstract: The present disclosure relates to a polymer-iron oxide composite nanoparticle, a polymer-iron oxide composite nanoparticle including a silica coating layer coated on the surface of the polymer-iron oxide composite nanoparticle, a DNA-containing polymer-iron oxide composite nanostructure including DNA attached on the silica coating layer, a method of preparing the same and a method of controlling expression of a gene.

Abstract: The present disclosure relates to a method of treating cancer using a composition including a target-specific self-illuminative nanocomplex which includes a self-illuminative nanocomplex including a quantum dot and a modified self-illuminative protein (m-Rluc8), a targeted cancer-specific molecule and a hydrophilic polymer, and a method of preparing the composition. Since the high efficiency self-illuminative nanocomplex may have high target specificity by chemically binding to a target-specific molecule, it can be effectively used in cancer treatment using a nanometer-scale laser light source, and therefore it is expected to propose a new paradigm of nanoptic therapeutics.

Abstract: The present invention relates to a functional complex particle prepared by filling a nucleic acid hydrogel inside a liposome and a method of producing the same. The present invention may have an effect of increasing an expression of protein factors included in the particle by incorporating an X-shaped nucleic acid monomer in the nucleic acid hydrogel. Accordingly, when the core-shell particle is prepared using the method according to an embodiment of the present invention, an effect of facilitating an introduction of a genome into the nucleic acid hydrogel may be obtained, and thereby the core-shell particle may be used as a protein production platform copying a cell nucleus.

Abstract: The present disclosure relates to a method of treating cancer using a composition including a target-specific self-illuminative nanocomplex which includes an self-illuminative nanocomplex including a quantum dot and a modified self-illuminative protein (m-Rluc8), a targeted cancer-specific molecule and a hydrophilic polymer, and a method of preparing the composition. Since the high efficiency self-illuminative nanocomplex may have high target specificity by chemically binding to a target-specific molecule, it can be effectively used in cancer treatment using a nanometer-scale laser light source, and therefore it is expected to propose a new paradigm of nanoptic therapeutics.

Abstract: The present invention relates to a multi-functional nucleic acid-based anti-cancer drug in which the anti-cancer drug is physically bound to a linear nucleic acid having a thiol group at 5? terminal thereof and then gold nanoparticles and aptamers are chemically bound. The present invention also relates to a method for preparing the anti-cancer drug and to an anti-cancer composition comprising the anti-cancer drug. The multi-functional nucleic acid-based anti-cancer drug according to the present invention uses A10 aptamer to achieve high targeting properties, and uses high-concentration anti-cancer drugs and gold nanoparticles to enable dual therapy of thermal therapy/chemical therapy, and may have less side effects and be more effective in anti-cancer therapy compared to existing anti-cancer drugs.

Abstract: The present invention relates to a functional complex particle prepared by filling a nucleic acid hydrogel inside a liposome and a method of producing the same. The present invention may have an effect of increasing an expression of protein factors included in the particle by incorporating an X-shaped nucleic acid monomer in the nucleic acid hydrogel. Accordingly, when the core-shell particle is prepared using the method according to an embodiment of the present invention, an effect of facilitating an introduction of a genome into the nucleic acid hydrogel may be obtained, and thereby the core-shell particle may be used as a protein production platform copying a cell nucleus.

Abstract: A method of preparing a base sequence for synthesis of a nucleic acid nanostructure, a method of synthesizing a nucleic acid nanostructure, a candidate base sequence pair, and a nucleic acid nanostructure are provided. A method of preparing a base sequence includes selecting candidate groups of base sequences of 5?-cohesive end having at least 50% GC content, calculating Gibb's free energy values of the selected candidate groups to re-select the candidate groups, and selecting the candidate groups, that have a sequence of 3 different successive bases among the base sequences from the re-selected candidate groups.

Abstract: The present disclosure relates to a fusion nano liposome-fluorescence labeled nucleic acid in which a bead having a surface binding with a branch-shaped nucleic acid structure labeled with a fluorophore or a branch-shaped nucleic acid structure having a hairpin loop end is included in an inside of a liposome, and a diagnosis application thereof. The fusion nano liposome-fluorescence labeled nucleic acid, or fusion nano liposome-fluorescence labeled hairpin loop structured nucleic acid may sense an external or internal signal, and high-sensitive diagnosis is possible even when mRNA and miRNA which is present at a low concentration in cells being targeted. Further, various target materials expressed inside and outside of a cell membrane may be targeted, and thus even a type of cancer which is hard to diagnose such as triple negative breast cancer also be flexibly diagnosed. Further, using various fluorophores, multiple cancer may be diagnosed at the same time.

Abstract: An artificial lipid-polymer-DNA complex, an in-vivo imaging agent, a pharmaceutical composition and a method of preparing the same are provided. The artificial lipid bilayer includes a polymer matrix including polylactic-co-glycolic acid disposed within a lipid membrane.

Abstract: The present disclosure relates to functional composite particles produced by filling water-soluble or lipid-soluble polymers into changeable liposomes and a method of producing the same. The present disclosure also relates to an evaluation of specialized biochemical characteristics of composites after the composites are produced using the water-soluble or lipid-soluble polymers by selection from groups capable of combining with lipid layers. A protocol according to an embodiment of the present disclosure overcomes the limitations of a conventional water/oil-based single emulsion protocol to prepare single polymer particles or single lipid layered particles and combine liposomes with a variety of polymer groups.

Abstract: Provided are a nucleic acid gel matrix for the cell-free protein synthesis of a cell nucleus replicate that contains an X-type nucleic acid nanostructure, an expression plasmid containing a DNA fragment, transcription and translation constituents, and a lipid membrane component, and a method for producing same. The nucleic acid gel matrix for the cell-free synthesis of a cell nucleus replicate is a polymorphic gel matrix that contains the X-type nucleic acid nanostructure, the expression plasmid containing the DNA fragment, the transcription and translation constituents, and the lipid membrane component.

Abstract: An artificial lipid-polymer-DNA complex, an in-vivo imaging agent, a pharmaceutical composition and a method of preparing the same are provided. The artificial lipid bilayer includes a polymer matrix including polylactic-co-glycolic acid disposed within a lipid membrane.