Abstract: The present invention relates to a method of inhibiting, delaying, or reversing cellular senescence that includes having an isolated cell flow through a microchannel, and crashing the cell in flow into an impact surface installed on a flow path of the cell to apply a physical impact to the cell, resulting in inhibiting, delaying, or reversing senescence of the cell, while maintaining high biological activity, and in particular, it relates to a method that can further increase the value of a stem cell as a therapeutic cell for various degenerative diseases by maintaining undifferentiated state of the stem cell even during a long-term culture period to maintain multipotency, and to the cells obtained by the method.

Abstract: The present disclosure relates to a method for mass production of extracellular vesicles by using a noxa protein-derived peptide and mesenchymal stem cells and, more specifically, to a method for mass production of extracellular vesicles having wound healing and immunomodulatory effects, wherein mesenchymal stem cells are cultured in a medium composition containing a noxa protein-derived peptide, glucose, sucrose, and 3-(N-morpholino)propanesulfonic acid [MOPS], whereby the extracellular vesicles can be obtained at high yield with high purity.

Abstract: The present disclosure relates to a method for producing mesenchymal stem cells from pluripotent stem cells and mesenchymal stem cells prepared by the method. The present disclosure enables mesenchymal stem cells having superior proliferation rate while having superior intrinsic biological activity to be obtained with high yield by sequentially performing three-dimensional suspension culture using pluripotent stem cells, particularly induced pluripotent stem cells (iPSCs), as starting cells and adherent culture of cell aggregates formed therethrough. The mesenchymal stem cells produced by the method of the present disclosure can be usefully used in compositions for treating bone diseases, cartilage diseases or inflammatory and autoimmune diseases owing to high differentiation efficiency into bone and cartilage and superior anti-inflammatory activity.

Abstract: The present disclosure relates to a method for producing extracellular vesicles from three-dimensionally cultured stem cells. The method of the present disclosure can produce stem cell-derived extracellular vesicles with a high yield through orbital shaking culture of stem cell aggregates in the presence of TGF-? and thus can be usefully used in an industrial-scale mass production process of exosomes that can be utilized as a pharmaceutical ingredient substituting for a cell therapeutic agent. Furthermore, the exosomes obtained by the method of the present disclosure have significantly improved immunoregulatory functions as compared to the exosomes produced by the existing method and, therefore, can be applied as a superior therapeutic composition for various inflammations or autoimmune diseases.

Abstract: The present disclosure relates to a composition containing extracellular vesicles isolated from skeletal muscle-derived stem cells as active ingredients, which exhibits the effect of skin aging delay, wound healing, scar reduction and skin whitening, thereby improving skin condition. The present disclosure can not only utilize tissues wasted after surgical operation, thus allowing easy supply of raw materials, but also takes advantage of natural substances, thereby being free from the risk of side effects even upon long-term administration. The composition of the present disclosure promotes the secretion of collagen, reduces reactive oxygen species and promotes re-epithelialization at wound site while remarkably inhibiting melanocyte proliferation, tyrosinase activity and melanogenesis. Accordingly, it can facilitate effective recovery from skin tissue damage caused by oxidative stress, physical wound, intrinsic aging at cell or tissue levels, or hyperpigmentation.

Abstract: The present invention relates to a method of inhibiting, delaying, or reversing cellular senescence that includes having an isolated cell flow through a microchannel, and crashing the cell in flow into an impact surface installed on a flow path of the cell to apply a physical impact to the cell, resulting in inhibiting, delaying, or reversing senescence of the cell, while maintaining high biological activity, and in particular, it relates to a method that can further increase the value of a stem cell as a therapeutic cell for various degenerative diseases by maintaining undifferentiated state of the stem cell even during a long-term culture period to maintain multipotency, and to the cells obtained by the method.

Abstract: This disclosure provides for a three-dimensional (3D) microenvironment presenting defined physical or mechanical cues that regulate cellular behavior and use of the matrix. The disclosure also provides for devices and methods for screening for optimal combinations of physical and mechanical cues in order to create a microenvironment that can regulate specific cellular behavior such as cell growth, proliferation, migration or differentiation.

Abstract: This disclosure provides for a three-dimensional (3D) microenvironment presenting defined physical or mechanical cues that regulate cellular behavior and use of the matrix. The disclosure also provides for devices and methods for screening for optimal combinations of physical and mechanical cues in order to create a microenvironment that can regulate specific cellular behavior such as cell growth, proliferation, migration or differentiation.

Abstract: Disclosed is a method for manufacturing stem cells including preparing Oct-4 gene, Sox2 gene, C-myc gene, and Klf-4 gene from mouse embryonic stem cells, and allowing each of the genes to be infected in host cells using a lentiviral vector system to generate viruses in which each of the genes are induced; concentrating or mixing each of the viruses to prepare a virus concentrated mixture, and mixing the virus concentrated mixture and a first culture solution to prepare a virus solution; floating mouse somatic cells having been cultivated in advance in a first culture dish, and mixing and reacting the floated somatic cells and the virus solution to prepare a somatic cell-virus mixture; adding and retaining the somatic cell-virus mixture as is in a second culture dish including a second culture solution to induce the genes in the somatic cells; and cultivating the somatic cells.