Abstract: The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus Ogataea, a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant. Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.

Abstract: The present invention relates to a peptide excellently capable of binding to a protein capable of binding to insulin (IBP), an adsorbent comprising the aforementioned peptide immobilized on a carrier, an adsorber comprising the aforementioned adsorbent, and a method of adsorbing IBPs by using the aforementioned adsorbent or adsorber. The peptide, adsorbent, adsorber and method provided by the present invention can selectively adsorb and remove IBPs efficiently, and therefore be used, for example, in the treatment of diseases, typically diabetes, in which such IBP acts as an aggravating factor.

Abstract: The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus Ogataea, a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant. Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.

Abstract: The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus Ogataea, a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant. Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.

Abstract: Disclosed are: a method for producing an anti-sense nucleic acid which has a wide variation in nucleotide sequences and excellent anti-sense properties at a low cost in a simple manner and in a large scale; and a cosmetic composition or pharmaceutical composition comprising the anti-sense nucleic acid. A method is discovered which can produce a low molecular weight nucleic acid having anti-sense properties from a nucleic acid starting material derived from an organism at a high yield by using a probe nucleic acid having at least a part of the nucleotide sequence of a target gene. An anti-sense nucleic acid produced by using a nucleic acid derived from an organism as a starting material; and a method for producing the anti-sense nucleic acid.

Abstract: The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus Ogataea, a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant. Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.

Abstract: The present invention relates to a peptide excellently capable of binding to a protein capable of binding to insulin (IBP), an adsorbent comprising the aforementioned peptide immobilized on a carrier, an adsorber comprising the aforementioned adsorbent, and a method of adsorbing IBPs by using the aforementioned adsorbent or adsorber. The peptide, adsorbent, adsorber and method provided by the present invention can selectively adsorb and remove IBPs efficiently, and therefore be used, for example, in the treatment of diseases, typically diabetes, in which such IBP acts as an aggravating factor.

Abstract: A disease treatment is provided by controlling the expression of a protein induced by a heat shock factor. The novel compound benzo-1,3-dioxole provides an inhibitor of HSF activity or an inhibitor of inducing the production of a protein regulated by HSF, which inhibits the activity of a heat shock factor, a transcriptional regulatory factor, thereby in turn inhibiting transcription of a structural gene having a heat shock element sequenc in the gene region for transcriptional regulation into RNA, and thus inhibiting translation of the gene into a protein, and resulting in inhibition of inducing production of RNA or protein encoded by the gene. It also provides a drug for treating or preventing cancer through thermotherapy and a drug for treating or preventing stress diseases such as depression.

Abstract: An enzyme modifying method for converting the coenzyme-dependency of an oxidoreductase is developed. Using this method, a novel carbonyl reductase mutant capable of utilizing NADH as a coenzyme is provided. It is also intended to provide a process for enzymatically producing an optically active (S)-4-halo-3-hydroxybutyric ester by utilizing the carbonyl reductase mutant.

Abstract: The present invention provides a method for calculating an optimized solution of the amino acid sequences of multiple-mutated proteins. The method comprises searching the three-dimensional structural coordinates of amino acid side chains of the amino acid sequences of members of a multiple-mutated protein population based on the three-dimensional structure data of a template protein population using a dead end elimination algorithm, and executing structural energy minimization calculations for the members, thereby calculating the three-dimensional structural coordinates of an optimum multiple-mutated protein, calculating a characteristic value from the three-dimensional structural coordinates of the optimum multiple-mutated protein, and applying a genetic algorithm to the multiple-mutated protein population to calculate the members which optimize the characteristic value.

Abstract: A disease treatment is provided by controlling the expression of a protein induced by a heat shock factor. The novel compound benzo-1,3-dioxole provides an inhibitor of HSF activity or an inhibitor of inducing the production of a protein regulated by HSF, which inhibits the activity of a heat shock factor, a transcriptional regulatory factor, thereby in turn inhibiting transcription of a structural gene having a heat shock element sequence in the gene region for transcriptional regulation into RNA, and thus inhibiting translation of the gene into a protein, and resulting in inhibition of inducing production of RNA or protein encoded by the gene. It also provides a drug for treating or preventing cancer through thermotherapy and a drug for treating or preventing stress diseases such as depression.

Abstract: A disease treatment is provided by controlling the expression of a protein induced by a heat shock factor.

Abstract: A disease treatment is provided by controlling the expression of a protein induced by a heat shock factor.

Abstract: A disease treatment is provided by controlling the expression of a protein induced by a heat shock factor. The novel compound benzo-1,3-dioxole provides an inhibitor of HSF activity or an inhibitor of inducing the production of a protein regulated by HSF, which inhibits the activity of a heat shock factor, a transcriptional regulatory factor, thereby in turn inhibiting transcription of a structural gene having a heat shock element sequence in the gene region for transcriptional regulation into RNA, and thus inhibiting translation of the gene into a protein, and resulting in inhibition of inducing production of RNA or protein encoded by the gene. It also provides a drug for treating or preventing cancer through thermotherapy and a drug for treating or preventing stress diseases such as depression.

Abstract: The present invention has for its object to provide a medicinal composition comprising coenzyme Q10 as an active ingredient, which composition features an enhanced absorption after oral administration. The present invention is directed to a medicinal composition comprising coenzyme Q10 as an active ingredient with the reduced form of coenzyme Q10 accounting for more than 20 weight % of said coenzyme Q10.