Patents by Inventor Srabani Bhaumik

Srabani Bhaumik has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210270704
    Abstract: This disclosure relates generally to the use of automated platforms in the preparation of biomarker-stained cellular samples for microscopic analysis and use of such stained cells in the diagnosis of certain conditions. Disclosed herein is a method of affinity staining a Romanowsky-type stained sample on automated advanced staining systems, wherein the automated advanced stainer destains the sample prior to contact with a biomarker-specific reagent. Also disclosed herein are methods of processing body fluid samples for morphological and biomarker analysis by depositing cells of the sample in a thin layer onto one or more solid supports, staining at least one such solid support with a Romanowsky-type stain and staining at least one such solid support for one or more biomarkers useful for categorizing one or more cells of the sample.
    Type: Application
    Filed: May 19, 2021
    Publication date: September 2, 2021
    Inventors: Srabani Bhaumik, Simon J. Davidson, Jerome W. Kosmeder, II, Katherine K. Mui, Esteban Roberts, Noemi Sebastiao, David J. Zahniser
  • Patent number: 10920287
    Abstract: An expression vector, comprising a first reporter nucleic acid sequence operably linked to a first expression control sequence comprising a promoter; and a second reporter nucleic acid sequence operably linked to a second expression control sequence that comprises a response element that is activated or inactivated as one or more of the cells differentiate or dedifferentiate. Methods and kits for imaging and monitoring stem cells comprising the expression vector are also provided.
    Type: Grant
    Filed: June 23, 2017
    Date of Patent: February 16, 2021
    Assignees: Global Life Sciences Solutions Operations UK Ltd, The Board of Trustees of the Leland Stanford Junior University
    Inventors: Srabani Bhaumik, Sanjiv Sam Gambhir, Ramasamy Paulmurugan, Shahriar Yaghoubi, Byeong-Cheol Ahn, Natesh Parashurama
  • Publication number: 20170298455
    Abstract: An expression vector, comprising a first reporter nucleic acid sequence operably linked to a first expression control sequence comprising a promoter; and a second reporter nucleic acid sequence operably linked to a second expression control sequence that comprises a response element that is activated or inactivated as one or more of the cells differentiate or dedifferentiate. Methods and kits for imaging and monitoring stem cells comprising the expression vector are also provided.
    Type: Application
    Filed: June 23, 2017
    Publication date: October 19, 2017
    Inventors: Srabani BHAUMIK, Sanjiv Sam GAMBHIR, Ramasamy PAULMURUGAN, Shahriar YAGHOUBI, Byeong-Cheol AHN, Natesh PARASHURAMA
  • Patent number: 9778263
    Abstract: The present disclosure relates to characterization of biological samples. By way of example, a biological sample may be contacted with a plurality of probes specific for targets in the sample, such as probes for immune markers and segmenting probes. Acquired image data of the sample may be used to segment the images into epithelial and stromal regions to characterize individual cells in the sample based on the binding of the probes. Further, the biological sample may be characterized by a distribution, location, and type of a plurality of the characterized cells.
    Type: Grant
    Filed: November 13, 2013
    Date of Patent: October 3, 2017
    Assignee: General Electric Company
    Inventors: Srabani Bhaumik, Michael John Gerdes, Zhengyu Pang, Fiona Ginty, Christopher James Sevinsky, Qing Li, Alberto Santamaria-Pang, Yunxia Sui, Keyur Hemantkumar Desai
  • Patent number: 9719146
    Abstract: An expression vector, comprising a first reporter nucleic acid sequence operably linked to a first expression control sequence comprising a promoter; and a second reporter nucleic acid sequence operably linked to a second expression control sequence that comprises a response element that is activated or inactivated as one or more of the cells differentiate or dedifferentiate. Methods and kits for imaging and monitoring stem cells comprising the expression vector are also provided.
    Type: Grant
    Filed: September 9, 2009
    Date of Patent: August 1, 2017
    Assignee: General Electric Company
    Inventors: Srabani Bhaumik, Sanjiv Sam Gambhir, Ramasamy Paulmurugan, Shahriar Yaghoubi, Byeong-Cheol Ahn, Natesh Parashurama
  • Publication number: 20150133321
    Abstract: The present disclosure relates to characterization of biological samples. By way of example, a biological sample may be contacted with a plurality of probes specific for targets in the sample, such as probes for immune markers and segmenting probes. Acquired image data of the sample may be used to segment the images into epithelial and stromal regions to characterize individual cells in the sample based on the binding of the probes. Further, the biological sample may be characterized by a distribution, location, and type of a plurality of the characterized cells.
