Patents by Inventor Sousuke Miyoshi

Sousuke Miyoshi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of transferring a selected molecule into a cell
    Patent number: 7402435
    Abstract: A method of highly efficiently transferring various selected molecules into various cells and a method of fusing cells. Cells and/or selected molecules such as polynucleotide are treated with cold gas plasma to thereby transfer the selected molecules located around cells into the cells, or cells are fused by treating the cells with cold gas plasma. Moreover, an apparatus for transferring selected molecules or fusing cells having a cold gas plasma generation unit for transferring selected molecules into cells is provided.
    Type: Grant
    Filed: February 8, 2002
    Date of Patent: July 22, 2008
    Assignees: BBK Bio Corporation, Pearl Kogyo Co., Ltd.
    Inventors: Sousuke Miyoshi, Akiko Ohkubo, Noriyuki Morikawa, Yasuhiro Ogawa, Shintaro Nishimura, Masao Fukagawa, Hiroyuki Arakawa, Jyunko Zenkou, Susumu Sato
  • Molecule transferring device, auxiliary for molecule transferring device, and molecule transferring method
    Patent number: 7341864
    Abstract: A molecule introducing apparatus is provided with a power supply circuit 20 for supplying power, a step-up transformer 30 which is supplied with power from the power supply circuit 20 and which outputs a high voltage, a switching transistor 22 that blocks off and allows the supply of power from the power supply circuit 20 to the step-up transformer 30 and which generates an instantaneous high voltage, and a pair of electrode probes 50 for applying the instantaneous high voltage generated at the step-up transformer 30 to a predetermined region in a body. The molecule introducing apparatus oscillates DNA arranged outside a cell by the instantaneous high voltage so as to introduce the DNA into the cell.
    Type: Grant
    Filed: January 29, 2001
    Date of Patent: March 11, 2008
    Assignees: The Hollenniun Laboratories, Meiryo Technica Corporation, Ltd.
    Inventors: Yoshitaka Sakamoto, Masafumi Koide, Juichiro Nakashima, Susumu Satoh, Sousuke Miyoshi, Akiko Suzuki, Hiroyuki Arakawa, Hiroshi Marusawa
  • Method of transffering selected molecules
    Publication number: 20040110297
    Abstract: A method of highly efficiently transferring various selected molecules into various cells and a method of fusing cells. Cells and/or selected molecules such as polynucleotide are treated with cold gas plasma to thereby transfer the selected molecules located around cells into the cells, or cells are fused by treating the cells with cold gas plasma. Moreover, an apparatus for transferring selected molecules or fusing cells having a cold gas plasma generation unit for transferring selected molecules into cells is provided.
    Type: Application
    Filed: February 2, 2004
    Publication date: June 10, 2004
    Inventors: Sousuke Miyoshi, Akiko Ohkubo, Noriyuki Morikawa, Yasuhiro Ogawa, Shintaro Nishimura, Masao Fukagawa, Hiroyuki Arakawa, Jyunko Zenkou, Susumu Sato
  • Transmembrane trapping methods
    Patent number: 6582905
    Abstract: The present invention provides a method of cloning a protein coding for a membrane protein without the need of using anyantibody or ligand. The method of cloning a membrane protein comprises selecting a protein having a signal sequence, using a DNA coding for the signal sequence and N-terminal sequence of the protein as the reporter gene, and screening for a gene for a protein having a transmembrane domain(s). The present invention is concerned also with the CDNA obtained by using such method, a vector containing the DNA, a transformant as transformed with the vector, and the transcription product of the DNA as produced by the transformant.
    Type: Grant
    Filed: March 15, 1999
    Date of Patent: June 24, 2003
    Inventors: Hiromitsu Nakauchi, Yukio Nakamura, Sousuke Miyoshi, Dong ku Kim
  • Molecule transferring device, auxiliary for molecule transferring device, and molecule transferring method
    Publication number: 20030092182
    Abstract: A molecule introducing apparatus is provided with a power supply circuit 20 for supplying power, a step-up transformer 30 which is supplied with power from the power supply circuit 20 and which outputs a high voltage, a switching transistor 22 that blocks off and allows the supply of power from the power supply circuit 20 to the step-up transformer 30 and which generates an instantaneous high voltage, and a pair of electrode probes 50 for applying the instantaneous high voltage generated at the step-up transformer 30 to a predetermined region in a body. The molecule introducing apparatus oscillates DNA arranged outside a cell by the instantaneous high voltage so as to introduce the DNA into the cell.
    Type: Application
    Filed: November 13, 2002
    Publication date: May 15, 2003
    Inventors: Yoshitaka Sakamoto, Masafumi Koide, Juichiro Nakashima, Susumu Satoh, Sousuke Miyoshi, Akiko Suzuki, Hiroyuki Arakawa, Hiroshi Marusawa