    Type: Application
    Filed: November 13, 2013
    Publication date: May 14, 2015
    Applicant: General Electric Company
    Inventors: Srabani Bhaumik, Michael John Gerdes, Zhengyu Pang, Fiona Ginty, Christopher James Sevinsky, JR., Qing Li, Alberto Santamaria-Pang, Yunxia Sui, Keyur Hemantkumar Desai
  • Patent number: 8021647
    Abstract: Provided herein are methods for imaging an optically labeled target cell implanted in vivo using a reporter construct that encodes an enzyme that is not endogenous to the target cell. The disclosed methods include the steps of: (a) introducing target cell transformed with non-endogenous enzyme into a mammalian subject; (b) contacting the transformed target cell with a cell-permeable soluble fluorescent dye; and (c) observing a fluorescent signal generated by the target cell present in the mammalian subject. In some embodiments, the non-endogenous enzyme comprises a nitroreductase. In some embodiments, the methods may also include the step of tracking the fluorescent target cells in the mammalian subject over time.
    Type: Grant
    Filed: June 29, 2007
    Date of Patent: September 20, 2011
    Assignee: General Electric Company
    Inventors: Srabani Bhaumik, Eric Dustin Agdeppa, June Klimash, Jeannette Christine DePuy
  • Patent number: 7906106
    Abstract: Provided herein are methods of monitoring inflammatory cell migration in a mammal. The methods include the steps of: providing a donor mammalian subject; introducing an inflammatory agent into the donor mammal to create a granuloma; isolating granuloma-derived nucleated cells from the granuloma of the donor mammalian subject; labeling the granuloma-derived nucleated cells with an optical agent; providing a recipient mammalian subject with inflamed tissue; introducing the granuloma-derived nucleated cells into a recipient mammalian subject at a site remote from site where the inflammatory agent was introduced; and imaging the recipient mammalian subject using an optical system. The methods may also include analysing the distribution of the labeled granuloma-derived cells in the recipient mammal.
    Type: Grant
    Filed: June 27, 2007
    Date of Patent: March 15, 2011
    Assignee: General Electric Company
    Inventors: Eric Dustin Agdeppa, Srabani Bhaumik, June Klimash, Jeannette Christine DePuy, Anton Beletskii
  • Publication number: 20110059439
    Abstract: An expression vector, comprising a first reporter nucleic acid sequence operably linked to a first expression control sequence comprising a promoter; and a second reporter nucleic acid sequence operably linked to a second expression control sequence that comprises a response element that is activated or inactivated as one or more of the cells differentiate or dedifferentiate. Methods and kits for imaging and monitoring stem cells comprising the expression vector are also provided.
    Type: Application
    Filed: September 9, 2009
    Publication date: March 10, 2011
    Applicant: GENERAL ELECTRIC COMPANY
    Inventors: Srabani Bhaumik, Sanjiv Sam Gambhir, Ramasamy Paulmurugan, Shahriar Yaghoubi, Byeong-Cheol Ahn, Natesh Parashurama
  • Publication number: 20090004116
    Abstract: Provided herein are methods for imaging an optically labeled target cell implanted in vivo using a reporter construct that encodes an enzyme that is not endogenous to the target cell. The disclosed methods include the steps of: (a) introducing target cell transformed with non-endogenous enzyme into a mammalian subject; (b) contacting the transformed target cell with a cell-permeable soluble fluorescent dye; and (c) observing a fluorescent signal generated by the target cell present in the mammalian subject. In some embodiments, the non-endogenous enzyme comprises a nitroreductase. In some embodiments, the methods may also include the step of tracking the fluorescent target cells in the mammalian subject over time.
    Type: Application
    Filed: June 29, 2007
    Publication date: January 1, 2009
    Applicant: GENERAL ELECTRIC COMPANY
    Inventors: Srabani Bhaumik, Eric Dustin Agdeppa, June Klimash, Jeannette Christine DePuy
  • Publication number: 20090004115
    Abstract: Provided herein are methods of monitoring inflammatory cell migration in a mammal. The methods include the steps of: providing a donor mammalian subject; introducing an inflammatory agent into the donor mammal to create a granuloma; isolating granuloma-derived nucleated cells from the granuloma of the donor mammalian subject; labeling the granuloma-derived nucleated cells with an optical agent; providing a recipient mammalian subject with inflamed tissue; introducing the granuloma-derived nucleated cells into a recipient mammalian subject at a site remote from site where the inflammatory agent was introduced; and imaging the recipient mammalian subject using an optical system. The methods may also include analysing the distribution of the labeled granuloma-derived cells in the recipient mammal.
    Type: Application
    Filed: June 27, 2007
    Publication date: January 1, 2009
    Applicant: GENERAL ELECTRIC COMPANY
    Inventors: Eric Dustin Agdeppa, Srabani Bhaumik, June Klimash, Jeannette Christine DePuy, Anton Beletskii